##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_40.3410000000a1ca.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1309 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.97631779984721 38.0 32.0 38.0 32.0 38.0 2 35.31856378915202 38.0 32.0 38.0 32.0 38.0 3 35.180290297937354 38.0 32.0 38.0 32.0 38.0 4 35.618792971734145 38.0 32.0 38.0 32.0 38.0 5 35.54621848739496 38.0 32.0 38.0 32.0 38.0 6 36.97173414820474 38.0 38.0 40.0 32.0 40.0 7 36.82276546982429 38.0 38.0 40.0 32.0 40.0 8 36.632543926661576 38.0 38.0 40.0 32.0 40.0 9 36.796027501909855 38.0 38.0 40.0 32.0 40.0 10 37.075630252100844 38.0 38.0 40.0 32.0 40.0 11 36.87318563789152 38.0 38.0 40.0 32.0 40.0 12 36.83804430863255 38.0 38.0 40.0 32.0 40.0 13 36.71734148204737 38.0 38.0 40.0 32.0 40.0 14 36.870893812070285 38.0 38.0 40.0 32.0 40.0 15 36.94805194805195 38.0 38.0 40.0 32.0 40.0 16 36.65087853323148 38.0 38.0 40.0 32.0 40.0 17 36.74255156608098 38.0 38.0 40.0 32.0 40.0 18 36.63865546218487 38.0 38.0 40.0 32.0 40.0 19 36.83346065699007 38.0 38.0 40.0 32.0 40.0 20 36.705882352941174 38.0 38.0 40.0 32.0 40.0 21 36.80213903743316 38.0 38.0 40.0 32.0 40.0 22 36.518716577540104 38.0 38.0 40.0 32.0 40.0 23 37.0183346065699 38.0 38.0 40.0 32.0 40.0 24 36.65393430099312 38.0 38.0 40.0 32.0 40.0 25 36.48433919022154 38.0 38.0 40.0 32.0 40.0 26 36.596638655462186 38.0 38.0 40.0 32.0 40.0 27 36.603514132925895 38.0 38.0 40.0 32.0 40.0 28 36.58212375859435 38.0 38.0 40.0 32.0 40.0 29 36.583651642475175 38.0 38.0 40.0 32.0 40.0 30 36.3292589763178 38.0 38.0 40.0 32.0 40.0 31 36.245225362872425 38.0 38.0 40.0 27.0 40.0 32 36.46524064171123 38.0 38.0 40.0 32.0 40.0 33 36.17494270435447 38.0 38.0 40.0 27.0 40.0 34 36.22077922077922 38.0 38.0 40.0 27.0 40.0 35 36.21008403361345 38.0 38.0 40.0 27.0 40.0 36 36.44843391902216 38.0 38.0 40.0 32.0 40.0 37 36.236822001527884 38.0 38.0 40.0 32.0 40.0 38 36.28418640183346 38.0 38.0 40.0 32.0 40.0 39 36.32849503437739 38.0 38.0 40.0 27.0 40.0 40 36.19938884644767 38.0 38.0 40.0 32.0 40.0 41 36.27654698242934 38.0 38.0 40.0 32.0 40.0 42 36.049656226126814 38.0 38.0 40.0 27.0 40.0 43 36.15049656226127 38.0 38.0 40.0 27.0 40.0 44 35.97631779984721 38.0 38.0 40.0 27.0 40.0 45 36.05347593582888 38.0 38.0 40.0 27.0 40.0 46 36.14209320091673 38.0 38.0 40.0 27.0 40.0 47 36.3048128342246 38.0 38.0 40.0 27.0 40.0 48 36.05194805194805 38.0 38.0 40.0 27.0 40.0 49 36.013750954927424 38.0 38.0 40.0 27.0 40.0 50 36.15889992360581 38.0 38.0 40.0 27.0 40.0 51 35.51336898395722 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.1746031746031775 1101 2 -0.3478835978836017 1101 3 -3.526455026455025 1101 4 1.962962962962969 1101 5 -0.5502645502645578 1101 6 -0.6111111111111143 1101 7 -10.525132275132279 1101 8 -0.32671957671957585 1101 9 1.386243386243386 1101 10 -2.3518518518518476 1101 11 0.8571428571428612 1101 12 -1.2910052910052912 1101 13 1.363756613756621 1101 14 -11.353174603174594 1101 15 -3.1785714285714306 1101 16 -2.7552910052910065 1101 17 -0.8597883597883609 1101 18 -0.7275132275132279 1101 19 2.775132275132279 1101 20 2.0423280423280445 1101 21 1.5343915343915313 1101 22 1.3425925925925952 1101 23 -1.948412698412696 1101 24 3.1785714285714306 1101 25 1.8439153439153415 1101 26 1.8994708994708986 1101 27 2.481481481481474 1101 28 -10.216931216931215 1101 29 1.1746031746031846 1101 30 1.7658730158730265 1101 31 -0.009259259259259522 1101 32 -0.5330687830687779 1101 33 -9.318783068783063 1101 34 -1.051587301587297 1101 35 -1.2037037037037095 1101 36 2.2552910052909994 1101 37 0.24338624338624015 1101 38 -9.958994708994716 1101 39 -3.231481481481474 1101 40 -9.080687830687829 1101 41 2.477513227513221 1101 42 -9.87830687830688 1101 43 -8.95370370370371 1101 44 -3.1891534391534435 1101 45 -0.19973544973544222 1101 46 -12.410052910052912 1101 47 2.8293650793650826 1101 48 -12.107142857142854 1101 49 -10.029100529100525 1101 50 -9.37566137566138 1101 51 -0.1679894179894177 2201 1 3.1746031746031775 2201 2 2.6521164021163983 2201 3 2.473544973544975 2201 4 1.962962962962969 2201 5 2.449735449735442 2201 6 3.3888888888888857 2201 7 2.974867724867721 2201 8 2.3399470899470884 2201 9 2.7195767195767218 2201 10 1.981481481481488 2201 11 2.190476190476197 2201 12 2.0423280423280445 2201 13 2.0304232804232853 2201 14 1.8134920634920704 2201 15 3.3214285714285694 2201 16 3.0780423280423292 2201 17 2.473544973544975 2201 18 3.272486772486772 2201 19 2.441798941798943 2201 20 3.3756613756613802 2201 21 2.2010582010581956 2201 22 2.0092592592592595 2201 23 3.051587301587304 2201 24 3.5119047619047663 2201 25 3.177248677248677 2201 26 3.8994708994708986 2201 27 2.8148148148148096 2201 28 4.283068783068785 2201 29 3.1746031746031846 2201 30 3.7658730158730265 2201 31 3.9907407407407405 2201 32 2.800264550264558 2201 33 5.181216931216937 2201 34 3.948412698412703 2201 35 3.129629629629626 2201 36 3.2552910052909994 2201 37 3.576719576719576 2201 38 3.8743386243386198 2201 39 2.9351851851851904 2201 40 3.752645502645507 2201 41 3.144179894179885 2201 42 2.2883597883597844 2201 43 3.212962962962955 2201 44 2.977513227513221 2201 45 3.466931216931222 2201 46 5.089947089947088 2201 47 1.496031746031747 2201 48 2.7261904761904816 2201 49 -4.529100529100525 2201 50 2.4576719576719555 2201 51 4.165343915343918 2202 1 -2.4920634920634868 2202 2 -5.681216931216934 2202 3 -5.859788359788357 2202 4 -0.037037037037030984 2202 5 0.4497354497354422 2202 6 2.05555555555555 2202 7 1.6415343915343854 2202 8 3.00661375661376 2202 9 2.0529100529100504 2202 10 2.6481481481481524 2202 11 0.1904761904761969 2202 12 0.042328042328044546 2202 13 2.6970899470899568 2202 14 -0.18650793650792963 2202 15 -3.6785714285714306 2202 16 0.41137566137565784 2202 17 -0.19312169312169658 2202 18 -7.06084656084656 2202 19 -0.2248677248677211 2202 20 -5.624338624338623 2202 21 0.2010582010581956 2202 22 0.6759259259259309 2202 23 -0.28174603174603163 2202 24 -0.8214285714285694 2202 25 -5.822751322751326 2202 26 -1.7671957671957657 2202 27 2.148148148148138 2202 28 -5.383597883597883 2202 29 -1.8253968253968154 2202 30 -6.567460317460306 2202 31 3.324074074074076 2202 32 -1.533068783068778 2202 33 -4.4854497354497305 2202 34 -5.051587301587297 2202 35 -1.2037037037037095 2202 36 -1.7447089947090006 2202 37 1.5767195767195759 2202 38 -4.458994708994716 2202 39 0.26851851851852615 2202 40 2.419312169312171 2202 41 0.4775132275132208 2202 42 -0.3783068783068799 2202 43 1.2129629629629548 2202 44 0.3108465608465565 2202 45 -5.533068783068774 2202 46 0.08994708994708844 2202 47 2.8293650793650826 2202 48 1.392857142857146 2202 49 3.1375661375661394 2202 50 -4.542328042328048 2202 51 3.4986772486772466 1102 1 1.1746031746031775 1102 2 0.6521164021163983 1102 3 -1.5264550264550252 1102 4 -2.037037037037031 1102 5 0.4497354497354422 1102 6 2.05555555555555 1102 7 1.6415343915343854 1102 8 1.6732804232804241 1102 9 1.386243386243386 1102 10 0.6481481481481524 1102 11 -0.4761904761904745 1102 12 -2.291005291005291 1102 13 2.0304232804232853 1102 14 -0.18650793650792963 1102 15 1.9880952380952337 1102 16 2.411375661375658 1102 17 -1.8597883597883609 1102 18 -0.06084656084656359 1102 19 -7.891534391534389 1102 20 0.7089947089947088 1102 21 2.2010582010581956 1102 22 -1.6574074074074048 1102 23 1.7182539682539684 1102 24 2.845238095238095 1102 25 3.177248677248677 1102 26 3.2328042328042343 