FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_41.3410000000a1d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_41.3410000000a1d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC927.198748043818466No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT151.1737089201877933No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC40.3129890453834116No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC40.3129890453834116RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC30.2347417840375587TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG30.2347417840375587No Hit
CTCATAGATCTGTGATTACACTTTGTGCTACTATAGATTGGTGTTGATCTG20.1564945226917058No Hit
TTATATGGTAATGCAATCTATCATGCATCCCTACGCGAAGATAAGCATGTA20.1564945226917058No Hit
CTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGGAAGGGAAAT20.1564945226917058No Hit
GAATATTTGCTACTACCACCAAGATCTGCACCTGTCTCTTATACACATCTC20.1564945226917058No Hit
CCGCGGAAGCAGCTCGAGCAGTCCGCTGACAGCCGACGGGTTCGGAACTGG20.1564945226917058No Hit
ATATAAATGAAAACGCGTTCTTCGATTTTTTAACCAATTCAACTCTACAAT20.1564945226917058No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)