FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_39.3410000000a1bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_39.3410000000a1bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047226
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC105180.513768387075975No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT24490.11962528807273844No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCTGTCTCTT24270.11855066319009236No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTCTGTCTCTT21840.10668094289541068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13600.040.6979145
ATGCCGT15650.035.07933844
TATGCCG16250.033.784143
CCCACGA16400.033.7486619
CCACGAG16500.033.54412520
CGTATGC16800.032.67727741
GCCCACG17000.032.55753318
ACGAGAC17450.031.97581122
CACGAGA17400.031.80908421
TCGTATG17450.031.46007240
GTATGCC17650.031.10358442
CGAGACT17900.031.04625723
CCTATCT17850.030.6290434
CTCGTAT18200.030.04001839
TCTCGTA18500.029.55288538
TATCTCG18550.029.47322736
GAGACTA18950.029.44474424
CGAGCCC18850.029.36223415
CTAGATC18900.029.28455528
AGCCCAC19100.029.09570517
Produced by FastQC (version 0.11.2)