FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_37.3410000000a187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_37.3410000000a187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191266
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG2050.028.5358419
GCGCGCT3600.027.49928311
GGTATCA5450.026.4524171
CGTCGGG2300.026.41235214
GTCGCGC2300.025.4341168
CGCGCGC2300.025.43411610
TATGCCG851.894241E-723.82290643
ATACCCG2750.023.7266525
TACCCGG2600.023.3647776
ACCTATA2900.023.302641
GGTCGCG2550.022.9405777
CCTAGAC1003.6456186E-822.4994133
TGCGCGC1850.021.89132110
GCGCTAA1850.021.89132113
GCCAGGC2800.021.69586227
CGCGCTA1900.021.31523312
ACTGCGC2050.020.8531138
GCCCACG1009.0577487E-720.24947218
GCGCCAT2900.020.17188621
GGGGCTA2900.020.17188636
Produced by FastQC (version 0.11.2)