##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_43.3410000000a1f1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1151 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.748913987836666 38.0 32.0 38.0 27.0 38.0 2 35.33970460469157 38.0 32.0 38.0 32.0 38.0 3 35.20590790616855 38.0 32.0 38.0 32.0 38.0 4 35.32927888792354 38.0 32.0 38.0 32.0 38.0 5 35.48566463944396 38.0 32.0 38.0 32.0 38.0 6 36.75325803649001 38.0 38.0 40.0 32.0 40.0 7 36.86446568201564 38.0 38.0 40.0 32.0 40.0 8 36.46394439617724 38.0 38.0 40.0 32.0 40.0 9 36.56125108601216 38.0 38.0 40.0 32.0 40.0 10 36.43006081668115 38.0 38.0 40.0 32.0 40.0 11 36.74456993918332 38.0 38.0 40.0 32.0 40.0 12 36.54735013032146 38.0 38.0 40.0 32.0 40.0 13 36.67593397046047 38.0 38.0 40.0 32.0 40.0 14 36.516941789748046 38.0 38.0 40.0 32.0 40.0 15 36.85230234578627 38.0 38.0 40.0 32.0 40.0 16 36.69765421372719 38.0 38.0 40.0 32.0 40.0 17 36.586446568201566 38.0 38.0 40.0 32.0 40.0 18 36.476107732406604 38.0 38.0 40.0 32.0 40.0 19 36.53518679409209 38.0 38.0 40.0 32.0 40.0 20 36.321459600347524 38.0 38.0 40.0 32.0 40.0 21 36.37271937445699 38.0 38.0 40.0 32.0 40.0 22 36.61598609904431 38.0 38.0 40.0 32.0 40.0 23 36.532580364900085 38.0 38.0 40.0 32.0 40.0 24 36.3640312771503 38.0 38.0 40.0 32.0 40.0 25 36.29452649869678 38.0 38.0 40.0 32.0 40.0 26 36.2128583840139 38.0 38.0 40.0 27.0 40.0 27 36.216333622936574 38.0 38.0 40.0 32.0 40.0 28 36.22675933970461 38.0 38.0 40.0 32.0 40.0 29 36.21372719374457 38.0 38.0 40.0 32.0 40.0 30 36.06081668114683 38.0 38.0 40.0 27.0 40.0 31 36.41702867072111 38.0 38.0 40.0 32.0 40.0 32 36.257167680278016 38.0 38.0 40.0 32.0 40.0 33 36.327541268462205 38.0 38.0 40.0 32.0 40.0 34 36.347523892267596 38.0 38.0 40.0 32.0 40.0 35 36.32319721980886 38.0 38.0 40.0 32.0 40.0 36 36.3388357949609 38.0 38.0 40.0 32.0 40.0 37 36.15291051259774 38.0 38.0 40.0 32.0 40.0 38 36.066029539530845 38.0 38.0 40.0 27.0 40.0 39 35.60034752389227 38.0 38.0 40.0 27.0 40.0 40 36.12945264986968 38.0 38.0 40.0 27.0 40.0 41 36.11468288444831 38.0 38.0 40.0 27.0 40.0 42 36.02953953084275 38.0 38.0 40.0 27.0 40.0 43 36.065160729800176 38.0 38.0 40.0 27.0 40.0 44 35.651607298001736 38.0 38.0 40.0 27.0 40.0 45 35.79756733275413 38.0 38.0 40.0 27.0 40.0 46 36.03301476976542 38.0 38.0 40.0 27.0 40.0 47 36.214596003475236 38.0 38.0 40.0 27.0 40.0 48 36.27280625543006 38.0 38.0 40.0 32.0 40.0 49 36.03996524761077 38.0 38.0 40.0 27.0 40.0 50 35.84535186794092 38.0 38.0 40.0 27.0 40.0 51 35.131190269331015 38.0 32.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.029100529100525 1101 2 -22.634920634920633 1101 3 -8.285714285714285 1101 4 -3.904761904761905 1101 5 -3.75661375661376 1101 6 0.9920634920634939 1101 7 1.8941798941798993 1101 8 -9.150793650793652 1101 9 -22.58730158730159 1101 10 -9.88095238095238 1101 11 -3.9788359788359813 1101 12 -3.2142857142857153 1101 13 -4.862433862433868 1101 14 -3.7883597883597844 1101 15 0.6084656084656004 1101 16 1.3333333333333357 1101 17 -4.436507936507937 1101 18 -8.275132275132272 1101 19 -23.420634920634917 1101 20 -22.708994708994716 1101 21 -23.111111111111114 1101 22 -9.73809523809524 1101 23 1.2698412698412724 1101 24 -22.81481481481481 1101 25 1.7354497354497411 1101 26 1.1931216931216895 1101 27 -9.301587301587304 1101 28 -3.9312169312169374 1101 29 -22.53174603174603 1101 30 -4.367724867724867 1101 31 1.018518518518519 1101 32 2.058201058201064 1101 33 1.6746031746031775 1101 34 2.8439153439153486 1101 35 -7.8915343915343925 1101 36 2.5608465608465565 1101 37 -22.26190476190476 1101 38 -3.981481481481474 1101 39 -22.46560846560846 1101 40 2.7804232804232782 1101 