1102 27 1.4814814814814738 1102 28 0.9497354497354493 1102 29 1.841269841269849 1102 30 -1.2341269841269735 1102 31 2.6574074074074048 1102 32 2.1335978835978864 1102 33 1.8478835978836017 1102 34 -0.05158730158729696 1102 35 1.7962962962962905 1102 36 1.2552910052909994 1102 37 2.24338624338624 1102 38 0.541005291005284 1102 39 2.268518518518526 1102 40 -5.247354497354497 1102 41 3.144179894179885 1102 42 2.2883597883597844 1102 43 1.2129629629629548 1102 44 0.3108465608465565 1102 45 2.1335978835978935 1102 46 4.423280423280424 1102 47 2.8293650793650826 1102 48 2.05952380952381 1102 49 3.1375661375661394 1102 50 3.791005291005284 1102 51 3.4986772486772466 2203 1 3.1746031746031775 2203 2 2.6521164021163983 2203 3 2.473544973544975 2203 4 1.962962962962969 2203 5 2.449735449735442 2203 6 3.3888888888888857 2203 7 2.974867724867721 2203 8 3.673280423280424 2203 9 3.386243386243386 2203 10 2.6481481481481524 2203 11 2.857142857142861 2203 12 2.708994708994709 2203 13 3.363756613756621 2203 14 3.146825396825406 2203 15 3.3214285714285694 2203 16 3.7447089947089935 2203 17 3.140211640211639 2203 18 3.272486772486772 2203 19 3.775132275132279 2203 20 4.0423280423280445 2203 21 3.5343915343915313 2203 22 3.3425925925925952 2203 23 3.051587301587304 2203 24 4.178571428571431 2203 25 2.8439153439153415 2203 26 2.8994708994708986 2203 27 2.481481481481474 2203 28 3.283068783068785 2203 29 3.1746031746031846 2203 30 3.7658730158730265 2203 31 -2.5092592592592595 2203 32 -3.033068783068778 2203 33 4.181216931216937 2203 34 -2.551587301587297 2203 35 2.7962962962962905 2203 36 -0.7447089947090006 2203 37 -9.25661375661376 2203 38 3.541005291005284 2203 39 0.26851851851852615 2203 40 3.419312169312171 2203 41 3.477513227513221 2203 42 2.62169312169312 2203 43 3.5462962962962905 2203 44 4.3108465608465565 2203 45 4.800264550264558 2203 46 5.089947089947088 2203 47 4.829365079365083 2203 48 5.392857142857146 2203 49 4.470899470899475 2203 50 5.12433862433862 2203 51 4.832010582010582 1103 1 0.17460317460317754 1103 2 -3.3478835978836017 1103 3 -0.5264550264550252 1103 4 -1.037037037037031 1103 5 2.449735449735442 1103 6 2.3888888888888857 1103 7 2.974867724867721 1103 8 -1.3267195767195759 1103 9 -1.613756613756614 1103 10 -1.3518518518518476 1103 11 -4.642857142857139 1103 12 0.7089947089947088 1103 13 -11.136243386243379 1103 14 1.146825396825406 1103 15 -16.67857142857143 1103 16 -10.755291005291006 1103 17 -9.85978835978836 1103 18 -4.727513227513228 1103 19 -3.724867724867721 1103 20 -22.957671957671955 1103 21 -13.965608465608469 1103 22 -14.157407407407405 1103 23 1.051587301587304 1103 24 -10.32142857142857 1103 25 -10.656084656084658 1103 26 -6.600529100529101 1103 27 -16.518518518518526 1103 28 -10.216931216931215 1103 29 -4.325396825396815 1103 30 -3.7341269841269735 1103 31 1.9907407407407405 1103 32 -11.033068783068778 1103 33 -21.818783068783063 1103 34 -3.551587301587297 1103 35 -13.70370370370371 1103 36 -1.7447089947090006 1103 37 -10.25661375661376 1103 38 -12.958994708994716 1103 39 -3.231481481481474 1103 40 -6.080687830687829 1103 41 -3.022486772486779 1103 42 1.62169312169312 1103 43 -9.95370370370371 1103 44 -10.189153439153444 1103 45 -0.19973544973544222 1103 46 3.0899470899470884 1103 47 -0.17063492063491736 1103 48 0.3928571428571459 1103 49 -22.529100529100525 1103 50 -5.37566137566138 1103 51 -5.667989417989418 2204 1 -9.325396825396822 2204 2 2.6521164021163983 2204 3 2.473544973544975 2204 4 1.962962962962969 2204 5 2.449735449735442 2204 6 3.3888888888888857 2204 7 2.974867724867721 2204 8 1.6732804232804241 2204 9 2.386243386243386 2204 10 1.6481481481481524 2204 11 2.857142857142861 