FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_12.34100000009fdc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_12.34100000009fdc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC21.7543859649122806No Hit
CACCACCAGTACTCCTGAAGAGTCTCACCTTTCCAGGCGAAAACAGCGGCG10.8771929824561403No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTGATACCCCCCCTACTCC10.8771929824561403No Hit
TATAAAGGAAGGGTGATGATCATTTCTTGGGTTGGAACTTGGATTTGATGT10.8771929824561403No Hit
ATGCCGAGGCACGCCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCG10.8771929824561403No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.8771929824561403No Hit
ATCAAGATGGTAGCAAGAAAGAAGCTATTCCTGTCTCTTATACACATCTCC10.8771929824561403No Hit
CTTATGTAGCCACCAAAGGCAATTGCAAAAATTAACCCTTGATCATCATAT10.8771929824561403No Hit
CTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCT10.8771929824561403No Hit
CTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGG10.8771929824561403No Hit
CATGAGTAGCGTGTGACGGGTGGGAATCCCGTCCGCCGATTATCCAAGGGT10.8771929824561403No Hit
GTGTAAGCAAGAGTGAGTTACTTGACCGTGAAGCTGAAGATCCTGCTGTAC10.8771929824561403No Hit
GTATTAGTACTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCCGTCTT10.8771929824561403No Hit
GCTTAAATATGTTGAAGAGGAGAATTTGGGACAGATGGTCCATTGATTCAT10.8771929824561403No Hit
CGTCTTTGCCGCGTCAAGGCTCGGGAAATACGTCGGCTCCATAAATATCTG10.8771929824561403No Hit
GTCTCTACTTACTTGAAGTTGACATCAATCACTCACGTCTGTTGTTGTATT10.8771929824561403No Hit
GCTTAGCGAGAATAATCGCTCCTCTAATGGCCGTAGCAACTTCCTTAGAGC10.8771929824561403No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTATACCATCTC10.8771929824561403No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGATTTTAAGAAAAAGAAAGA10.8771929824561403No Hit
GTGGGATTCTCGCTGTGTTAGGCTGCGTCGATAACTCTCAAGCTAAACGTT10.8771929824561403No Hit
TTTCTTAACCCAAGCAATCAGTTTCGGAACCTCAGATTCGATACTGAAGTT10.8771929824561403No Hit
AACTTCGAATTGGGAGGGCGATCCTCCCGGTGAACTGACCGTACCCCAAAC10.8771929824561403No Hit
AAACAACGGCGGAGATAGAATTCGGTCTGCGGAGGAGGAAGCGGAAGCGAA10.8771929824561403No Hit
GGATTAACGAGATTCCCACTGTCCCTGTCTACTATCCAGCGAAACCACAGC10.8771929824561403No Hit
CTGTAACCTCTGCCGATGATTCCTCTGTTGCTACCTTTGGCACCTCCTGTT10.8771929824561403No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTGGTTTTTACCCCCCCCTTTA10.8771929824561403No Hit
CCGCCAGCCTGATGGACAGCGAGCGCCAGGCGTTTTCGCCCATGCCTGTCT10.8771929824561403No Hit
GTGGTGGCAACGTTGGTGACTCTGTCACTATTCCTCTGCCTCAGTGGGTTG10.8771929824561403No Hit
CGACCAGTGAGCTATTACGCACTCTTTGAAGGGTGGCTGCTTCTAAGCCAA10.8771929824561403No Hit
GCGTAACCCACGTCCACCTTGCATTCCAAAGTCAGTTGGTTTTCAGAGGTT10.8771929824561403No Hit
GTTATTTGTAACGTTGAACATCATGTTGGTGATCGTGGTGTTTTCGCTAGA10.8771929824561403No Hit
TAATAAAATAGGACGTGCGGTTCTATTTTGTTGGTTTTTGGAATCGCCGTA10.8771929824561403No Hit
GTGTGTGTGGCTGCCGTCGCATGCTGTTGATGTTGTCGTTAGCGGAAGTCG10.8771929824561403No Hit
TCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGAAG10.8771929824561403No Hit
AACAATGTACCTTCTTGTCCAGTGATATCAAATTGATATGTGAAGTTGCTG10.8771929824561403No Hit
GACTAAACCTGTAAAACATGCTTCTACAGTCTGCATGCCTCAGCCCTCAGG10.8771929824561403No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC10.8771929824561403No Hit