41 1.8703703703703667 1101 42 3.2010582010581956 1101 43 -8.838624338624342 1101 44 3.208994708994709 1101 45 2.7592592592592524 1101 46 1.640211640211632 1101 47 -3.452380952380949 1101 48 1.735449735449734 1101 49 3.037037037037038 1101 50 2.2962962962962905 1101 51 -7.383597883597879 2201 1 1.0291005291005249 2201 2 0.36507936507936734 2201 3 0.7142857142857153 2201 4 0.0952380952380949 2201 5 0.24338624338624015 2201 6 0.9920634920634939 2201 7 0.5608465608465636 2201 8 2.5158730158730123 2201 9 3.0793650793650755 2201 10 1.7857142857142847 2201 11 3.3544973544973544 2201 12 3.452380952380949 2201 13 2.470899470899468 2201 14 2.87830687830688 2201 15 1.941798941798936 2201 16 2.0 2201 17 0.896825396825399 2201 18 0.3915343915343925 2201 19 2.9126984126984183 2201 20 0.9576719576719483 2201 21 1.2222222222222214 2201 22 0.5952380952380949 2201 23 0.6031746031746081 2201 24 0.8518518518518547 2201 25 2.4021164021164054 2201 26 1.1931216931216895 2201 27 2.3650793650793602 2201 28 3.4021164021163983 2201 29 3.1349206349206327 2201 30 2.2989417989417973 2201 31 3.018518518518519 2201 32 2.058201058201064 2201 33 -5.99206349206349 2201 34 -4.822751322751319 2201 35 0.7751322751322718 2201 36 3.227513227513221 2201 37 4.0714285714285765 2201 38 0.6851851851851904 2201 39 -0.4656084656084616 2201 40 4.113756613756614 2201 41 2.537037037037031 2201 42 3.86772486772486 2201 43 2.828042328042322 2201 44 3.875661375661373 2201 45 3.4259259259259167 2201 46 2.9735449735449677 2201 47 2.547619047619051 2201 48 2.4021164021163983 2201 49 3.7037037037037024 2201 50 1.6296296296296262 2201 51 4.283068783068785 2202 1 3.029100529100525 2202 2 -0.6349206349206327 2202 3 -0.2857142857142847 2202 4 2.095238095238095 2202 5 2.24338624338624 2202 6 0.9920634920634939 2202 7 2.8941798941798993 2202 8 2.849206349206348 2202 9 -10.087301587301589 2202 10 -1.8809523809523796 2202 11 2.0211640211640187 2202 12 3.7857142857142847 2202 13 2.1375661375661323 2202 14 -0.7883597883597844 2202 15 1.6084656084656004 2202 16 -1.6666666666666643 2202 17 -0.43650793650793673 2202 18 3.724867724867728 2202 19 2.5793650793650826 2202 20 3.291005291005284 2202 21 -4.111111111111114 2202 22 1.2619047619047592 2202 23 1.2698412698412724 2202 24 2.1851851851851904 2202 25 -1.2645502645502589 2202 26 -11.30687830687831 2202 27 -10.801587301587304 2202 28 -10.431216931216937 2202 29 -10.031746031746032 2202 30 -2.867724867724867 2202 31 -3.481481481481481 2202 32 -10.441798941798936 2202 33 -2.8253968253968225 2202 34 -8.656084656084651 2202 35 -8.391534391534393 2202 36 -1.9391534391534435 2202 37 3.738095238095241 2202 38 2.018518518518526 2202 39 -0.4656084656084616 2202 40 -8.719576719576722 2202 41 -9.629629629629633 2202 42 -8.298941798941804 2202 43 -2.338624338624342 2202 44 -9.291005291005291 2202 45 0.7592592592592524 2202 46 -0.359788359788368 2202 47 -0.452380952380949 2202 48 -1.264550264550266 2202 49 -9.462962962962962 2202 50 -9.20370370370371 2202 51 -0.8835978835978793 1102 1 -14.970899470899475 1102 2 -3.1349206349206327 1102 3 -2.7857142857142847 1102 4 2.095238095238095 1102 5 2.24338624338624 1102 6 1.9920634920634939 1102 7 2.8941798941798993 1102 8 3.849206349206348 1102 9 2.4126984126984112 1102 10 2.1190476190476204 1102 11 3.0211640211640187 1102 12 2.7857142857142847 1102 13 1.1375661375661323 1102 14 2.2116402116402156 1102 15 0.6084656084656004 1102 16 2.3333333333333357 1102 17 1.5634920634920633 1102 18 3.724867724867728 1102 19 1.5793650793650826 1102 20 2.291005291005284 1102 21 3.8888888888888857 1102 22 3.261904761904759 1102 23 1.2698412698412724 1102 24 2.1851851851851904 