##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_12.34100000009fdc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 114 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.36842105263158 38.0 32.0 38.0 32.0 38.0 2 34.95614035087719 38.0 32.0 38.0 27.0 38.0 3 35.728070175438596 38.0 32.0 38.0 32.0 38.0 4 36.05263157894737 38.0 32.0 38.0 32.0 38.0 5 36.37719298245614 38.0 38.0 38.0 32.0 38.0 6 37.71052631578947 38.0 38.0 40.0 32.0 40.0 7 37.824561403508774 38.0 38.0 40.0 32.0 40.0 8 37.96491228070175 38.0 38.0 40.0 38.0 40.0 9 37.675438596491226 40.0 38.0 40.0 32.0 40.0 10 37.58771929824562 40.0 38.0 40.0 32.0 40.0 11 37.473684210526315 40.0 38.0 40.0 32.0 40.0 12 37.71052631578947 40.0 38.0 40.0 32.0 40.0 13 37.30701754385965 40.0 38.0 40.0 32.0 40.0 14 38.21052631578947 40.0 38.0 40.0 32.0 40.0 15 37.64035087719298 40.0 38.0 40.0 32.0 40.0 16 37.35087719298246 40.0 38.0 40.0 32.0 40.0 17 37.94736842105263 40.0 38.0 40.0 32.0 40.0 18 38.18421052631579 40.0 38.0 40.0 32.0 40.0 19 37.219298245614034 40.0 38.0 40.0 32.0 40.0 20 37.728070175438596 40.0 38.0 40.0 32.0 40.0 21 37.37719298245614 40.0 38.0 40.0 32.0 40.0 22 37.62280701754386 40.0 38.0 40.0 32.0 40.0 23 37.59649122807018 40.0 38.0 40.0 32.0 40.0 24 37.64035087719298 40.0 38.0 40.0 32.0 40.0 25 37.94736842105263 40.0 38.0 40.0 32.0 40.0 26 37.44736842105263 38.0 38.0 40.0 32.0 40.0 27 38.00877192982456 38.0 38.0 40.0 32.0 40.0 28 37.333333333333336 40.0 38.0 40.0 32.0 40.0 29 37.49122807017544 38.0 38.0 40.0 32.0 40.0 30 37.04385964912281 38.0 38.0 40.0 32.0 40.0 31 37.06140350877193 38.0 38.0 40.0 32.0 40.0 32 36.46491228070175 38.0 38.0 40.0 27.0 40.0 33 36.3859649122807 38.0 38.0 40.0 32.0 40.0 34 36.675438596491226 38.0 38.0 40.0 27.0 40.0 35 35.93859649122807 38.0 38.0 40.0 27.0 40.0 36 36.526315789473685 38.0 38.0 40.0 32.0 40.0 37 36.64912280701754 38.0 38.0 40.0 27.0 40.0 38 37.24561403508772 38.0 38.0 40.0 32.0 40.0 39 36.85964912280702 38.0 38.0 40.0 32.0 40.0 40 36.86842105263158 38.0 38.0 40.0 32.0 40.0 41 36.19298245614035 38.0 38.0 40.0 32.0 40.0 42 36.26315789473684 38.0 38.0 40.0 27.0 40.0 43 35.771929824561404 38.0 38.0 40.0 27.0 40.0 44 35.54385964912281 38.0 38.0 40.0 13.0 40.0 45 35.96491228070175 38.0 38.0 40.0 27.0 40.0 46 35.333333333333336 38.0 38.0 40.0 13.0 40.0 47 35.46491228070175 38.0 32.0 40.0 27.0 40.0 48 35.81578947368421 38.0 38.0 40.0 27.0 40.0 49 35.728070175438596 38.0 38.0 40.0 27.0 40.0 50 36.08771929824562 38.0 38.0 40.0 27.0 40.0 51 35.14035087719298 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1203 1 -4.909090909090909 1203 2 1.0909090909090908 1203 3 1.0909090909090908 1203 4 0.5454545454545459 1203 5 -4.909090909090909 1203 6 NaN 1203 7 NaN 1203 8 NaN 1203 9 NaN 1203 10 -1.454545454545455 1203 11 NaN 1203 12 NaN 1203 13 -1.454545454545455 1203 14 -6.7272727272727275 1203 15 -1.454545454545455 1203 16 NaN 1203 17 -1.6363636363636367 1203 18 -1.0909090909090917 1203 19 -0.7272727272727275 1203 20 -1.454545454545455 1203 21 0.9090909090909083 1203 22 -1.2727272727272734 1203 23 -1.2727272727272734 1203 24 -1.2727272727272734 1203 25 -6.909090909090909 1203 26 -6.7272727272727275 1203 27 NaN 1203 28 NaN 1203 29 -1.2727272727272734 1203 30 -6.0 1203 31 NaN 1203 32 NaN 1203 33 NaN 1203 34 NaN 1203 35 NaN 1203 36 NaN 1203 37 NaN 1203 38 NaN 1203 39 NaN 1203 40 NaN 1203 41 NaN 1203 42 NaN 1203 43 NaN 1203 44 NaN 1203 45 NaN 1203 46 NaN 1203 47 NaN 1203 48 NaN 1203 49 NaN 1203 50 NaN 1203 51 NaN 2202 1 -4.909090909090909 2202 2 -4.909090909090909 2202 3 -4.909090909090909 2202 4 0.5454545454545459 2202 5 -4.909090909090909 2202 6 NaN 2202 7 NaN 2202 8 NaN 2202 9 NaN 2202 10 -1.454545454545455 2202 11 NaN 2202 12 NaN 2202 13 0.545454545454545 2202 14 -0.7272727272727275 2202 15 0.545454545454545 2202 16 NaN 2202 17 0.3636363636363633 2202 18 0.9090909090909083 2202 19 -6.7272727272727275 2202 20 -1.454545454545455 2202 21 -1.0909090909090917 2202 22 -1.2727272727272734 2202 23 -1.2727272727272734 2202 24 -1.2727272727272734 2202 25 -0.9090909090909092 2202 26 -0.7272727272727275 2202 27 NaN 2202 28 NaN 2202 29 -1.2727272727272734 2202 30 0.0 2202 31 NaN 2202 32 NaN 2202 33 NaN 2202 34 NaN 2202 35 NaN 2202 36 NaN 2202 37 NaN 2202 38 NaN 2202 39 NaN 2202 40 NaN 2202 41 NaN 2202 42 NaN 2202 43 NaN 2202 44 NaN 2202 45 NaN 2202 46 NaN 2202 47 NaN 2202 48 NaN 2202 49 NaN 2202 50 NaN 2202 51 NaN 2112 1 1.0909090909090908 2112 2 1.0909090909090908 2112 3 1.0909090909090908 2112 4 0.5454545454545459 2112 5 1.0909090909090908 2112 6 NaN 2112 7 NaN 2112 8 NaN 2112 9 NaN 2112 10 0.545454545454545 2112 11 NaN 2112 12 NaN 2112 13 0.545454545454545 2112 14 1.2727272727272725 2112 15 -1.454545454545455 2112 16 NaN 2112 17 0.3636363636363633 2112 18 0.9090909090909083 2112 19 1.2727272727272725 2112 20 0.545454545454545 2112 21 0.9090909090909083 2112 22 0.7272727272727266 2112 23 0.7272727272727266 2112 24 0.7272727272727266 2112 25 1.0909090909090908 2112 26 -0.7272727272727275 2112 27 NaN 2112 28 NaN 2112 29 0.7272727272727266 2112 30 2.0 2112 31 NaN 2112 32 NaN 2112 33 NaN 2112 34 NaN 2112 35 NaN 2112 36 NaN 2112 37 NaN 2112 38 NaN 2112 39 NaN 2112 40 NaN 2112 41 NaN 2112 42 NaN 2112 43 NaN 2112 44 NaN 2112 45 NaN 2112 46 NaN 2112 47 NaN 2112 48 NaN 2112 49 NaN 2112 50 NaN 2112 51 NaN 2101 1 1.0909090909090908 2101 2 -4.909090909090909 2101 3 1.0909090909090908 2101 4 -5.454545454545454 2101 5 1.0909090909090908 2101 6 NaN 2101 7 NaN 2101 8 NaN 2101 9 NaN 2101 10 0.545454545454545 2101 11 NaN 2101 12 NaN 2101 13 -1.454545454545455 2101 14 -0.7272727272727275 2101 15 0.545454545454545 2101 16 NaN 2101 17 0.3636363636363633 2101 18 -1.0909090909090917 2101 19 -0.7272727272727275 2101 20 -1.454545454545455 2101 21 -1.0909090909090917 2101 22 0.7272727272727266 2101 23 0.7272727272727266 2101 24 0.7272727272727266 2101 25 1.0909090909090908 2101 26 1.2727272727272725 2101 27 NaN 2101 28 NaN 2101 29 -1.2727272727272734 2101 30 -6.0 2101 31 NaN 2101 32 NaN 2101 33 NaN 2101 34 NaN 2101 35 NaN 2101 36 NaN 2101 37 NaN 2101 38 NaN 2101 39 NaN 2101 40 NaN 2101 41 NaN 2101 42 NaN 2101 43 NaN 2101 44 NaN 2101 45 NaN 2101 46 NaN 2101 47 NaN 2101 48 NaN 2101 49 