FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_3.34100000009f49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_3.34100000009f49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231499
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15480.6686853938893905No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA13140.5676050436502965No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11280.4872591242294783No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10440.45097387029749586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.44060665488835804No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8330.35982876815882575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.344709912353833No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7930.3425500758102627No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC6300.27213940448986823No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5710.2466533332757377No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4600.1987049620084752No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4220.18229020427734027No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.16328364269392093No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.14082134264078894No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2980.1287262579967948No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2800.12095084643994143No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2560.11058363103080358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGA700.038.5703221
GAGACTA700.038.5703224
TCGTATG700.038.5703240
GACTAGA659.094947E-1238.0758326
GTATGCC801.8189894E-1233.74902742
AGATCCT754.7293724E-1132.9990530
TATCTCG754.7293724E-1132.9990536
CTCGTAT855.456968E-1231.7637939
ATCCTAT801.0004442E-1030.9366132
TAGATCC801.0004442E-1030.9366129
CGAGACT1001.8189894E-1229.24915923
ACGAGAC1001.8189894E-1229.24915922
CGTATGC952.3646862E-1128.42023541
AGACTAG903.9108272E-1027.49920725
TATGCCG1004.5474735E-1126.99922443
GATCCTA957.348717E-1026.05188231
CCCACGA1058.367351E-1125.71354519
TCTCGTA1058.367351E-1125.71354538
ATGCCGT1058.367351E-1125.71354544
AAAGTGT450.00122380224.9992796
Produced by FastQC (version 0.11.2)