2204 12 1.7089947089947088 2204 13 2.363756613756621 2204 14 2.146825396825406 2204 15 2.3214285714285694 2204 16 3.7447089947089935 2204 17 3.140211640211639 2204 18 1.272486772486772 2204 19 3.775132275132279 2204 20 4.0423280423280445 2204 21 2.5343915343915313 2204 22 2.3425925925925952 2204 23 3.051587301587304 2204 24 3.1785714285714306 2204 25 2.8439153439153415 2204 26 3.8994708994708986 2204 27 3.481481481481474 2204 28 3.283068783068785 2204 29 2.1746031746031846 2204 30 2.7658730158730265 2204 31 3.9907407407407405 2204 32 3.466931216931222 2204 33 5.181216931216937 2204 34 3.948412698412703 2204 35 3.7962962962962905 2204 36 3.2552910052909994 2204 37 3.24338624338624 2204 38 3.541005291005284 2204 39 2.268518518518526 2204 40 4.419312169312171 2204 41 3.477513227513221 2204 42 2.62169312169312 2204 43 2.5462962962962905 2204 44 3.3108465608465565 2204 45 2.800264550264558 2204 46 4.089947089947088 2204 47 3.8293650793650826 2204 48 4.392857142857146 2204 49 4.470899470899475 2204 50 5.12433862433862 2204 51 3.8320105820105823 2205 1 3.1746031746031775 2205 2 2.6521164021163983 2205 3 -0.5264550264550252 2205 4 1.962962962962969 2205 5 2.449735449735442 2205 6 3.3888888888888857 2205 7 2.974867724867721 2205 8 2.673280423280424 2205 9 2.386243386243386 2205 10 2.6481481481481524 2205 11 2.857142857142861 2205 12 2.708994708994709 2205 13 3.363756613756621 2205 14 3.146825396825406 2205 15 2.3214285714285694 2205 16 2.7447089947089935 2205 17 2.140211640211639 2205 18 2.272486772486772 2205 19 2.775132275132279 2205 20 3.0423280423280445 2205 21 3.5343915343915313 2205 22 3.3425925925925952 2205 23 3.051587301587304 2205 24 3.1785714285714306 2205 25 2.8439153439153415 2205 26 2.8994708994708986 2205 27 2.481481481481474 2205 28 4.283068783068785 2205 29 3.1746031746031846 2205 30 3.7658730158730265 2205 31 2.9907407407407405 2205 32 2.466931216931222 2205 33 4.181216931216937 2205 34 2.948412698412703 2205 35 2.7962962962962905 2205 36 2.2552910052909994 2205 37 3.24338624338624 2205 38 3.541005291005284 2205 39 4.268518518518526 2205 40 4.419312169312171 2205 41 4.477513227513221 2205 42 3.62169312169312 2205 43 3.5462962962962905 2205 44 4.3108465608465565 2205 45 4.800264550264558 2205 46 5.089947089947088 2205 47 3.8293650793650826 2205 48 4.392857142857146 2205 49 3.470899470899475 2205 50 5.12433862433862 2205 51 4.832010582010582 1216 1 3.1746031746031775 1216 2 2.6521164021163983 1216 3 -0.5264550264550252 1216 4 1.962962962962969 1216 5 2.449735449735442 1216 6 3.3888888888888857 1216 7 2.974867724867721 1216 8 2.673280423280424 1216 9 2.386243386243386 1216 10 2.6481481481481524 1216 11 2.857142857142861 1216 12 2.708994708994709 1216 13 2.363756613756621 1216 14 2.146825396825406 1216 15 2.3214285714285694 1216 16 2.7447089947089935 1216 17 3.140211640211639 1216 18 3.272486772486772 1216 19 2.775132275132279 1216 20 3.0423280423280445 1216 21 3.5343915343915313 1216 22 3.3425925925925952 1216 23 3.051587301587304 1216 24 3.1785714285714306 1216 25 3.8439153439153415 1216 26 3.8994708994708986 1216 27 3.481481481481474 1216 28 3.283068783068785 1216 29 2.1746031746031846 1216 30 3.7658730158730265 1216 31 3.9907407407407405 1216 32 3.466931216931222 1216 33 4.181216931216937 1216 34 3.948412698412703 1216 35 3.7962962962962905 1216 36 3.2552910052909994 1216 37 3.24338624338624 1216 38 3.541005291005284 1216 39 4.268518518518526 1216 40 4.419312169312171 1216 41 4.477513227513221 1216 42 2.62169312169312 1216 43 3.5462962962962905 1216 44 3.3108465608465565 1216 45 3.800264550264558 1216 46 4.089947089947088 1216 47 3.8293650793650826 1216 48 4.392857142857146 1216 49 3.470899470899475 