NaN 2101 50 NaN 2101 51 NaN 1208 1 1.0909090909090908 1208 2 1.0909090909090908 1208 3 1.0909090909090908 1208 4 0.5454545454545459 1208 5 1.0909090909090908 1208 6 NaN 1208 7 NaN 1208 8 NaN 1208 9 NaN 1208 10 0.545454545454545 1208 11 NaN 1208 12 NaN 1208 13 0.545454545454545 1208 14 1.2727272727272725 1208 15 0.545454545454545 1208 16 NaN 1208 17 0.3636363636363633 1208 18 0.9090909090909083 1208 19 1.2727272727272725 1208 20 0.545454545454545 1208 21 -1.0909090909090917 1208 22 -1.2727272727272734 1208 23 0.7272727272727266 1208 24 0.7272727272727266 1208 25 1.0909090909090908 1208 26 1.2727272727272725 1208 27 NaN 1208 28 NaN 1208 29 0.7272727272727266 1208 30 2.0 1208 31 NaN 1208 32 NaN 1208 33 NaN 1208 34 NaN 1208 35 NaN 1208 36 NaN 1208 37 NaN 1208 38 NaN 1208 39 NaN 1208 40 NaN 1208 41 NaN 1208 42 NaN 1208 43 NaN 1208 44 NaN 1208 45 NaN 1208 46 NaN 1208 47 NaN 1208 48 NaN 1208 49 NaN 1208 50 NaN 1208 51 NaN 1111 1 1.0909090909090908 1111 2 1.0909090909090908 1111 3 1.0909090909090908 1111 4 0.5454545454545459 1111 5 1.0909090909090908 1111 6 NaN 1111 7 NaN 1111 8 NaN 1111 9 NaN 1111 10 0.545454545454545 1111 11 NaN 1111 12 NaN 1111 13 0.545454545454545 1111 14 1.2727272727272725 1111 15 0.545454545454545 1111 16 NaN 1111 17 0.3636363636363633 1111 18 0.9090909090909083 1111 19 1.2727272727272725 1111 20 0.545454545454545 1111 21 0.9090909090909083 1111 22 0.7272727272727266 1111 23 0.7272727272727266 1111 24 0.7272727272727266 1111 25 1.0909090909090908 1111 26 1.2727272727272725 1111 27 NaN 1111 28 NaN 1111 29 0.7272727272727266 1111 30 2.0 1111 31 NaN 1111 32 NaN 1111 33 NaN 1111 34 NaN 1111 35 NaN 1111 36 NaN 1111 37 NaN 1111 38 NaN 1111 39 NaN 1111 40 NaN 1111 41 NaN 1111 42 NaN 1111 43 NaN 1111 44 NaN 1111 45 NaN 1111 46 NaN 1111 47 NaN 1111 48 NaN 1111 49 NaN 1111 50 NaN 1111 51 NaN 2208 1 1.0909090909090908 2208 2 1.0909090909090908 2208 3 1.0909090909090908 2208 4 0.5454545454545459 2208 5 1.0909090909090908 2208 6 NaN 2208 7 NaN 2208 8 NaN 2208 9 NaN 2208 10 -1.454545454545455 2208 11 NaN 2208 12 NaN 2208 13 -1.454545454545455 2208 14 -0.7272727272727275 2208 15 -1.454545454545455 2208 16 NaN 2208 17 -1.6363636363636367 2208 18 -1.0909090909090917 2208 19 -0.7272727272727275 2208 20 0.545454545454545 2208 21 -1.0909090909090917 2208 22 -1.2727272727272734 2208 23 -1.2727272727272734 2208 24 -1.2727272727272734 2208 25 -0.9090909090909092 2208 26 -0.7272727272727275 2208 27 NaN 2208 28 NaN 2208 29 -1.2727272727272734 2208 30 0.0 2208 31 NaN 2208 32 NaN 2208 33 NaN 2208 34 NaN 2208 35 NaN 2208 36 NaN 2208 37 NaN 2208 38 NaN 2208 39 NaN 2208 40 NaN 2208 41 NaN 2208 42 NaN 2208 43 NaN 2208 44 NaN 2208 45 NaN 2208 46 NaN 2208 47 NaN 2208 48 NaN 2208 49 NaN 2208 50 NaN 2208 51 NaN 1105 1 1.0909090909090908 1105 2 1.0909090909090908 1105 3 1.0909090909090908 1105 4 0.5454545454545459 1105 5 1.0909090909090908 1105 6 NaN 1105 7 NaN 1105 8 NaN 1105 9 NaN 1105 10 0.545454545454545 1105 11 NaN 1105 12 NaN 1105 13 0.545454545454545 1105 14 1.2727272727272725 1105 15 0.545454545454545 1105 16 NaN 1105 17 0.3636363636363633 