FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_47.3410000000a236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_47.3410000000a236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC1016.380290587492103No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT120.7580543272267846No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC70.4421983575489577RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.3790271636133923TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT50.3158559696778269No Hit
TCCTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGA20.12634238787113075No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCGTATCTCGTATGCCGT20.12634238787113075No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC20.12634238787113075No Hit
GTATCAACGCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTG20.12634238787113075No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC205.0708407E-445.00000445
CCACGAG205.0708407E-445.00000420
CACGAGA205.0708407E-445.00000421
ACGAGAC205.0708407E-445.00000422
CGAGACT205.0708407E-445.00000423
AGACTAG205.0708407E-445.00000425
GAGACTA205.0708407E-445.00000424
AGCCCAC250.001523630836.017
ATCCTAT250.001523630836.032
GTATGCC250.001523630836.042
ATCTCGT250.001523630836.037
CCTATCT250.001523630836.034
ATCTCCG250.001523630836.010
GCCCACG250.001523630836.018
TCCTATC250.001523630836.033
TATGCCG250.001523630836.043
CATCTCC250.001523630836.09
GAGCCCA250.001523630836.016
CCCACGA250.001523630836.019
ACATCTC250.001523630836.08
Produced by FastQC (version 0.11.2)