FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_55.3410000000a2c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_55.3410000000a2c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC12210.562770562770563No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT211.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC131.1255411255411256RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC50.4329004329004329TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG40.3463203463203463No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG40.3463203463203463No Hit
CTTATACACATTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT20.17316017316017315RNA PCR Primer, Index 40 (95% over 21bp)
ATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACT20.17316017316017315No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATATCGTATGCCGTC20.17316017316017315No Hit
GTCTTAGACAGGGAAGAAAACAAAATACGGTATTTAGACTGTCTCTTATAC20.17316017316017315No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC20.17316017316017315No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC150.00979924544.99999617
GTATGCC150.00979924544.99999642
ATCTCGT150.00979924544.99999637
ATCTCCG150.00979924544.99999610
CACATCT150.00979924544.9999967
GCCCACG150.00979924544.99999618
TATACAC150.00979924544.9999963
TGCCGTC150.00979924544.99999645
CCACGAG150.00979924544.99999620
TATGCCG150.00979924544.99999643
CATCTCC150.00979924544.9999969
GAGCCCA150.00979924544.99999616
CACGAGA150.00979924544.99999621
CCCACGA150.00979924544.99999619
ACATCTC150.00979924544.9999968
TACACAT150.00979924544.9999965
ACACATC150.00979924544.9999966
TCTCGTA150.00979924544.99999638
ACGAGAC150.00979924544.99999622
ATGCCGT150.00979924544.99999644
Produced by FastQC (version 0.11.2)