1102 25 1.7354497354497411 1102 26 1.1931216931216895 1102 27 2.698412698412696 1102 28 2.0687830687830626 1102 29 3.4682539682539684 1102 30 1.632275132275133 1102 31 2.018518518518519 1102 32 4.058201058201064 1102 33 2.6746031746031775 1102 34 3.8439153439153486 1102 35 4.1084656084656075 1102 36 3.5608465608465565 1102 37 3.738095238095241 1102 38 -0.9814814814814738 1102 39 -0.4656084656084616 1102 40 -0.21957671957672176 1102 41 1.8703703703703667 1102 42 3.2010582010581956 1102 43 3.161375661375658 1102 44 4.208994708994709 1102 45 -8.740740740740748 1102 46 2.640211640211632 1102 47 3.547619047619051 1102 48 1.735449735449734 1102 49 5.037037037037038 1102 50 2.2962962962962905 1102 51 3.6164021164021207 2203 1 0.029100529100524852 2203 2 2.3650793650793673 2203 3 2.7142857142857153 2203 4 2.095238095238095 2203 5 2.24338624338624 2203 6 -1.0079365079365061 2203 7 2.8941798941798993 2203 8 1.849206349206348 2203 9 -3.0873015873015888 2203 10 1.1190476190476204 2203 11 2.0211640211640187 2203 12 2.7857142857142847 2203 13 1.1375661375661323 2203 14 2.2116402116402156 2203 15 0.6084656084656004 2203 16 2.3333333333333357 2203 17 -1.4365079365079367 2203 18 -12.775132275132272 2203 19 -1.4206349206349174 2203 20 2.291005291005284 2203 21 -1.1111111111111143 2203 22 1.2619047619047592 2203 23 1.2698412698412724 2203 24 -10.31481481481481 2203 25 1.7354497354497411 2203 26 2.1931216931216895 2203 27 1.698412698412696 2203 28 2.0687830687830626 2203 29 2.4682539682539684 2203 30 -6.867724867724867 2203 31 1.018518518518519 2203 32 -0.9417989417989361 2203 33 1.6746031746031775 2203 34 -9.656084656084651 2203 35 -8.391534391534393 2203 36 -1.9391534391534435 2203 37 -0.2619047619047592 2203 38 -0.9814814814814738 2203 39 2.5343915343915384 2203 40 2.7804232804232782 2203 41 1.8703703703703667 2203 42 3.2010582010581956 2203 43 2.161375661375658 2203 44 0.20899470899470884 2203 45 -0.24074074074074758 2203 46 1.640211640211632 2203 47 3.547619047619051 2203 48 -1.264550264550266 2203 49 3.037037037037038 2203 50 2.2962962962962905 2203 51 -0.8835978835978793 1103 1 0.029100529100524852 1103 2 -3.1349206349206327 1103 3 2.7142857142857153 1103 4 -0.9047619047619051 1103 5 -0.7566137566137598 1103 6 0.9920634920634939 1103 7 3.8941798941798993 1103 8 1.849206349206348 1103 9 3.4126984126984112 1103 10 3.1190476190476204 1103 11 3.0211640211640187 1103 12 2.7857142857142847 1103 13 2.1375661375661323 1103 14 3.2116402116402156 1103 15 0.6084656084656004 1103 16 1.3333333333333357 1103 17 3.5634920634920633 1103 18 3.724867724867728 1103 19 -0.42063492063491736 1103 20 2.291005291005284 1103 21 1.8888888888888857 1103 22 1.2619047619047592 1103 23 2.2698412698412724 1103 24 2.1851851851851904 1103 25 1.7354497354497411 1103 26 -1.8068783068783105 1103 27 2.698412698412696 1103 28 4.068783068783063 1103 29 3.4682539682539684 1103 30 2.632275132275133 1103 31 2.018518518518519 1103 32 3.058201058201064 1103 33 1.6746031746031775 1103 34 4.843915343915349 1103 35 4.1084656084656075 1103 36 2.5608465608465565 1103 37 2.738095238095241 1103 38 3.018518518518526 1103 39 2.5343915343915384 1103 40 2.7804232804232782 1103 41 2.8703703703703667 1103 42 3.2010582010581956 1103 43 3.161375661375658 1103 44 -2.291005291005291 1103 45 3.7592592592592524 1103 46 -0.359788359788368 1103 47 2.547619047619051 1103 48 2.735449735449734 1103 49 4.037037037037038 1103 50 2.2962962962962905 1103 51 3.6164021164021207 2204 1 3.029100529100525 2204 2 2.3650793650793673 2204 3 -0.2857142857142847 2204 4 -0.9047619047619051 2204 5 -3.75661375661376 2204 6 -2.007936507936506 2204 7 1.8941798941798993 