FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_59.3410000000a30e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_59.3410000000a30e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC17310.574572127139364No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT181.1002444987775062No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC181.1002444987775062RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC150.9168704156479218TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG80.4889975550122249No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC50.3056234718826406No Hit
CTTATACACCATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT30.18337408312958436No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG30.18337408312958436No Hit
CTTATACACATCTCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT30.18337408312958436RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.12224938875305623RNA PCR Primer, Index 27 (95% over 21bp)
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG20.12224938875305623No Hit
CCCCATGGCCTTTCGCCTCTGAAAGCGTCCTTCCTTCTCAATGCCCGGGCA20.12224938875305623No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT20.12224938875305623No Hit
CTTATACACATCTCCTAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC20.12224938875305623No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA20.12224938875305623No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC20.12224938875305623No Hit
GTAAGTATCTGTTAAGCTCATTTATTCTAGGCTGTAGTTTAAGTCTATTGT20.12224938875305623No Hit
GTGTGAATGTTCCTTTTTTTTTAGTTTCCTTTTGGATTGGATGAACTATGA20.12224938875305623No Hit
TATATGTTGGGACCCGAAAGATGGTGAACTATGCCTGAATCTGTCTCTTAT20.12224938875305623No Hit
ACATGGAATATGGCAAAAAATCTGAAAATCATAGAAAATGAGAAACATCCA20.12224938875305623No Hit
CTTATACACATCTACGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC20.12224938875305623No Hit
CTATTCTATAGAACTCTAACTGCTTGTAAGGCTTGTAATTCAAGATCTATT20.12224938875305623No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)