1216 50 1.1243386243386198 1216 51 0.8320105820105823 2206 1 -7.158730158730155 2206 2 -1.3478835978836017 2206 3 0.4735449735449748 2206 4 -2.037037037037031 2206 5 2.449735449735442 2206 6 2.05555555555555 2206 7 1.6415343915343854 2206 8 0.33994708994708844 2206 9 0.052910052910050354 2206 10 -3.018518518518512 2206 11 1.5238095238095255 2206 12 0.7089947089947088 2206 13 2.6970899470899568 2206 14 2.4801587301587418 2206 15 -0.011904761904766303 2206 16 1.0780423280423292 2206 17 -0.19312169312169658 2206 18 2.605820105820108 2206 19 3.1084656084656146 2206 20 -5.624338624338623 2206 21 2.2010582010581956 2206 22 2.0092592592592595 2206 23 -6.615079365079364 2206 24 0.8452380952380949 2206 25 2.510582010582006 2206 26 2.566137566137563 2206 27 2.148148148148138 2206 28 0.9497354497354493 2206 29 1.841269841269849 2206 30 2.4325396825396908 2206 31 3.324074074074076 2206 32 0.8002645502645578 2206 33 4.514550264550273 2206 34 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-3.1706349206349174 1208 48 -21.607142857142854 1208 49 -3.529100529100525 1208 50 3.1243386243386198 1208 51 2.8320105820105823 1209 1 1.1746031746031775 1209 2 0.6521164021163983 1209 3 -3.1931216931216895 1209 4 -0.037037037037030984 1209 5 2.449735449735442 1209 6 -8.277777777777782 1209 7 -0.3584656084656146 1209 8 -5.993386243386244 1209 9 2.7195767195767218 1209 10 1.3148148148148167 1209 11 -0.4761904761904745 1209 12 -1.2910052910052912 1209 13 -1.636243386243379 1209 14 -0.18650793650792963 1209 15 -1.6785714285714306 1209 16 -7.921957671957674 1209 17 -10.193121693121693 1209 18 -1.727513227513228 1209 19 -1.224867724867721 1209 20 -2.9576719576719555 1209 21 -8.132275132275137 1209 22 -9.990740740740737 1209 23 -2.2817460317460316 1209 24 0.8452380952380949 1209 25 -1.4894179894179942 1209 26 -5.767195767195769 1209 27 -1.5185185185185262 1209 28 -0.716931216931215 1209 29 -3.8253968253968154 1209 30 0.4325396825396908 1209 31 -5.675925925925927 1209 32 -1.533068783068778 1209 33 1.8478835978836017 1209 34 0.6150793650793673 1209 35 0.4629629629629548 1209 36 -6.4113756613756685 1209 37 -7.423280423280428 1209 38 -0.45899470899471595 1209 39 -9.064814814814806 1209 40 -5.914021164021161 1209 41 -5.189153439153447 1209 42 -8.044973544973548 1209 43 -7.120370370370377 1209 44 -0.6891534391534435 1209 45 -0.19973544973544222 1209 46 1.7566137566137527 1209 47 -4.837301587301585 1209 48 -6.273809523809522 1209 49 -0.5291005291005249 1209 50 -0.5423280423280445 1209 51 0.8320105820105823 1214 1 0.17460317460317754 1214 2 2.6521164021163983 1214 3 2.473544973544975 1214 4 -1.037037037037031 1214 5 -0.5502645502645578 1214 6 2.3888888888888857 1214 7 1.974867724867721 1214 8 2.673280423280424 1214 9 2.386243386243386 1214 10 2.6481481481481524 1214 11 1.8571428571428612 1214 12 2.708994708994709 1214 13 3.363756613756621 1214 14 3.146825396825406 1214 15 2.3214285714285694 1214 16 2.7447089947089935 1214 17 2.140211640211639 1214 18 3.272486772486772 1214 19 3.775132275132279 1214 20 3.0423280423280445 1214 21 2.5343915343915313 1214 22 3.3425925925925952 1214 23 2.051587301587304 1214 24 3.1785714285714306 1214 25 2.8439153439153415 1214 26 -0.10052910052910136 1214 27 2.481481481481474 1214 28 4.283068783068785 1214 29 3.1746031746031846 1214 30 3.7658730158730265 1214 31 2.9907407407407405 1214 32 3.466931216931222 1214 33 4.181216931216937 1214 34 3.948412698412703 1214 35 3.7962962962962905 1214 36 3.2552910052909994 1214 37 4.24338624338624 1214 38 3.541005291005284 1214 39 3.268518518518526 1214 40 3.419312169312171 1214 41 3.477513227513221 1214 42 2.62169312169312 1214 43 3.5462962962962905 1214 44 3.3108465608465565 