FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_26.3410000000a0d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_26.3410000000a0d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490139
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC21410.14367787166163695TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2800.027.3204318
TATGCCG2800.026.51777643
ATGCCGT2950.025.16941644
CGTATGC3050.025.08105341
CCCACGA3250.024.92216719
GTATGCC3350.022.83498842
TACCCGG2550.021.1756976
TCGCGCG1850.020.674929
CGTCGGG2550.020.29337714
GCGCGCT3350.020.14851811
CGCGCGC1800.019.9992710
TCGTATG3850.019.86940440
CGGGGCA3100.019.59605817
GCGCGAC1051.4466623E-619.2850111
CTGCGCG1757.2759576E-1219.285019
GGGTCGC951.3166738E-518.94667638
GGGCCGG1555.347829E-1018.87027741
GTATCAA20750.018.7873481
CGGCACG851.2050827E-418.52873421
TGCGCGC1709.276846E-1118.52873410
Produced by FastQC (version 0.11.2)