FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_40.3410000000a1ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_40.3410000000a1ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC503.819709702062643No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT80.6111535523300229No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC80.6111535523300229RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC50.38197097020626436No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC30.2291825821237586TruSeq Adapter, Index 12 (95% over 21bp)
GTACTTGTTCACTATCGGTCTTTCAGGAGTATTTAGCCTGTCTCTTATACA20.15278838808250572No Hit
CTTCCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGAC20.15278838808250572No Hit
GGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGGCTTTACCCGATA20.15278838808250572No Hit
GACAGGTATCGTAAATTTATTTACGAGGCAAACCGGGAGAAGTGAAACATC20.15278838808250572No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTCTGTCTCTT20.15278838808250572No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)