1214 45 3.800264550264558 1214 46 4.089947089947088 1214 47 3.8293650793650826 1214 48 5.392857142857146 1214 49 3.470899470899475 1214 50 4.12433862433862 1214 51 3.8320105820105823 1215 1 0.17460317460317754 1215 2 -0.3478835978836017 1215 3 2.473544973544975 1215 4 -4.037037037037031 1215 5 -0.5502645502645578 1215 6 -1.6111111111111143 1215 7 1.974867724867721 1215 8 1.6732804232804241 1215 9 1.386243386243386 1215 10 -1.3518518518518476 1215 11 -1.1428571428571388 1215 12 -3.791005291005291 1215 13 -1.636243386243379 1215 14 -0.8531746031745939 1215 15 -10.17857142857143 1215 16 1.7447089947089935 1215 17 1.1402116402116391 1215 18 1.272486772486772 1215 19 1.775132275132279 1215 20 2.0423280423280445 1215 21 -1.4656084656084687 1215 22 1.3425925925925952 1215 23 1.051587301587304 1215 24 -0.8214285714285694 1215 25 1.8439153439153415 1215 26 1.8994708994708986 1215 27 1.4814814814814738 1215 28 -10.216931216931215 1215 29 -4.325396825396815 1215 30 -10.734126984126974 1215 31 -3.5092592592592595 1215 32 -11.033068783068778 1215 33 3.1812169312169374 1215 34 -13.551587301587297 1215 35 -4.2037037037037095 1215 36 -1.7447089947090006 1215 37 -3.25661375661376 1215 38 -1.958994708994716 1215 39 -0.7314814814814738 1215 40 -0.5806878306878289 1215 41 -0.5224867724867792 1215 42 1.62169312169312 1215 43 -0.4537037037037095 1215 44 2.3108465608465565 1215 45 -0.19973544973544222 1215 46 3.0899470899470884 1215 47 2.8293650793650826 1215 48 0.3928571428571459 1215 49 2.470899470899475 1215 50 -9.37566137566138 1215 51 2.8320105820105823 1212 1 -2.8253968253968225 1212 2 -12.847883597883602 1212 3 -10.026455026455025 1212 4 -1.037037037037031 1212 5 -3.550264550264558 1212 6 -1.6111111111111143 1212 7 -4.525132275132279 1212 8 -6.826719576719576 1212 9 -4.113756613756614 1212 10 0.6481481481481524 1212 11 0.8571428571428612 1212 12 0.7089947089947088 1212 13 -1.636243386243379 1212 14 -1.853174603174594 1212 15 -1.6785714285714306 1212 16 -16.255291005291006 1212 17 -1.8597883597883609 1212 18 -1.727513227513228 1212 19 -0.2248677248677211 1212 20 3.0423280423280445 1212 21 -1.4656084656084687 1212 22 2.3425925925925952 1212 23 1.051587301587304 1212 24 -9.32142857142857 1212 25 -6.6560846560846585 1212 26 -10.600529100529101 1212 27 1.4814814814814738 1212 28 -3.716931216931215 1212 29 -11.325396825396815 1212 30 1.7658730158730265 1212 31 -10.50925925925926 1212 32 -4.033068783068778 1212 33 -9.318783068783063 1212 34 1.948412698412703 1212 35 -1.2037037037037095 1212 36 -11.244708994709 1212 37 0.24338624338624015 1212 38 -3.458994708994716 1212 39 -3.731481481481474 1212 40 -15.580687830687829 1212 41 -6.022486772486779 1212 42 -1.37830687830688 1212 43 -9.95370370370371 1212 44 2.3108465608465565 1212 45 2.800264550264558 1212 46 3.0899470899470884 1212 47 -9.670634920634917 1212 48 -9.107142857142854 1212 49 -3.529100529100525 1212 50 -5.37566137566138 1212 51 -0.1679894179894177 1213 1 3.1746031746031775 1213 2 2.6521164021163983 1213 3 2.473544973544975 1213 4 1.962962962962969 1213 5 2.449735449735442 1213 6 1.3888888888888857 1213 7 0.9748677248677211 1213 8 1.6732804232804241 1213 9 1.386243386243386 1213 10 0.6481481481481524 1213 11 0.8571428571428612 1213 12 0.7089947089947088 1213 13 3.363756613756621 1213 14 3.146825396825406 1213 15 3.3214285714285694 1213 16 3.7447089947089935 1213 17 3.140211640211639 1213 18 3.272486772486772 1213 19 3.775132275132279 1213 20 4.0423280423280445 1213 21 1.5343915343915313 1213 22 1.3425925925925952 1213 23 1.051587301587304 1213 24 2.1785714285714306 1213 25 1.8439153439153415 1213 26 1.8994708994708986 1213 27 3.481481481481474 1213 28 4.283068783068785 