Basic Statistics
Measure | Value |
---|---|
Filename | H23V3BCXX l01n01 kb2_2_53.3410000000a2a0.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1323 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC | 151 | 11.413454270597127 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT | 18 | 1.3605442176870748 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC | 6 | 0.45351473922902497 | TruSeq Adapter, Index 12 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG | 6 | 0.45351473922902497 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC | 5 | 0.3779289493575208 | RNA PCR Primer, Index 27 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC | 3 | 0.22675736961451248 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 2 | 0.15117157974300832 | No Hit |
CTTATACACATCTCCGAGCCCACGAGCCTTGCAGAATCTCGTATGCCGTCT | 2 | 0.15117157974300832 | RNA PCR Primer, Index 40 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT | 2 | 0.15117157974300832 | No Hit |
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCC | 2 | 0.15117157974300832 | No Hit |
GGTATCAACGCAGAGTACGGGGTATCAACTGTCTCTTATACACATCTCCGA | 2 | 0.15117157974300832 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTAGATCGTATCTCGTATGCCGT | 2 | 0.15117157974300832 | No Hit |
TATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTT | 2 | 0.15117157974300832 | RNA PCR Primer, Index 27 (95% over 23bp) |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAACTGTCTC | 2 | 0.15117157974300832 | No Hit |
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2 | 0.15117157974300832 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGCCCAC | 20 | 4.7411676E-4 | 45.0 | 17 |
GTATGCC | 20 | 4.7411676E-4 | 45.0 | 42 |
ATCTCGT | 20 | 4.7411676E-4 | 45.0 | 37 |
GCCCACG | 20 | 4.7411676E-4 | 45.0 | 18 |
TGCCGTC | 20 | 4.7411676E-4 | 45.0 | 45 |
CCACGAG | 20 | 4.7411676E-4 | 45.0 | 20 |
TATGCCG | 20 | 4.7411676E-4 | 45.0 | 43 |
GAGCCCA | 20 | 4.7411676E-4 | 45.0 | 16 |
CACGAGA | 20 | 4.7411676E-4 | 45.0 | 21 |
CCCACGA | 20 | 4.7411676E-4 | 45.0 | 19 |
TCTCGTA | 20 | 4.7411676E-4 | 45.0 | 38 |
ACGAGAC | 20 | 4.7411676E-4 | 45.0 | 22 |
ATGCCGT | 20 | 4.7411676E-4 | 45.0 | 44 |
CGTATGC | 20 | 4.7411676E-4 | 45.0 | 41 |
CGAGCCC | 20 | 4.7411676E-4 | 45.0 | 15 |
CCGAGCC | 20 | 4.7411676E-4 | 45.0 | 14 |
TCGTATG | 20 | 4.7411676E-4 | 45.0 | 40 |
TCTCCGA | 20 | 4.7411676E-4 | 45.0 | 11 |
CGAGACT | 20 | 4.7411676E-4 | 45.0 | 23 |
TCCGAGC | 20 | 4.7411676E-4 | 45.0 | 13 |