FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_57.3410000000a2e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_57.3410000000a2e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC655.4575986565911No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT110.9235936188077246No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC70.5877413937867338RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.5037783375314862TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT40.33585222502099077No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG30.2518891687657431No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC30.2518891687657431No Hit
AGGCAAACCGGGAGAAGTGAAACATCTCAGTAACCCGAGGAAAAGACATCA20.16792611251049538No Hit
CTCTATACGGAGTTACAAAAGAACATGTTAGACGAATCATCTGGAAAGATG20.16792611251049538No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC20.16792611251049538No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG20.16792611251049538No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTCTGTCTCTT20.16792611251049538No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC20.16792611251049538No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)