FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_15.3410000000a01e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_15.3410000000a01e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371112
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG360781.5215645654865735No Hit
GTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAA198190.835852545134941No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC189790.8004261291748345No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA166170.7008104214393921No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA137860.5814149647928905No Hit
GACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGA137420.5795592953854563No Hit
CCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCT134290.5663587380098452No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTC127410.5373428163663294No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGAT124930.5268835887971551No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAG120340.5075255829332398No Hit
ACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCT112780.47564180856914395No Hit
AAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAATATTCTTGCATGGAAT99580.4199717263461194No Hit
GGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAA94410.3981676108087682No Hit
GATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTCTTATTTT70240.29623231631403324No Hit
CGTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGA69990.29517795869617297No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTC69940.2949670871726009No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGGGACAGA69160.2916774914048767No Hit
ATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTG69140.2915931427954479No Hit
GCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAAC67310.2838752450327104No Hit
CTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGAGG65470.27611517296525845No Hit
GATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAA63480.2677224863270904No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG62970.2655715967866554No Hit
GTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGC62520.2636737530745068No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTC62370.26304113850379063No Hit
CTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGTTCAAATCGG61890.26101677187749883No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGCTGTCTC57810.24380965555401854No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTCTTATAC57320.24174311462301235No Hit
GTAATAAACTTCCCCTAAAATAAGAACTTCAAAAGAAAATAATCCTGTTAC57110.24085745422400967No Hit
GATTTGAACTCAGCTCATGTTCAATTTTAATAGTCGAACAGACTATTAGTT57010.24043571117686552No Hit
TTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTT56210.23706176679971253No Hit
GTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAA55200.23280216202355686No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCTTATACACATCTCC54800.2311151898349804No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC54630.23039822665483536No Hit
GTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTC52920.22318642054867086No Hit
GTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTA52420.22107770531295023No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATCTGTCTCTTATAC50960.21492025682464602No Hit
GTACTAAACACCCTCTAATTATCCTAAGATAAAATCCAATCTGGCTCACGC50610.21344415615964155No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTGTC49730.20973281734477325No Hit
CTAATTATCCTAAGATAAAATCCAATCTGGCTCACGCCGATTTGAACTCAG44350.18704304140841932No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCTGTCTCTTATACACATCTCC43390.18299430815583573No Hit
AACTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGTTCAAATC43060.18160255610026013No Hit
CAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTA42010.17717425410524681No Hit
CCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGAT40340.17013114521793996No Hit
GTATCTATCCATACACCCTAAAAAAGTTCGCAATAATAAAACCTGTCTCTT39830.1679802556775049No Hit
CCTAAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGT39400.16616676057478516No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGCT37680.15891278016390623No Hit
GTTCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTTGATTTTAG37570.15844886281204767No Hit
TTTTTAGGGTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTTCTTT37200.1568884135376144No Hit
GTTTTATTATTGCGAACTTTTTTAGGGTGTATGGATAGATACCTGTCTCTT36720.1548640469113226No Hit
ATCGGGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGC36290.15305055180860289No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAAGGCGATCAGA35710.15060444213516697No Hit
CTTTTGAAGTTCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTT33190.139976517347135No Hit
GTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCTGTC32900.13875346251041706No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCCTGTCT32440.13681344449355407No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACA32380.1365603986652676No Hit
TTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAAAC32150.13559038965683612No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAACTGTCTCTTAT32100.13537951813326407No Hit
ATTTTAATAGTCGAACAGACTATTAGTTTGAAAAGCTACCCCCAACTAAAA32020.13504212369554877No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATCTGTCT31990.13491560078140552No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTCTTATACAC31000.13074034461467868No Hit
GACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATAT30990.13069817030996428No Hit
GTTTTATTATTGCGAACTTTTTTAGGGTGTATGGATAGATACTTTGGGGGT30950.13052947309110663No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCCTGTCTCTTATAC30470.1285051064648148No Hit
AAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGG30470.1285051064648148No Hit
CCCTCTAATTATCCTAAGATAAAATCCAATCTGGCTCACGCCGATTTGAAC30200.12736640023752568No Hit
TTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCTTAT29850.12589029957252124No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCT29820.125763776658378No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGCTGTCTCT29690.12521551069709067No Hit
AAGCAGGCATTTCGCCTAAATATTCTTGCATGGAATAATGGAATAAGATCT28740.12120895174922146No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGCTGTCTCTTATACAC28610.1206606857879341No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTGTCTCTTATAC27550.11619020948820638No Hit
TAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTA27120.11437671438548663No Hit
CTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGTTCAAATCCT26700.11260539358748131No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATCTGTCTCT26610.11222582484505161No Hit
GTATTTTGACTGTGCTAAGGTAGCGAAGTCATTTGTTTCTTTATTGGGAAC26610.11222582484505161No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCCTGTCTCT26450.111551035969621No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCT26360.1111714672271913No Hit
ATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAAACTAATAGTCTGT25290.10665881662274916No Hit
ATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAAT25160.1061105506614618No Hit
ATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGG24370.1027787805890232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6650.036.87833445
GTTTTAT17950.032.518151
GCCCAAC352.7944185E-432.19531
TTGCGAA17900.031.29772610
ATGCCGT7850.030.95426844
TGCGAAC18150.030.4947411
CGAACTT18250.030.32764613
TATGCCG8150.030.09091243
TTATTAT18850.029.6010274
TATTATT19400.028.8777965
CTCGTAT8450.028.75633239
TCGTATG8500.028.58717740
ACGAGAC8700.028.44722222
CGAGCAT406.16943E-428.12395711
GTATGCC8650.028.09144642
TATCTCG8700.027.9336
CCACGAG8950.027.90399620
GACTAGA8800.027.86828626
CCCACGA8900.027.8079619
ATTGCGA20350.027.3085699
Produced by FastQC (version 0.11.2)