1213 29 3.1746031746031846 1213 30 1.7658730158730265 1213 31 1.9907407407407405 1213 32 3.466931216931222 1213 33 3.1812169312169374 1213 34 1.948412698412703 1213 35 3.7962962962962905 1213 36 3.2552910052909994 1213 37 2.24338624338624 1213 38 4.541005291005284 1213 39 2.268518518518526 1213 40 2.419312169312171 1213 41 -3.522486772486779 1213 42 1.62169312169312 1213 43 2.5462962962962905 1213 44 2.3108465608465565 1213 45 2.800264550264558 1213 46 3.0899470899470884 1213 47 2.8293650793650826 1213 48 5.392857142857146 1213 49 2.470899470899475 1213 50 3.1243386243386198 1213 51 4.832010582010582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 2.0 24 7.0 25 7.0 26 9.0 27 16.0 28 18.0 29 26.0 30 37.0 31 45.0 32 66.0 33 53.0 34 72.0 35 87.0 36 96.0 37 172.0 38 270.0 39 325.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.35348125478194 20.964039785768936 13.618974751338945 31.063504208110178 2 11.153552330022919 23.300229182582125 44.919786096256686 20.626432391138273 3 14.667685255920551 25.362872421695954 39.419404125286476 20.55003819709702 4 12.070282658517954 22.15431627196333 39.877769289533994 25.89763177998472 5 12.757830404889228 35.90527119938884 38.961038961038966 12.375859434682965 6 24.293353705118413 44.690603514132924 19.098548510313215 11.917494270435448 7 23.45301757066463 32.467532467532465 25.821237585943468 18.258212375859433 8 21.008403361344538 40.33613445378151 21.84873949579832 16.80672268907563 9 22.61268143621085 19.556913674560732 19.63330786860199 38.19709702062643 10 14.667685255920551 33.76623376623377 29.717341482047367 21.84873949579832 11 29.02979373567609 26.355996944232242 28.189457601222305 16.424751718869366 12 17.79984721161192 26.661573720397254 33.07868601986249 22.459893048128343 13 21.695951107715814 22.918258212375857 32.69671504965623 22.689075630252102 14 19.556913674560732 20.397249809014514 27.57830404889228 32.467532467532465 15 20.24446142093201 28.57142857142857 24.980901451489686 26.203208556149733 16 21.84873949579832 30.786860198624904 26.508785332314744 20.855614973262032 17 20.168067226890756 31.779984721161192 30.17570664629488 17.87624140565317 18 26.355996944232242 27.57830404889228 27.960275019098546 18.105423987776927 19 22.307104660045837 25.97402597402597 27.42551566080978 24.293353705118413 20 20.55003819709702 26.203208556149733 27.501909854851032 25.744843391902215 21 20.01527883880825 26.203208556149733 26.203208556149733 27.57830404889228 22 18.792971734148207 33.30786860198625 26.967150496562258 20.932009167303285 23 19.63330786860199 29.793735676088616 27.73109243697479 22.841864018334608 24 25.057295645530942 29.488158899923604 25.821237585943468 19.63330786860199 25 20.855614973262032 33.3842627960275 27.883880825057293 17.87624140565317 26 25.439266615737203 27.807486631016044 25.744843391902215 21.008403361344538 27 19.022154316271962 33.460656990068756 27.807486631016044 19.709702062643238 28 20.473644003055767 27.883880825057293 27.883880825057293 23.758594346829643 29 19.709702062643238 27.807486631016044 28.49503437738732 23.9877769289534 30 18.105423987776927 32.23834988540871 29.02979373567609 20.626432391138273 31 24.675324675324674 29.946524064171122 25.744843391902215 19.63330786860199 32 19.404125286478227 32.69671504965623 28.113063407181055 19.786096256684495 33 21.92513368983957 25.744843391902215 32.773109243697476 19.556913674560732 34 22.15431627196333 26.814362108479756 25.515660809778456 25.515660809778456 35 18.029029793735678 26.814362108479756 26.508785332314744 28.647822765469826 36 20.32085561497326 26.43239113827349 32.391138273491215 20.855614973262032 37 18.41100076394194 31.856378915202445 