FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_51.3410000000a286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_51.3410000000a286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626691
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC204521.257276274350814No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT29920.1839316747925697No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG21680.13327669483632726No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA18540.11397370490154554No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG18260.11225241917487709No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA16900.10389188850248757No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG16840.10352304156105861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC22500.043.09879345
ATGCCGT25650.037.80595844
TATGCCG27450.035.40884443
GTATGCC27800.034.8821242
CTCGTAT27900.034.7570939
TCGTATG28100.034.5097140
CGTATGC28400.034.14517241
GACTAGA28900.033.86583326
TATCTCG28550.033.8081636
AGACTAG28850.033.7685525
TCTCGTA29150.033.34383438
CCACGAG29200.033.2867420
GATCCTA29200.033.20968631
CGAGACT29300.033.17313423
CCTATCT29250.033.07599634
ACGAGAC29400.032.9837722
CACGAGA29550.032.8924821
CCCACGA29700.032.72635719
GAGACTA29700.032.72635724
GCCCACG30200.032.1845318