FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_52.3410000000a293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_52.3410000000a293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599692
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC402392.515421718680846No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT48190.3012454897567782No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG17990.11245914838606433No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG16440.10276978318326277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC45600.043.32098845
ATGCCGT51450.038.52646644
GCCCACG52700.037.69803618
CGAGACC52700.037.6126523
CCCACGA52900.037.5555119
CCACGAG52900.037.5555120
ACGAGAC53200.037.42831422
GTATGCC53100.037.3716942
TATGCCG53100.037.28694543
GACCTTG53500.037.13432726
CACGAGA53800.037.0945421
ATCTCGT53200.037.08997737
CGTATGC53600.037.0650541
CGAGCCC53600.037.0230715
TCGTATG53700.036.99602540
GAGCCCA53750.036.9197516
AGCCCAC53700.036.91222817
CTCGTAT53600.036.89714439
AGACCTT53800.036.8854425
CCGAGCC53950.036.86629514