FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_21.3410000000a07b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_21.3410000000a07b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC221.8739352640545146No Hit
GACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGA60.5110732538330494No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG60.5110732538330494No Hit
ATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTG50.42589437819420783No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC30.2555366269165247No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG30.2555366269165247No Hit
GTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAA30.2555366269165247No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC30.2555366269165247RNA PCR Primer, Index 27 (95% over 21bp)
ATCGGGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGC30.2555366269165247No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC30.2555366269165247TruSeq Adapter, Index 12 (95% over 21bp)
CTTTTGAAGTTCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTT20.17035775127768313No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATCTGTCTCTTATAC20.17035775127768313No Hit
GAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAATATTCTTGCATG20.17035775127768313No Hit
GTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTA20.17035775127768313No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTGTCTCTTATAC20.17035775127768313No Hit
ACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTTCTG20.17035775127768313RNA PCR Primer, Index 27 (96% over 26bp)
CTCTAACTTTCGTTCTTGATTAATGAAAACATTCCTGTCTCTTATACACAT20.17035775127768313No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTGTC20.17035775127768313No Hit
ACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTC20.17035775127768313No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC20.17035775127768313No Hit
GGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAA20.17035775127768313No Hit
GCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGCTGTCTCTTATA20.17035775127768313No Hit
ATCGGGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCCTGTCTCTTAT20.17035775127768313No Hit
TAAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTG20.17035775127768313No Hit
GACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATAT20.17035775127768313No Hit
ACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCT20.17035775127768313No Hit
AAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAATATTCTTGCATGGAAT20.17035775127768313No Hit
TAATTACAATGCCCAGTCCCTATTACGAAGAATTTTCATATGGTTACCCGC20.17035775127768313No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGCTGTCTC20.17035775127768313No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)