FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_38.3410000000a1a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_38.3410000000a1a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698083
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC21890.12891007094470647TruSeq Adapter, Index 12 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG4450.034.8865918
TATGCCG4400.034.77168343
TGCCGTC4550.034.11985445
AGCCCAC4900.032.1418917
ATGCCGT4800.031.8740444
CGTATGC4950.031.36269441
CCGAGCC5000.031.04906714
CCCACGA5150.030.14472619
CGAGCCC5150.030.14472615
GTATGCC5100.029.99909842
CTCCGAG5500.029.0445812
GAGCCCA5450.028.89821216
TCCGAGC5550.027.97213213
CGAGACT5500.027.81734523
CCACGAG5800.027.15435220
TCGTATG6200.025.4024640
ACGAGAC6200.025.4024622
CACGAGA6450.024.76669521
CTCGTAT6400.023.9055339
TCTCGTA6600.023.52201738
Produced by FastQC (version 0.11.2)