FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_17.3410000000a038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_17.3410000000a038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC413.399668325041459No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT70.5804311774461027No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC60.4975124378109453No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG50.41459369817578773No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAG40.33167495854063017No Hit
GTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAA40.33167495854063017No Hit
GCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAAC40.33167495854063017No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTC40.33167495854063017No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC40.33167495854063017RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC40.33167495854063017TruSeq Adapter, Index 12 (95% over 21bp)
GATTTGAACTCAGCTCATGTTCAATTTTAATAGTCGAACAGACTATTAGTT40.33167495854063017No Hit
GACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGA30.24875621890547264No Hit
CCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCT30.24875621890547264No Hit
AATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAA30.24875621890547264No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTCTTATACAC30.24875621890547264No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTC30.24875621890547264No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA30.24875621890547264No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGAT30.24875621890547264No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGCTGTCTC30.24875621890547264No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC20.16583747927031509No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACCTGTCTCTTA20.16583747927031509No Hit
AAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGG20.16583747927031509No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG20.16583747927031509No Hit
AAGCAGGCATTTCGCCTAAATATTCTTGCATGGAATAATGGAATAAGATCT20.16583747927031509No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGGGCTGTC20.16583747927031509No Hit
CATTAATCAAGAACGAAAGTTAGAGGTTCGAAGGCGATCAGATACCTGTCT20.16583747927031509No Hit
ATCGGGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGC20.16583747927031509No Hit
GTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAA20.16583747927031509No Hit
TTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTT20.16583747927031509No Hit
TCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTTGATTTTAGTT20.16583747927031509No Hit
CTTATACACACTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.16583747927031509RNA PCR Primer, Index 27 (95% over 21bp)
GACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATAT20.16583747927031509No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC150.0099145644.99999617
GTATGCC150.0099145644.99999642
ATCTCGT150.0099145644.99999637
ATCTCCG150.0099145644.99999610
CACATCT150.0099145644.9999967
GCCCACG150.0099145644.99999618
TATACAC150.0099145644.9999963
TGCCGTC150.0099145644.99999645
CCACGAG150.0099145644.99999620
TATGCCG150.0099145644.99999643
CATCTCC150.0099145644.9999969
GAGCCCA150.0099145644.99999616
CACGAGA150.0099145644.99999621
CCCACGA150.0099145644.99999619
ACATCTC150.0099145644.9999968
TCTCGTA150.0099145644.99999638
ACGAGAC150.0099145644.99999622
ATGCCGT150.0099145644.99999644
CGTATGC150.0099145644.99999641
CGAGCCC150.0099145644.99999615
Produced by FastQC (version 0.11.2)