GAGTGGAGTGCTGGTGTTATCCTCTACATTTTACTCAGCGGTGTTCCTCCC10.8771929824561403No Hit
ATTCTACACTTAGCATCCTTGCCCCACGGTCCAAGGGCAGGAGTCACCCTG10.8771929824561403No Hit
CCTTGGACGCGCCGGGCAGTTCCTTATCGACCATCGCCACCACTTCCGGCC10.8771929824561403No Hit
GATCTTCAGATGGAGAGGAAAGCAATGGAGATGAAGTAGAGACTGATGATG10.8771929824561403No Hit
CTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAG10.8771929824561403No Hit
GGAATGCACGCATTAATAATCTATGATGATCTTAGTAAACAGGCGGTGGCA10.8771929824561403No Hit
GGGTAGATCACCAGGTTTCGCGTCTACTACTACCAACTATTGTCGCCCTAT10.8771929824561403No Hit
TCTTTAAACCTCCAAAGAACAAATCTTCAAGTGGTGGTTTCTCACTTTCTA10.8771929824561403No Hit
GGCTTAAGCCGCTCTTCCCATTTCCTCGCTGACCCATCATGCAAAAGGTAC10.8771929824561403No Hit
ACCCGGTGTTGCTGAAGGTGTGAAAGACGAGATTTTTCTTTTGCTCTTCAT10.8771929824561403No Hit
GGGTCATTCTATGAAGAAGAAGAGGAAGAGGATGATGGTGCAAAAGGTAGT10.8771929824561403No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATG10.8771929824561403No Hit
TCCCATTTCCTCGCTGACCCATCATGCAAAAGGTACGCCGTTAGAGCTGTC10.8771929824561403No Hit
CTCTCTTGGAGCGAATTGGATGATCGGGCCGAGGGCAGCGTAGCGCCTCTT10.8771929824561403No Hit
GGGAGCTTTCCCCGGGCGTCGAACAGTCAGCTCAGAACTGGTACGGACAAG10.8771929824561403No Hit
GCTACAAATCTCTCATTTCACCATCTAAACATTTTACATTCACTTTTTCCA10.8771929824561403No Hit
CCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGG10.8771929824561403No Hit
TTCCGCTACTGACACTTGAAAAAGCAGCTTTCAAGGTCTCGTCGCTTCGGT10.8771929824561403No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGTTCCCCCCCCG10.8771929824561403No Hit
GAGTTACTTCGTACCTTGACTGAAGATTCCTAAAGGGCGCTGTGAACTGTA10.8771929824561403No Hit
GTGCATTGCACGGTGGATGTGAAGAAGTAGGCCATTATCTCGGCAATAATG10.8771929824561403No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTT10.8771929824561403No Hit
GGGCCAGGTTTCCCCATTCGGACATCCTCGGATCAACGCTTTATTGACAAC10.8771929824561403No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTCCA10.8771929824561403No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCGGGGTTTAACCCCT10.8771929824561403No Hit
ATATATGGGAATGCCAATGAGAAGTTGGAAGCTGTAAATAGATTATTCAAT10.8771929824561403No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC10.8771929824561403No Hit
CCTTCGGAATTTGAAGCTAGAGGTGTCAGAAAAGTTACCACAGGGATAACT10.8771929824561403No Hit
CTCCTCATCTTCCTCGGTCTTTTCTTGAAGCTCCTCTAGCAGAGTAATCTT10.8771929824561403No Hit
GCTCGTAAGCCCTGGATCGCTGTTGGAATGAGGAACGTTGACTACAAGAGA10.8771929824561403No Hit
ACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAG10.8771929824561403No Hit
TAATAAATCCCATGTTTGTGTTACTTCCGGTTGCACCCTTTTAGTTTGGTA10.8771929824561403No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAG10.8771929824561403No Hit
GGCTCTGACCGCGTGCCTGTTGAAGAATGAGCCGGCGACTCATAGGCAGTG10.8771929824561403No Hit
CCTGGAGATAGCTAGTTCTCCCCGAAATGTATTGAGGCTGTCTCTTATACA10.8771929824561403No Hit
GTTGTGATGTTCATAGCAAGTTTATCCATATTCTTGCGTATAAGCTCTTGA10.8771929824561403No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTGAAAACCAC10.8771929824561403No Hit
GGTGAAAAGAACCCTATGACGGGAGTGCAATAGAGAACCTGAGATCCGATG10.8771929824561403No Hit
