Basic Statistics
Measure | Value |
---|---|
Filename | H23V3BCXX l01n01 kb2_2_49.3410000000a250.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 762080 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 37 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1778 | 0.23330883896703758 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC | 1313 | 0.17229162292672684 | RNA PCR Primer, Index 27 (95% over 21bp) |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 1269 | 0.16651795087129959 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1156 | 0.15169011127440687 | No Hit |
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1123 | 0.14735985723283643 | No Hit |
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1064 | 0.13961788788578627 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 881 | 0.11560466092798657 | No Hit |
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA | 816 | 0.10707537266428722 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATAGCG | 20 | 7.0328504E-4 | 44.998623 | 5 |
TATGCCG | 230 | 0.0 | 37.172775 | 43 |
CACGAGA | 245 | 0.0 | 35.81523 | 21 |
TATCTCG | 235 | 0.0 | 35.42445 | 36 |
CGAGACG | 225 | 0.0 | 34.99893 | 23 |
GAGACGA | 235 | 0.0 | 34.46703 | 24 |
ATCTCGT | 250 | 0.0 | 34.19895 | 37 |
CCCACGA | 255 | 0.0 | 33.528385 | 19 |
ACGAGAC | 265 | 0.0 | 33.112194 | 22 |
TGCCGTC | 260 | 0.0 | 32.883606 | 45 |
GTATGCC | 265 | 0.0 | 32.263165 | 42 |
TCTCGTA | 280 | 0.0 | 31.338324 | 38 |
ATGCCGT | 275 | 0.0 | 31.089958 | 44 |
CCACGAG | 285 | 0.0 | 29.999083 | 20 |
CCGGGTC | 45 | 3.4900462E-5 | 29.99908 | 21 |
CGTATGC | 295 | 0.0 | 28.982162 | 41 |
TCGTACG | 135 | 0.0 | 28.332466 | 10 |
TGCTATC | 285 | 0.0 | 27.630733 | 33 |
GCCCACG | 310 | 0.0 | 27.5798 | 18 |
ACGCGTT | 100 | 4.5474735E-11 | 26.999174 | 37 |