FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_49.3410000000a250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_49.3410000000a250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762080
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.23330883896703758No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC13130.17229162292672684RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA12690.16651795087129959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11560.15169011127440687No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11230.14735985723283643No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10640.13961788788578627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.11560466092798657No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA8160.10707537266428722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG207.0328504E-444.9986235
TATGCCG2300.037.17277543
CACGAGA2450.035.8152321
TATCTCG2350.035.4244536
CGAGACG2250.034.9989323
GAGACGA2350.034.4670324
ATCTCGT2500.034.1989537
CCCACGA2550.033.52838519
ACGAGAC2650.033.11219422
TGCCGTC2600.032.88360645
GTATGCC2650.032.26316542
TCTCGTA2800.031.33832438
ATGCCGT2750.031.08995844
CCACGAG2850.029.99908320
CCGGGTC453.4900462E-529.9990821
CGTATGC2950.028.98216241
TCGTACG1350.028.33246610
TGCTATC2850.027.63073333
GCCCACG3100.027.579818
ACGCGTT1004.5474735E-1126.99917437