GCTAAATACTCCTGGGTGACCGATAGCGAAGTAGTACCGTGAGGGAAGGGT10.8771929824561403No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA10.8771929824561403No Hit
AGGTTCGGGAATGAAGATCTCGATCAGGCAGTTTGGACCTGGAATGATGCA10.8771929824561403No Hit
CCTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAG10.8771929824561403No Hit
GCCCAATGGTGGCTTGATGATGCCGTATGATCTAAAGGAGCTTGTCAAAGG10.8771929824561403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGT10.8771929824561403No Hit
CGTCCCTCTCTCTTCGATGCAGAACCCGCAAAAAACTCATCCACACTCAAA10.8771929824561403No Hit
GGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCTGTC10.8771929824561403No Hit
GGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCAC10.8771929824561403No Hit
GTATGCTCTGAGGCTAAAGATCTTGTTGAGATGGGGAAGGTCCAACTGGCT10.8771929824561403No Hit
ATCTTCCTCTTCCAATCCATATCAACCATTTTTATTTTTTTAAGGTCTGTG10.8771929824561403No Hit
GTGTGAATATGAATCACGTAAAGGTAGAAGAGAAGATGTGTGCAGCTGTTT10.8771929824561403No Hit
CAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGTACTCTG10.8771929824561403No Hit
GTGGTGATCGTCTCACTCGTGCCTCCAAGGTGTTGGAACAGCTCAGTGGTC10.8771929824561403No Hit
ATCGTACGCTAAGGTCCCTAAGCAATCACTTAGTGGAAAAGGAAGTGATCG10.8771929824561403No Hit
ATTCGACGAAGTTTTTTAAGAGAGAAGAAGGAATCTTCAGATGGGAATAGC10.8771929824561403No Hit
ATTCTATTGAGTCTGACTCATAGTGATCATTTATCAAAGAATGACTCTGGT10.8771929824561403No Hit
TATTAACAGCTACTCTAGGAGGAATGTTTTTATGTGGTGCTAACGATTTAA10.8771929824561403No Hit
AAAGAGGAGCCCACCATGAAACCGACGAAACAGGTTTCTTACGGGCTGGAT10.8771929824561403No Hit
GCTCGATAATGATCTTCCAAAACCATGTTAGCACCAGTCATGGTTCCAATG10.8771929824561403No Hit
CCTTTCTATCACTTGAAGATTATCTACGCAATTCGTAAATGATCAAACTAG10.8771929824561403No Hit
ATGGAACACTTTCTCCAATAGTCGATGACCCTGATCGCGCCATCATGTCTG10.8771929824561403No Hit
CATTACATTCATTTCCGAACGTAGGAAGAAACTTGGGTTGGAGTAAATCTC10.8771929824561403No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTATACC10.8771929824561403No Hit
ACTTTAGTCCGGCGGTTTCTCAATCTGGTGATTTCTTAGCCGTTGCTTCTT10.8771929824561403No Hit
CAAGGATACTTTAAACGTCCTGAAATCACAGAGGAGGCAATCACGAAGGAT10.8771929824561403No Hit
AGAGTGGGCGATGGCGAGTTTGTTGAACCACCCAAAAGTTTTGGACAAAGT10.8771929824561403No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10.8771929824561403TruSeq Adapter, Index 12 (95% over 21bp)
CAAAGAGGGTATTCCTCCGGACCAGCAGAGATTGATCTTCGCCGAAAAACA10.8771929824561403No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10.8771929824561403No Hit
GGCATAGCGAGATCAAAGTGCTCACGAGGAGGATTACACCAACCACCATTG10.8771929824561403No Hit
CGTTTGATCGTTGCTATCAGTTCTAACAATATCAAATAAAAATTTGAAAGT10.8771929824561403No Hit
ACTTAATCTTGATTCGATTGAACACTTAGCGTGAATGATAAAGAGAAACTA10.8771929824561403No Hit
TCTCCAGAGATCTGAATCTCTTTCCGATAATCCATCTCTCACTCTTCTACC10.8771929824561403No Hit
ATCATTGACTTGGTGAGCCGTTACCTCACCTGTCTCTTATACACATCTCCC10.8771929824561403No Hit
TGCCAAGGGTGCTGCTAACTTCACCTCCCAGGTCATCATCATGAACCACCC10.8771929824561403No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8771929824561403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10.8771929824561403No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers