FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_13.34100000009fe9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_13.34100000009fe9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432769
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.24978683778181893No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC7580.17515117764904603RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6540.1511198815072244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.14280135591967077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.12292932257162598No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5050.11669042838096073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1050.032.1416241
TATGCCG1050.032.1416243
GTATGCC1150.029.34669542
ATGCCGT1300.027.6912444
CTCGTAT1250.026.99895939
TCGTATG1250.026.99895940
CGAGACG1250.026.99895923
GCCCACG1300.025.96053918
TATCTCG1400.025.71329536
GTATAAG551.3506351E-424.5871221
ATACCGA551.3666357E-424.5445086
ACGAATT1301.8189894E-1224.22983627
ATCTCGT1500.023.99907737
CACGAGA1500.023.99907721
GAATTGC1353.6379788E-1223.33243629
GACGAAT1353.6379788E-1223.33243626
CCCACGA1450.023.27496519
CGAATTG1600.022.49913428
TATAAGG602.4647047E-422.4991342
TGCCGTC1700.022.49913445
Produced by FastQC (version 0.11.2)