FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_19.3410000000a052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_19.3410000000a052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC393.6077705827937097No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC131.2025901942645698RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT80.7400555041628123No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG80.7400555041628123No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC50.46253469010175763No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTC50.46253469010175763No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA50.46253469010175763No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAG40.37002775208140615No Hit
CCTAAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGT40.37002775208140615No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATCTGTCT40.37002775208140615No Hit
GCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACC30.27752081406105455No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTC30.27752081406105455No Hit
CCCTCTAATTATCCTAAGATAAAATCCAATCTGGCTCACGCCGATTTGAAC30.27752081406105455No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG30.27752081406105455No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGCTGTCTC30.27752081406105455No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC20.18501387604070307No Hit
AAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGG20.18501387604070307No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG20.18501387604070307No Hit
CCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGA20.18501387604070307No Hit
GCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAAC20.18501387604070307No Hit
GATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAA20.18501387604070307No Hit
GTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCTGTCTCTTATAC20.18501387604070307No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCCTGTCTCT20.18501387604070307No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCT20.18501387604070307No Hit
GTTTTATTATTGCGAACTTTTTTAGGGTGTATGGATAGATACCTGTCTCTT20.18501387604070307No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTCTTATAC20.18501387604070307No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20.18501387604070307TruSeq Adapter, Index 12 (95% over 21bp)
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGCTGTCTCTTATA20.18501387604070307No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGA20.18501387604070307No Hit
ATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTTC20.18501387604070307RNA PCR Primer, Index 27 (95% over 24bp)
ATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTG20.18501387604070307No Hit
TCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAATA20.18501387604070307No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA20.18501387604070307No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGAT20.18501387604070307No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)