2204 8 1.849206349206348 2204 9 2.4126984126984112 2204 10 1.1190476190476204 2204 11 -10.478835978835981 2204 12 -0.2142857142857153 2204 13 1.1375661375661323 2204 14 -0.7883597883597844 2204 15 0.6084656084656004 2204 16 1.3333333333333357 2204 17 2.5634920634920633 2204 18 -9.775132275132272 2204 19 2.5793650793650826 2204 20 -2.208994708994716 2204 21 -0.11111111111111427 2204 22 1.2619047619047592 2204 23 1.2698412698412724 2204 24 2.1851851851851904 2204 25 2.735449735449741 2204 26 2.1931216931216895 2204 27 2.698412698412696 2204 28 3.0687830687830626 2204 29 3.4682539682539684 2204 30 1.632275132275133 2204 31 -1.981481481481481 2204 32 -0.9417989417989361 2204 33 1.6746031746031775 2204 34 2.8439153439153486 2204 35 -2.8915343915343925 2204 36 -9.939153439153444 2204 37 -0.2619047619047592 2204 38 2.018518518518526 2204 39 -9.965608465608462 2204 40 -2.7195767195767218 2204 41 -10.629629629629633 2204 42 -8.298941798941804 2204 43 -3.338624338624342 2204 44 -1.2910052910052912 2204 45 -0.24074074074074758 2204 46 1.640211640211632 2204 47 -3.452380952380949 2204 48 -23.264550264550266 2204 49 -14.962962962962962 2204 50 -3.7037037037037095 2204 51 0.6164021164021207 2205 1 3.029100529100525 2205 2 2.3650793650793673 2205 3 -0.2857142857142847 2205 4 -0.9047619047619051 2205 5 -0.7566137566137598 2205 6 -5.007936507936506 2205 7 -1.1058201058201007 2205 8 -1.150793650793652 2205 9 2.4126984126984112 2205 10 -4.88095238095238 2205 11 -10.478835978835981 2205 12 2.7857142857142847 2205 13 -1.8624338624338677 2205 14 -3.7883597883597844 2205 15 -2.3915343915343996 2205 16 -4.666666666666664 2205 17 1.5634920634920633 2205 18 2.724867724867728 2205 19 -1.4206349206349174 2205 20 2.291005291005284 2205 21 -1.1111111111111143 2205 22 -1.7380952380952408 2205 23 -7.230158730158728 2205 24 -3.3148148148148096 2205 25 -3.764550264550259 2205 26 -4.3068783068783105 2205 27 -6.801587301587304 2205 28 -13.431216931216937 2205 29 -3.0317460317460316 2205 30 -3.867724867724867 2205 31 1.018518518518519 2205 32 2.058201058201064 2205 33 -1.3253968253968225 2205 34 -0.15608465608465139 2205 35 3.1084656084656075 2205 36 -3.4391534391534435 2205 37 -5.761904761904759 2205 38 -10.481481481481474 2205 39 2.5343915343915384 2205 40 2.7804232804232782 2205 41 -0.12962962962963331 2205 42 0.2010582010581956 2205 43 -13.338624338624342 2205 44 -9.291005291005291 2205 45 -2.7407407407407476 2205 46 -6.859788359788368 2205 47 -0.452380952380949 2205 48 1.735449735449734 2205 49 3.037037037037038 2205 50 -10.20370370370371 2205 51 4.616402116402121 1216 1 0.029100529100524852 1216 2 2.3650793650793673 1216 3 -0.2857142857142847 1216 4 -0.9047619047619051 1216 5 -3.75661375661376 1216 6 -4.507936507936506 1216 7 1.8941798941798993 1216 8 2.849206349206348 1216 9 0.41269841269841123 1216 10 1.1190476190476204 1216 11 -6.478835978835981 1216 12 -12.714285714285715 1216 13 1.1375661375661323 1216 14 2.2116402116402156 1216 15 0.6084656084656004 1216 16 1.3333333333333357 1216 17 -10.936507936507937 1216 18 -0.2751322751322718 1216 19 1.5793650793650826 1216 20 2.291005291005284 1216 21 -3.6111111111111143 1216 22 1.2619047619047592 1216 23 -1.7301587301587276 1216 24 -10.31481481481481 1216 25 -3.764550264550259 1216 26 1.1931216931216895 1216 27 1.698412698412696 1216 28 -3.9312169312169374 1216 29 2.4682539682539684 1216 30 1.632275132275133 1216 31 1.018518518518519 1216 32 -0.9417989417989361 1216 33 -1.3253968253968225 1216 34 -0.15608465608465139 1216 35 3.1084656084656075 1216 36 -0.4391534391534435 1216 37 -9.76190476190476 1216 38 -2.481481481481474 1216 39 2.5343915343915384 