FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_56.3410000000a2d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_56.3410000000a2d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC966.981818181818182No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC161.1636363636363636RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT141.0181818181818183No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC141.0181818181818183No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.4363636363636364TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG60.4363636363636364No Hit
CTTATACACACTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT30.2181818181818182RNA PCR Primer, Index 40 (95% over 21bp)
GAACTGTATATATCGAAACAGTGATATGACAATAGAGAAGACAACTATAGT20.14545454545454545No Hit
TAATTGATGATGACGAATAATCTCTCCCACGGCTGCTTTTGTATTTAGAGA20.14545454545454545No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC20.14545454545454545No Hit
CTTATACACACTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.14545454545454545RNA PCR Primer, Index 27 (95% over 21bp)
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG20.14545454545454545No Hit
CTTAATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT20.14545454545454545No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)