FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_16.3410000000a02b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_16.3410000000a02b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC292.4451939291736933No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG70.5902192242833052No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA70.5902192242833052No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT60.5059021922428331No Hit
GACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGA50.42158516020236086No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTC50.42158516020236086No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA50.42158516020236086No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC50.42158516020236086RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC50.42158516020236086No Hit
CGTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGA40.33726812816188867No Hit
AAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAATATTCTTGCATGGAAT40.33726812816188867No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC30.25295109612141653No Hit
CTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGAGG30.25295109612141653No Hit
GTTTTATTATTGCGAACTTTTTTAGGGTGTATGGATAGATACCTGTCTCTT30.25295109612141653No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC30.25295109612141653TruSeq Adapter, Index 12 (95% over 21bp)
AACTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGTTCAAATC20.16863406408094433No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG20.16863406408094433No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGCTGTCTCTTATACAC20.16863406408094433No Hit
GTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAA20.16863406408094433No Hit
GCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAAC20.16863406408094433No Hit
GCACTTGCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTACTC20.16863406408094433No Hit
CTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGCTGTCTCTTATA20.16863406408094433No Hit
GTGTAACCTAAATTAGGAGGTAAACTTCTCCTAAGACTAAATGATTTGGCA20.16863406408094433No Hit
CCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCT20.16863406408094433No Hit
GATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTCTTATTTT20.16863406408094433No Hit
GTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGC20.16863406408094433No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCTGTCTCTT20.16863406408094433No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTCTGTC20.16863406408094433No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.16863406408094433RNA PCR Primer, Index 27 (95% over 21bp)
GATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTCTTATTTTAGGG20.16863406408094433No Hit
GTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAA20.16863406408094433No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACA20.16863406408094433No Hit
CAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTA20.16863406408094433No Hit
TGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCT20.16863406408094433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA20.16863406408094433No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGCT20.16863406408094433No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)