FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_14.3410000000a001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_14.3410000000a001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418107
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC21360.15062333096162703TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3800.027.82886143
CGTATGC3900.027.69124441
TCGCGCG3450.027.3902539
GCCCACG3900.027.11434418
CGCGCGC3550.026.61869810
GTATGCC4000.026.43648542
GGTCGCG3700.025.539567
CCCACGA4150.025.4809519
GCGCGCT4750.024.63063211
ATGCCGT4300.024.59294544
GTCGCGC4000.024.1865738
TCGTATG4650.022.74106240
GCGCCAT4300.021.975921
CTCGTAT5000.021.14918739
AGCGCCA4500.020.99919120
CGCGCTT5300.020.80108612
GGGGCTA4600.020.54269236
GGCTAGT4900.020.20330638
CTAGACT1452.1827873E-1020.1716384
CACGAGA5400.019.99923321
Produced by FastQC (version 0.11.2)