FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_2.34100000009f3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_2.34100000009f3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418852
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.2915110826735935No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9280.22155797274454936No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8480.20245814750795033No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC8410.20078691279974786TruSeq Adapter, Index 12 (95% over 21bp)
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8260.19720569556788559No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG8240.19672819993697058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.1814483397476913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.15542482786282505No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6460.15423108878553762No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5950.14205495019720568No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT5770.1377574895189709No Hit
CTCTAACGGCGTACCTTTTGCATGATGGGTCAGCGAGGAAATGGGAAGAGC5760.13751874170351341No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC5670.13537001136439603No Hit
TGGCTAGATCGATCGGTTTCGGGTCAAATAGGAAGAACTAGAAGATTCCAC5170.12343262059152159No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5020.11985140335965927No Hit
ATCTAGCCATGAGCAGGTTGAAGAGAGCTCTAACAGGCCTTGGAGGACCGA4980.1188964120978293No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC4830.11531519486596697No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATG4740.11316646452684959No Hit
CCGTTAGAGTGAGTGCGCTTGACTACTCCTTCGACTGATTGTTCGCATCGG4650.11101773418773217No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT4650.11101773418773217No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAA4620.11030149074135973No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA4460.1064815256940399No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA4260.10170656938489012No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA4250.10146782156943263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAATC501.0786607E-940.49849732
GCCCCGC301.1389326E-437.49860812
CCGCTGT301.1389326E-437.49860815
ACCTGCA458.6849104E-734.998739
GTATGCC1300.032.88339242
CGATAGG352.817952E-432.14166313
CGTATGC1350.031.6654941
CTCGTAT1350.031.6654939
TCGTATG1400.030.53457840
GACCATG300.005144502529.9988867
CATGTGG300.005144502529.99888614
GCAATCT1450.029.48166534
TTGCAAT1450.029.48166532
ACGACTG554.157837E-628.63536
ATGCCGT1500.028.49894144
CAATCTC1500.028.49894135
CTCCGAC406.160886E-428.12395722
CACGAGA1600.028.12395721
TGTAACG406.160886E-428.1239572
TACTTGC1550.027.5796229
Produced by FastQC (version 0.11.2)