FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_23.3410000000a095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_23.3410000000a095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC402.6881720430107525No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG140.9408602150537635No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC110.739247311827957RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT100.6720430107526881No Hit
CCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCT70.47043010752688175No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC70.47043010752688175No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC60.4032258064516129No Hit
GACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGA50.33602150537634407No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC50.33602150537634407TruSeq Adapter, Index 12 (95% over 21bp)
CGTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGA50.33602150537634407No Hit
ACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCT50.33602150537634407No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTC40.2688172043010753No Hit
GTATCTATCCATACACCCTAAAAAAGTTCGCAATAATAAAACCTGTCTCTT40.2688172043010753No Hit
AAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAATATTCTTGCATGGAAT40.2688172043010753No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA40.2688172043010753No Hit
TCCTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGA30.20161290322580644No Hit
GTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTC30.20161290322580644No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT30.20161290322580644No Hit
GATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTCTTATTTT30.20161290322580644No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTC30.20161290322580644No Hit
AACTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGTTCAAATC20.13440860215053765No Hit
AAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGG20.13440860215053765No Hit
CTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGAGG20.13440860215053765No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG20.13440860215053765No Hit
GTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAA20.13440860215053765No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTAC20.13440860215053765No Hit
CGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACT20.13440860215053765No Hit
CTTCTAACTTTTCTTAGAAAAATCAAATTCACTTTAAGCTCAATTCATAAA20.13440860215053765No Hit
ATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTTC20.13440860215053765TruSeq Adapter, Index 27 (95% over 22bp)
TAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTA20.13440860215053765No Hit
ATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCTAATTT20.13440860215053765No Hit
GTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGC20.13440860215053765No Hit
ATTCATAAAAGCACCTATTACTCCACTCATACCCGTACTCTGCGTTGATAC20.13440860215053765No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA20.13440860215053765No Hit
CTAATTATCCTAAGATAAAATCCAATCTGGCTCACGCCGATTTGAACTCAG20.13440860215053765No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCT20.13440860215053765No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTGTC20.13440860215053765No Hit
GTGCTAAGGTAGCGAAGTCATTTGTTTCTTTATTGGGAACTTGTATGAGTG20.13440860215053765No Hit
GGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGA20.13440860215053765No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAACTGTCTCTTAT20.13440860215053765No Hit
CAGCTCATGTTCAATTTTAATAGTCGAACAGACTATTAGTTTGAAAAGCTA20.13440860215053765No Hit
CAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTA20.13440860215053765No Hit
GGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAA20.13440860215053765No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA20.13440860215053765No Hit
CTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCCTGTCTCTTATACA20.13440860215053765No Hit
GACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATAT20.13440860215053765No Hit
ACCTAGATCTTATTCCATTATTCCATGCAAGAATATCTGTCTCTTATACAC20.13440860215053765No Hit
TTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCTTAT20.13440860215053765No Hit
AGTCAATAAAAGTACTCAGACTGATCGCTGGTTGGCATCGTTTATGGTTAG20.13440860215053765No Hit
GCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCTTATACACA20.13440860215053765No Hit
ATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTG20.13440860215053765No Hit
TAATTACAATGCCCAGTCCCTATTACGAAGAATTTTCATATGGTTACCCGC20.13440860215053765No Hit
ATTAAAATTGAACATGAGCTGAGTTCAAATCGGCGTGAGCCAGATTGGATT20.13440860215053765No Hit
TAGTTGGGGGTAGCTTTTCAAACTAATAGTCTGTTCGACTATTAAAATTGA20.13440860215053765No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGGGACCTG20.13440860215053765No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)