1216 40 2.7804232804232782 1216 41 1.8703703703703667 1216 42 3.2010582010581956 1216 43 2.161375661375658 1216 44 3.208994708994709 1216 45 2.7592592592592524 1216 46 1.640211640211632 1216 47 2.547619047619051 1216 48 -1.264550264550266 1216 49 -2.462962962962962 1216 50 2.2962962962962905 1216 51 -8.88359788359788 2206 1 3.029100529100525 2206 2 2.3650793650793673 2206 3 -0.2857142857142847 2206 4 -0.9047619047619051 2206 5 -0.7566137566137598 2206 6 0.9920634920634939 2206 7 1.8941798941798993 2206 8 1.849206349206348 2206 9 -0.5873015873015888 2206 10 -3.3809523809523796 2206 11 3.0211640211640187 2206 12 -2.7142857142857153 2206 13 1.1375661375661323 2206 14 -10.288359788359784 2206 15 -2.3915343915343996 2206 16 -7.166666666666664 2206 17 -6.936507936507937 2206 18 -5.775132275132272 2206 19 -1.4206349206349174 2206 20 2.291005291005284 2206 21 -3.6111111111111143 2206 22 -1.7380952380952408 2206 23 1.2698412698412724 2206 24 0.18518518518519045 2206 25 2.735449735449741 2206 26 -1.8068783068783105 2206 27 -1.301587301587304 2206 28 -13.431216931216937 2206 29 -3.5317460317460316 2206 30 -1.367724867724867 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39 2.5343915343915384 1208 40 2.7804232804232782 1208 41 -4.129629629629633 1208 42 -21.798941798941804 1208 43 -3.838624338624342 1208 44 -21.79100529100529 1208 45 2.7592592592592524 1208 46 1.640211640211632 1208 47 -3.452380952380949 1208 48 -4.264550264550266 1208 49 -2.962962962962962 1208 50 2.2962962962962905 1208 51 -21.38359788359788 1209 1 0.029100529100524852 1209 2 -0.6349206349206327 1209 3 -0.2857142857142847 1209 4 -0.9047619047619051 1209 5 2.24338624338624 1209 6 1.9920634920634939 1209 7 -1.1058201058201007 1209 8 -0.15079365079365203 1209 9 -10.087301587301589 1209 10 -3.3809523809523796 1209 11 -9.478835978835981 1209 12 -1.7142857142857153 1209 13 -3.3624338624338677 1209 14 3.2116402116402156 1209 15 -10.8915343915344 1209 16 -0.6666666666666643 1209 17 -10.936507936507937 1209 18 -0.2751322751322718 1209 19 2.5793650793650826 1209 20 2.291005291005284 1209 21 -9.611111111111114 1209 22 -1.7380952380952408 1209 23 2.2698412698412724 1209 24 0.18518518518519045 1209 25 -6.764550264550259 1209 26 -7.3068783068783105 1209 27 1.698412698412696 1209 28 2.0687830687830626 1209 29 2.4682539682539684 1209 30 -10.867724867724867 1209 31 1.018518518518519 1209 32 -10.441798941798936 1209 33 -2.8253968253968225 1209 34 3.8439153439153486 1209 35 3.1084656084656075 1209 36 -0.4391534391534435 1209 37 2.738095238095241 1209 38 2.018518518518526 1209 39 3.5343915343915384 1209 40 -0.21957671957672176 1209 41 1.8703703703703667 1209 42 1.2010582010581956 1209 43 -3.838624338624342 1209 44 0.20899470899470884 1209 45 3.7592592592592524 1209 46 -9.859788359788368 1209 47 3.547619047619051 1209 48 -1.264550264550266 1209 49 0.03703703703703809 1209 50 -3.2037037037037095 1209 51 3.6164021164021207 1214 1 -2.470899470899475 1214 2 -15.634920634920633 1214 3 -15.285714285714285 1214 4 -3.404761904761905 1214 5 -0.7566137566137598 1214 6 0.9920634920634939 1214 7 1.8941798941798993 1214 8 -1.150793650793652 1214 9 -0.5873015873015888 1214 10 -1.8809523809523796 1214 11 2.0211640211640187 1214 12 -1.7142857142857153 1214 13 -10.362433862433868 1214 14 0.21164021164021563 1214 15 0.6084656084656004 1214 16 -1.6666666666666643 1214 17 -1.4365079365079367 1214 18 2.724867724867728 1214 19 1.5793650793650826 1214 20 2.291005291005284 1214 21 -1.1111111111111143 1214 22 -1.7380952380952408 1214 23 1.2698412698412724 1214 24 2.1851851851851904 1214 25 1.7354497354497411 1214 26 -11.30687830687831 1214 27 -1.301587301587304 1214 28 -13.431216931216937 1214 29 2.4682539682539684 1214 30 -13.867724867724867 1214 31 -9.981481481481481 1214 32 -0.9417989417989361 1214 33 1.6746031746031775 1214 34 0.8439153439153486 1214 35 -12.391534391534393 1214 36 -2.9391534391534435 1214 37 2.738095238095241 1214 38 -0.9814814814814738 1214 39 2.5343915343915384 1214 40 -0.21957671957672176 1214 41 1.8703703703703667 1214 42 3.2010582010581956 1214 43 2.161375661375658 1214 44 -2.291005291005291 1214 45 -3.2407407407407476 1214 46 -3.859788359788368 1214 47 -2.952380952380949 1214 48 1.735449735449734 1214 49 -9.462962962962962 1214 50 2.2962962962962905 1214 51 0.6164021164021207 1215 1 3.029100529100525 1215 2 2.3650793650793673 1215 3 2.7142857142857153 1215 4 2.095238095238095 1215 5 2.24338624338624 1215 6 2.992063492063494 1215 7 3.8941798941798993 1215 8 3.849206349206348 1215 9 4.412698412698411 1215 10 3.1190476190476204 1215 11 4.021164021164019 1215 12 4.785714285714285 1215 13 3.1375661375661323 1215 14 4.211640211640216 1215 15 2.6084656084656004 1215 16 3.3333333333333357 1215 17 3.5634920634920633 1215 18 4.724867724867728 1215 19 3.5793650793650826 1215 20 4.291005291005284 1215 21 3.8888888888888857 1215 22 3.261904761904759 1215 23 3.2698412698412724 1215 24 2.1851851851851904 1215 25 3.735449735449741 1215 26 3.1931216931216895 1215 27 3.698412698412696 1215 28 2.0687830687830626 1215 29 4.468253968253968 1215 30 3.632275132275133 1215 31 3.018518518518519 1215 32 4.058201058201064 1215 33 3.6746031746031775 1215 34 4.843915343915349 1215 35 5.1084656084656075 1215 36 4.5608465608465565 1215 37 4.738095238095241 1215 38 4.018518518518526 1215 39 4.534391534391538 1215 40 4.780423280423278 1215 41 3.8703703703703667 1215 42 5.201058201058196 1215 43 4.161375661375658 1215 44 3.208994708994709 1215 45 2.7592592592592524 1215 46 1.640211640211632 1215 47 4.547619047619051 1215 48 3.735449735449734 1215 49 5.037037037037038 1215 50 4.2962962962962905 1215 51 5.616402116402121 1212 1 0.029100529100524852 1212 2 2.3650793650793673 1212 3 -0.2857142857142847 1212 4 2.095238095238095 1212 5 -0.7566137566137598 1212 6 -1.0079365079365061 1212 7 -9.6058201058201 1212 8 -2.650793650793652 1212 9 2.4126984126984112 1212 10 -4.38095238095238 1212 11 3.0211640211640187 1212 12 -8.714285714285715 1212 13 -4.362433862433868 1212 14 -2.2883597883597844 1212 15 -3.8915343915343996 1212 16 -10.166666666666664 1212 17 1.5634920634920633 1212 18 -2.775132275132272 1212 19 2.5793650793650826 1212 20 2.291005291005284 1212 21 1.8888888888888857 1212 22 -4.238095238095241 1212 23 -11.230158730158728 1212 24 -3.3148148148148096 1212 25 -2.764550264550259 1212 26 2.1931216931216895 1212 27 2.698412698412696 1212 28 4.068783068783063 1212 29 -0.5317460317460316 1212 30 2.632275132275133 1212 31 -11.481481481481481 1212 32 -2.441798941798936 1212 33 -0.32539682539682246 1212 34 -8.656084656084651 1212 35 -1.3915343915343925 1212 36 -1.9391534391534435 1212 37 -2.761904761904759 1212 38 -9.481481481481474 1212 39 2.5343915343915384 1212 40 2.7804232804232782 1212 41 3.8703703703703667 1212 42 4.201058201058196 1212 43 0.16137566137565784 1212 44 -2.291005291005291 1212 45 -9.740740740740748 1212 46 -10.859788359788368 1212 47 -1.952380952380949 1212 48 -2.764550264550266 1212 49 -2.462962962962962 1212 50 -9.20370370370371 1212 51 -0.8835978835978793 1213 1 3.029100529100525 1213 2 2.3650793650793673 1213 3 2.7142857142857153 1213 4 2.095238095238095 1213 5 2.24338624338624 1213 6 2.992063492063494 1213 7 3.8941798941798993 1213 8 3.849206349206348 1213 9 4.412698412698411 1213 10 3.1190476190476204 