FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_20.3410000000a06e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_20.3410000000a06e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC322.6143790849673203No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT80.6535947712418301No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG80.6535947712418301No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA50.40849673202614384No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG30.24509803921568626No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAG30.24509803921568626No Hit
GTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTC30.24509803921568626No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC30.24509803921568626RNA PCR Primer, Index 27 (95% over 21bp)
GCTCATGTTCAATTTTAATAGTCGAACAGACTATTAGTTTGAAAAGCTACC30.24509803921568626No Hit
GTGCTAAGGTAGCGAAGTCATTTGTTTCTTTATTGGGAACTTGTATGAGTG30.24509803921568626No Hit
CAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATCTG20.16339869281045752No Hit
GACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGA20.16339869281045752No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC20.16339869281045752No Hit
GTTCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTTGATTTTAG20.16339869281045752No Hit
CTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGAGG20.16339869281045752No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG20.16339869281045752No Hit
ATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCTAATTTTTTCA20.16339869281045752No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTC20.16339869281045752No Hit
GTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACT20.16339869281045752No Hit
GATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTCTTATTTT20.16339869281045752No Hit
CATTTGAATATTTGCTACTACCACCAAGATCTGCACCTGTCTCTTATACAC20.16339869281045752No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTC20.16339869281045752No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCT20.16339869281045752No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.16339869281045752RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20.16339869281045752TruSeq Adapter, Index 12 (95% over 21bp)
ATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAA20.16339869281045752No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCTG20.16339869281045752No Hit
GTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCTGTC20.16339869281045752No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCCTGTCT20.16339869281045752No Hit
CTTTTCAAACTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGT20.16339869281045752No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA20.16339869281045752No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGCT20.16339869281045752No Hit
GCCCCCGTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGCTGTCTCTT20.16339869281045752No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)