28.953399541634834 20.77922077922078 38 16.730328495034378 25.821237585943468 34.988540870893814 22.459893048128343 39 18.563789152024444 22.841864018334608 32.23834988540871 26.355996944232242 40 18.41100076394194 23.52941176470588 34.30099312452254 23.758594346829643 41 19.25133689839572 22.689075630252102 30.40488922841864 27.65469824293354 42 22.15431627196333 22.38349885408709 31.779984721161192 23.682200152788386 43 17.3414820473644 22.459893048128343 35.9816653934301 24.216959511077157 44 17.11229946524064 30.02291825821238 31.016042780748666 21.84873949579832 45 17.188693659281896 22.536287242169596 35.9816653934301 24.293353705118413 46 22.689075630252102 24.44614209320092 30.786860198624904 22.07792207792208 47 15.508021390374333 24.369747899159663 31.62719633307869 28.49503437738732 48 16.424751718869366 24.216959511077157 29.488158899923604 29.87012987012987 49 20.55003819709702 23.911382734912145 30.40488922841864 25.13368983957219 50 16.195569136745608 23.834988540870896 38.12070282658518 21.84873949579832 51 14.514896867838045 24.980901451489686 31.321619556913678 29.182582123758593 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.5 16 1.0 17 0.5 18 0.0 19 0.0 20 0.0 21 0.5 22 1.0 23 1.0 24 1.0 25 3.0 26 8.0 27 11.0 28 20.0 29 29.0 30 36.0 31 43.0 32 57.0 33 71.0 34 85.0 35 99.0 36 121.5 37 144.0 38 147.0 39 150.0 40 139.0 41 128.0 42 127.5 43 127.0 44 107.0 45 87.0 46 84.0 47 81.0 48 79.5 49 78.0 50 94.5 51 111.0 52 80.5 53 50.0 54 32.0 55 14.0 56 16.5 57 19.0 58 15.0 59 11.0 60 12.0 61 13.0 62 8.5 63 4.0 64 3.0 65 2.0 66 5.5 67 9.0 68 8.0 69 7.0 70 6.0 71 5.0 72 5.0 73 5.0 74 3.0 75 0.5 76 0.0 77 0.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15278838808250572 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1309.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.34682964094728 #Duplication Level Percentage of deduplicated Percentage of total 1 99.19028340080972 93.58288770053476 2 0.4048582995951417 0.7639419404125287 3 0.08097165991902834 0.2291825821237586 4 0.0 0.0 5 0.08097165991902834 0.38197097020626436 6 0.0 0.0 7 0.0 0.0 8 0.16194331983805668 1.2223071046600458 9 0.0 0.0 >10 0.08097165991902834 3.819709702062643 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 50 3.819709702062643 No Hit TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT 8 0.6111535523300229 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 8 0.6111535523300229 RNA PCR Primer, Index 27 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC 5 0.38197097020626436 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 3 0.2291825821237586 TruSeq Adapter, Index 12 (95% over 21bp) GTACTTGTTCACTATCGGTCTTTCAGGAGTATTTAGCCTGTCTCTTATACA 2 0.15278838808250572 No Hit CTTCCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGAC 2 0.15278838808250572 No Hit GGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGGCTTTACCCGATA 2 0.15278838808250572 No Hit GACAGGTATCGTAAATTTATTTACGAGGCAAACCGGGAGAAGTGAAACATC 2 0.15278838808250572 No Hit CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTCTGTCTCTT 2 0.15278838808250572 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.07639419404125286 24 0.0 0.0 0.07639419404125286 25 0.0 0.0 0.07639419404125286 26 0.0 0.0 0.07639419404125286 27 0.0 0.0 0.07639419404125286 28 0.0 0.0 0.07639419404125286 29 0.0 0.0 0.45836516424751717 30 0.0 0.0 0.8403361344537815 31 0.0 0.0 1.8334606569900687 32 0.0 0.0 2.4446142093200915 33 0.0 0.0 3.361344537815126 34 0.0 0.0 3.972498090145149 35 0.0 0.0 5.271199388846448 36 0.0 0.0 6.187929717341482 37 0.0 0.0 7.639419404125286 38 0.0 0.0 9.931245225362872 39 0.0 0.0 11.841100076394195 >>END_MODULE >>Kmer Content pass >>END_MODULE