1213 11 4.021164021164019 1213 12 4.785714285714285 1213 13 3.1375661375661323 1213 14 4.211640211640216 1213 15 2.6084656084656004 1213 16 3.3333333333333357 1213 17 3.5634920634920633 1213 18 4.724867724867728 1213 19 3.5793650793650826 1213 20 2.291005291005284 1213 21 1.8888888888888857 1213 22 1.2619047619047592 1213 23 1.2698412698412724 1213 24 2.1851851851851904 1213 25 1.7354497354497411 1213 26 3.1931216931216895 1213 27 3.698412698412696 1213 28 2.0687830687830626 1213 29 4.468253968253968 1213 30 1.632275132275133 1213 31 3.018518518518519 1213 32 2.058201058201064 1213 33 1.6746031746031775 1213 34 2.8439153439153486 1213 35 5.1084656084656075 1213 36 2.5608465608465565 1213 37 2.738095238095241 1213 38 4.018518518518526 1213 39 2.5343915343915384 1213 40 4.780423280423278 1213 41 1.8703703703703667 1213 42 5.201058201058196 1213 43 2.161375661375658 1213 44 3.208994708994709 1213 45 4.759259259259252 1213 46 3.640211640211632 1213 47 4.547619047619051 1213 48 1.735449735449734 1213 49 3.037037037037038 1213 50 2.2962962962962905 1213 51 3.6164021164021207 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 2.0 23 2.0 24 4.0 25 7.0 26 7.0 27 15.0 28 26.0 29 24.0 30 34.0 31 32.0 32 50.0 33 53.0 34 73.0 35 87.0 36 109.0 37 132.0 38 238.0 39 255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.23344947735192 20.557491289198605 13.240418118466899 31.968641114982578 2 12.250217202432667 21.807124239791488 47.4370112945265 18.505647263249347 3 14.335360556038227 25.021720243266728 38.40139009556907 22.241529105125977 4 11.989574283231972 24.06602953953084 39.61772371850565 24.326672458731537 5 11.03388357949609 37.011294526498695 41.09470026064292 10.860121633362294 6 23.71850564726325 45.96003475238923 17.984361424847958 12.337098175499566 7 20.938314509122502 34.926151172893135 24.152910512597742 19.98262380538662 8 21.894005212858385 41.18158123370982 21.980886185925282 14.943527367506515 9 22.589052997393573 18.071242397914858 21.285838401390095 38.05386620330148 10 12.59774109470026 35.79496090356212 33.10165073848827 18.505647263249347 11 27.88879235447437 26.411815812337096 29.800173761946137 15.8992180712424 12 16.420503909643788 26.151172893136405 34.404865334491745 23.023457862728065 13 23.370981754995658 23.63162467419635 32.49348392701998 20.50390964378801 14 18.592528236316248 19.808861859252826 29.105125977410946 32.49348392701998 15 19.46133796698523 30.668983492615116 25.28236316246742 24.587315377932235 16 25.36924413553432 30.755864465682016 24.76107732406603 19.113814074717634 17 19.721980886185925 33.70981754995656 28.757602085143354 17.810599478714163 18 27.975673327541266 28.58384013900956 25.716768027801912 17.723718505647263 19 21.63336229365769 26.84622067767159 26.585577758470897 24.934839270199827 20 19.635099913119028 27.628149435273674 27.19374456993918 25.543006081668114 21 17.549956559513465 28.931364031277152 26.672458731537795 26.84622067767159 22 17.37619461337967 37.35881841876629 26.585577758470897 18.679409209383145 23 22.15464813205908 25.716768027801912 27.367506516072982 24.76107732406603 24 26.151172893136405 28.149435273675067 25.977410947002603 19.721980886185925 25 21.112076455256297 31.7115551694179 26.93310165073849 20.243266724587315 26 24.413553431798434 29.452649869678538 27.975673327541266 18.158123370981755 27 20.851433536055602 34.926151172893135 24.23979148566464 19.98262380538662 28 19.113814074717634 28.062554300608166 26.672458731537795 26.151172893136405 29 18.94005212858384 30.06081668114683 31.4509122502172 19.548218940052127 30 18.505647263249347 34.75238922675934 27.367506516072982 19.374456993918333 