FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_53.3410000000a2a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_53.3410000000a2a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC15111.413454270597127No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT181.3605442176870748No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.45351473922902497TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG60.45351473922902497No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC50.3779289493575208RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC30.22675736961451248No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA20.15117157974300832No Hit
CTTATACACATCTCCGAGCCCACGAGCCTTGCAGAATCTCGTATGCCGTCT20.15117157974300832RNA PCR Primer, Index 40 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT20.15117157974300832No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCC20.15117157974300832No Hit
GGTATCAACGCAGAGTACGGGGTATCAACTGTCTCTTATACACATCTCCGA20.15117157974300832No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCGTATCTCGTATGCCGT20.15117157974300832No Hit
TATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTT20.15117157974300832RNA PCR Primer, Index 27 (95% over 23bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAACTGTCTC20.15117157974300832No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15117157974300832No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC204.7411676E-445.017
GTATGCC204.7411676E-445.042
ATCTCGT204.7411676E-445.037
GCCCACG204.7411676E-445.018
TGCCGTC204.7411676E-445.045
CCACGAG204.7411676E-445.020
TATGCCG204.7411676E-445.043
GAGCCCA204.7411676E-445.016
CACGAGA204.7411676E-445.021
CCCACGA204.7411676E-445.019
TCTCGTA204.7411676E-445.038
ACGAGAC204.7411676E-445.022
ATGCCGT204.7411676E-445.044
CGTATGC204.7411676E-445.041
CGAGCCC204.7411676E-445.015
CCGAGCC204.7411676E-445.014
TCGTATG204.7411676E-445.040
TCTCCGA204.7411676E-445.011
CGAGACT204.7411676E-445.023
TCCGAGC204.7411676E-445.013