FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_25.3410000000a0be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_25.3410000000a0be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627828
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA16410.26137731990290336No Hit
TGGCTAGATCGATCGGTTTCGGGTCAAATAGGAAGAACTAGAAGATTCCAC12260.19527641328516726No Hit
ATCTAGCCATGAGCAGGTTGAAGAGAGCTCTAACAGGCCTTGGAGGACCGA12120.19304650318240027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11830.18842740368381147No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA11680.1860382142879897No Hit
CTCTAACGGCGTACCTTTTGCATGATGGGTCAGCGAGGAAATGGGAAGAGC11350.18078199761718178No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGATCGGGAAACCGTATCCAA10840.1726587536713877No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10500.167243257707525No Hit
CTTTAGGAATCTTCAGTCAAGGTACGAAGTAACTCGACTGAAAGAAGAGGG10170.16198704103671707No Hit
TTCCTAAAGGGCGCTGTGAACTGTAATTGTGAAAAGGTTGGAAGATCTGGC9530.15179316628121078No Hit
TTCGTGTACCCATCGGACGGCAGCCCTTTCGGGGGTTCCTTAGGGACCGAT9310.1482890218340055No Hit
CTCCAGGTCTTGTCACCAAAAACCTTCCCCGATTGACAGAACGTTCCGCTA9210.14669622890345763No Hit
CTTTGGAACGAAGCTTCGTCTTTCTTTTTCTTAAAATCCCAAATCCGCTCT9040.14398848092152627No Hit
CCGTTAGAGTGAGTGCGCTTGACTACTCCTTCGACTGATTGTTCGCATCGG8990.14319208445625237No Hit
GGCTTAGAGGGTGGTCCCCCTTTCTCGCGTAAAAGCGATCAGAATTCGAAC8870.14128073293959492No Hit
ACTATAAACAGACTCGCGACTATGGCAGGGCGGTACGCTCTGCTCTCTCGC8690.13841370566460875No Hit
CTCCAAGAGAGAGGCATGGTTTTGTAGTCAAAGCAACTTCGTCACTTTCGT8370.13331676828685562No Hit
CTCTTCAACCTGCTCATGGCTAGATCGATCGGTTTCGGGTCAAATAGGAAG7800.12423784858273286No Hit
CTCTAAGCCTAAGTATTCCTCAATGACCGATAGCGTACAAGTACCGTGAGG7610.12121154201469192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7490.1193001904980345No Hit
GACCTGGAGATATCAGAAGTGAGAATGCTGACATGAGTAACGATAAATCCT7420.118185235446651No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7220.11499964958555527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.11229190160362391No Hit
GAGCTAGACCAGTGAGCTATTACGCTTTCTTCAAAGGATGGCTGCTTCCAA6920.1102212707939117No Hit
GTATTCGGAGTTTCCCTGGGGTTGGTAAGGCGAAATGGGGCCACCCTAGCC6920.1102212707939117No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG6910.11006199150085692No Hit
GCGTAGATTGACTGAACGCAGAAAGCCTTCCACTGGCAGGCGATCGTGTTT6890.10974343291474736No Hit
GAGTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACG6850.1091063157425282No Hit
GTATCAACGCAGAGTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAG6570.1046464955369942No Hit
GTACACGAAAGTGACGAAGTTGCTTTGACTACAAAACCATGCCTCTCTCTT6530.10400937836477506No Hit
GTCTAGCTCCATGGCACCGAAAATGTATCAGGGCTCAAGTGATTCACCGAA6530.10400937836477506No Hit
GTTCCAAAGTGTTCACTTCTTTTTCGCCAGGTTTCATTCGATTTATTGTGG6480.10321298189950115No Hit
CTACTAACGGGGTCTCGGTTGATTTCCCTTCCTTTAGCTACTTAGATGTTT6450.10273514402033679No Hit
GTCCAAAGAGCGCAGACTAGCCACGGAGCTTGGATACGGTTTCCCGATCGG6380.10162018896895328No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6310.10050523391756977No Hit
CTACTGAAGGTCGCCAAACTACGACGAGACTTTCGCCTTTTGAAGCGCCAG6310.10050523391756977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC458.693369E-734.9990735
TCGTATG708.1308826E-1032.14200640
GTAACGC352.8193675E-432.14200618
ATGCTAG453.4606222E-530.0350841
CCGTTTA702.8825525E-828.92780527
CGATAGG406.163958E-428.12425439
CGTATGC856.4719643E-926.46988741
GCCCACG901.1892553E-824.99933618
CGAGACG901.1892553E-824.99933623
GAATTGC901.1892553E-824.99933629
GACGAAT901.1892553E-824.99933626
GCGCTAT450.001226124624.9993368
ATGTAAT551.3564315E-424.574161
CGCGATG551.3674835E-424.54480221
ACCTATT551.3674835E-424.54480233
TCCGAGC952.1122105E-823.68358213
TATGCCG952.1122105E-823.68358243
CCCACGA952.1122105E-823.68358219
TACCTAT702.701023E-522.49940532
ATGGGAG602.4662216E-422.49940325