FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_44.3410000000a20f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_44.3410000000a20f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC503.894080996884735No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT151.1682242990654206No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC60.46728971962616817RNA PCR Primer, Index 27 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG40.3115264797507788No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC30.23364485981308408TruSeq Adapter, Index 12 (95% over 21bp)
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCTGTCTCTT20.1557632398753894No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC20.1557632398753894No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC204.661695E-445.017
ATCCTAT204.661695E-445.032
GTATGCC204.661695E-445.042
ATCTCGT204.661695E-445.037
CCTATCT204.661695E-445.034
ATCTCCG204.661695E-445.010
CACATCT204.661695E-445.07
GCCCACG204.661695E-445.018
TCCTATC204.661695E-445.033
TGCCGTC204.661695E-445.045
CCACGAG204.661695E-445.020
TATGCCG204.661695E-445.043
CATCTCC204.661695E-445.09
GAGCCCA204.661695E-445.016
CACGAGA204.661695E-445.021
CCCACGA204.661695E-445.019
ACATCTC204.661695E-445.08
TTATACA204.661695E-445.02
TACACAT204.661695E-445.05
ACACATC204.661695E-445.06