FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_58.3410000000a2f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_58.3410000000a2f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC13012.18369259606373No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT191.7806935332708531No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC70.6560449859418931RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC50.4686035613870665TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG40.37488284910965325No Hit
CTTATACACATTCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT20.18744142455482662No Hit
CCTGCTAAGTGAGTGAACAAAATTTGCTAATATCAATTAATGACCTGCATG20.18744142455482662No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA20.18744142455482662No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC20.18744142455482662No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT251.822089E-545.00000432
CCTATCT251.822089E-545.00000434
TCCTATC251.822089E-545.00000433
TGCCGTC251.822089E-545.00000445
TATCTCG251.822089E-545.00000436
GATCCTA251.822089E-545.00000431
CTAGATC251.822089E-545.00000428
ACTAGAT251.822089E-545.00000427
AGACTAG251.822089E-545.00000425
AGATCCT251.822089E-545.00000430
TAGATCC251.822089E-545.00000429
CTATCTC251.822089E-545.00000435
GACTAGA251.822089E-545.00000426
AGCCCAC305.36695E-537.49999617
GTATGCC305.36695E-537.49999642
ATCTCGT305.36695E-537.49999637
ATCTCCG305.36695E-537.49999610
CACATCT305.36695E-537.4999967
GCCCACG305.36695E-537.49999618
CCACGAG305.36695E-537.49999620