FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_50.3410000000a279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_50.3410000000a279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459044
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC41070.2814856851472608TruSeq Adapter, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5500.035.5908845
CGTATGC5650.033.84837341
TATGCCG5700.033.5526143
AGACTAC5100.033.0870825
GCCCACG5950.032.8980118
CCCACGA5900.032.79546419
TCGTATG5950.032.1417340
ATGCCGT6050.031.61154644
CGAGACT6200.031.20858623
GTATGCC6250.030.5989342
CTCGTAT6300.030.3560839
CCACGAG6650.029.09672420
CCGAGCC6800.028.45488514
TCCGAGC6950.028.16448213
AGCCCAC6950.028.16448217
ACGAGAC7000.027.64188822
GAGCCCA7650.026.17555416
CACGAGA7400.026.14773421
TACTTGC6550.025.76245529
CTCCGAG7650.025.29323212
Produced by FastQC (version 0.11.2)