31 25.19548218940052 28.149435273675067 26.411815812337096 20.243266724587315 32 18.679409209383145 33.79669852302346 27.106863596872284 20.417028670721113 33 18.766290182450042 28.149435273675067 34.231103388357944 18.853171155516943 34 18.94005212858384 25.36924413553432 27.88879235447437 27.801911381407475 35 20.59079061685491 23.979148566463945 26.585577758470897 28.84448305821025 36 17.636837532580365 26.672458731537795 33.622936576889664 22.06776715899218 37 17.810599478714163 31.364031277150307 29.626411815812336 21.198957428323197 38 17.463075586446568 24.500434404865334 36.8375325803649 21.198957428323197 39 19.721980886185925 23.11033883579496 29.018245004344053 28.149435273675067 40 17.89748045178106 24.23979148566464 35.53431798436142 22.328410078192874 41 20.938314509122502 24.76107732406603 28.149435273675067 26.151172893136405 42 25.62988705473501 22.936576889661165 30.14769765421373 21.285838401390095 43 18.766290182450042 23.370981754995658 34.926151172893135 22.936576889661165 44 15.291051259774111 30.929626411815814 29.973935708079928 23.80538662033015 45 16.333622936576887 22.502172024326672 37.706342311033886 23.457862728062555 46 21.459600347523892 25.62988705473501 29.53953084274544 23.370981754995658 47 15.030408340573414 23.457862728062555 33.70981754995656 27.801911381407475 48 15.291051259774111 24.500434404865334 29.973935708079928 30.234578627280627 49 21.372719374456995 23.892267593397047 30.06081668114683 24.674196350999132 50 12.337098175499566 25.28236316246742 38.31450912250217 24.06602953953084 51 15.551694178974804 25.62988705473501 28.149435273675067 30.668983492615116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 1.0 18 1.0 19 1.0 20 1.0 21 0.5 22 0.0 23 0.5 24 1.0 25 3.5 26 7.5 27 9.0 28 18.0 29 27.0 30 33.5 31 40.0 32 57.5 33 75.0 34 83.0 35 91.0 36 99.0 37 107.0 38 113.5 39 120.0 40 131.5 41 143.0 42 130.0 43 117.0 44 95.0 45 73.0 46 69.5 47 66.0 48 63.5 49 61.0 50 87.5 51 114.0 52 71.0 53 28.0 54 21.0 55 14.0 56 14.5 57 15.0 58 16.0 59 17.0 60 13.5 61 10.0 62 6.5 63 3.0 64 2.5 65 2.0 66 3.0 67 4.0 68 2.5 69 1.0 70 2.0 71 3.0 72 1.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26064291920069504 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1151.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.57080799304953 #Duplication Level Percentage of deduplicated Percentage of total 1 99.44289693593315 93.04952215464813 2 0.2785515320334262 0.5212858384013901 3 0.0 0.0 4 0.0 0.0 5 0.09285051067780872 0.4344048653344918 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.09285051067780872 0.7819287576020852 >10 0.0 0.0 >50 0.09285051067780872 5.2128583840139004 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 60 5.2128583840139004 No Hit TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT 9 0.7819287576020852 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 5 0.4344048653344918 RNA PCR Primer, Index 27 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTTC 2 0.1737619461337967 TruSeq Adapter, Index 27 (95% over 22bp) TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT 2 0.1737619461337967 RNA PCR Primer, Index 27 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG 2 0.1737619461337967 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.26064291920069504 30 0.0 0.0 0.43440486533449174 31 0.0 0.0 1.3032145960034753 32 0.0 0.0 2.519548218940052 33 0.0 0.0 3.9965247610773242 34 0.0 0.0 5.039096437880104 35 0.0 0.0 5.907906168549088 36 0.0 0.0 6.776715899218071 37 0.0 0.0 8.166811468288445 38 0.0 0.0 9.383145091225021 39 0.0 0.0 11.902693310165073 >>END_MODULE >>Kmer Content pass >>END_MODULE