1105 18 0.9090909090909083 1105 19 1.2727272727272725 1105 20 0.545454545454545 1105 21 0.9090909090909083 1105 22 0.7272727272727266 1105 23 0.7272727272727266 1105 24 0.7272727272727266 1105 25 1.0909090909090908 1105 26 1.2727272727272725 1105 27 NaN 1105 28 NaN 1105 29 0.7272727272727266 1105 30 2.0 1105 31 NaN 1105 32 NaN 1105 33 NaN 1105 34 NaN 1105 35 NaN 1105 36 NaN 1105 37 NaN 1105 38 NaN 1105 39 NaN 1105 40 NaN 1105 41 NaN 1105 42 NaN 1105 43 NaN 1105 44 NaN 1105 45 NaN 1105 46 NaN 1105 47 NaN 1105 48 NaN 1105 49 NaN 1105 50 NaN 1105 51 NaN 2214 1 1.0909090909090908 2214 2 1.0909090909090908 2214 3 -4.909090909090909 2214 4 0.5454545454545459 2214 5 1.0909090909090908 2214 6 NaN 2214 7 NaN 2214 8 NaN 2214 9 NaN 2214 10 0.545454545454545 2214 11 NaN 2214 12 NaN 2214 13 0.545454545454545 2214 14 1.2727272727272725 2214 15 0.545454545454545 2214 16 NaN 2214 17 0.3636363636363633 2214 18 0.9090909090909083 2214 19 1.2727272727272725 2214 20 0.545454545454545 2214 21 0.9090909090909083 2214 22 0.7272727272727266 2214 23 0.7272727272727266 2214 24 0.7272727272727266 2214 25 1.0909090909090908 2214 26 1.2727272727272725 2214 27 NaN 2214 28 NaN 2214 29 0.7272727272727266 2214 30 0.0 2214 31 NaN 2214 32 NaN 2214 33 NaN 2214 34 NaN 2214 35 NaN 2214 36 NaN 2214 37 NaN 2214 38 NaN 2214 39 NaN 2214 40 NaN 2214 41 NaN 2214 42 NaN 2214 43 NaN 2214 44 NaN 2214 45 NaN 2214 46 NaN 2214 47 NaN 2214 48 NaN 2214 49 NaN 2214 50 NaN 2214 51 NaN 2110 1 1.0909090909090908 2110 2 1.0909090909090908 2110 3 1.0909090909090908 2110 4 0.5454545454545459 2110 5 1.0909090909090908 2110 6 NaN 2110 7 NaN 2110 8 NaN 2110 9 NaN 2110 10 0.545454545454545 2110 11 NaN 2110 12 NaN 2110 13 0.545454545454545 2110 14 1.2727272727272725 2110 15 0.545454545454545 2110 16 NaN 2110 17 0.3636363636363633 2110 18 -1.0909090909090917 2110 19 1.2727272727272725 2110 20 0.545454545454545 2110 21 0.9090909090909083 2110 22 0.7272727272727266 2110 23 -1.2727272727272734 2110 24 0.7272727272727266 2110 25 1.0909090909090908 2110 26 1.2727272727272725 2110 27 NaN 2110 28 NaN 2110 29 0.7272727272727266 2110 30 2.0 2110 31 NaN 2110 32 NaN 2110 33 NaN 2110 34 NaN 2110 35 NaN 2110 36 NaN 2110 37 NaN 2110 38 NaN 2110 39 NaN 2110 40 NaN 2110 41 NaN 2110 42 NaN 2110 43 NaN 2110 44 NaN 2110 45 NaN 2110 46 NaN 2110 47 NaN 2110 48 NaN 2110 49 NaN 2110 50 NaN 2110 51 NaN 2108 1 1.0909090909090908 2108 2 1.0909090909090908 2108 3 1.0909090909090908 2108 4 0.5454545454545459 2108 5 1.0909090909090908 2108 6 NaN 2108 7 NaN 2108 8 NaN 2108 9 NaN 2108 10 0.545454545454545 2108 11 NaN 2108 12 NaN 2108 13 0.545454545454545 2108 14 1.2727272727272725 2108 15 0.545454545454545 2108 16 NaN 2108 17 0.3636363636363633 2108 18 -1.0909090909090917 2108 19 1.2727272727272725 2108 20 0.545454545454545 2108 21 -1.0909090909090917 2108 22 0.7272727272727266 2108 23 0.7272727272727266 2108 24 -1.2727272727272734 2108 25 1.0909090909090908 2108 26 1.2727272727272725 2108 27 NaN 2108 28 NaN 2108 29 0.7272727272727266 2108 30 2.0 2108 31 NaN 2108 32 NaN 2108 33 NaN 2108 34 NaN 2108 35 NaN 2108 36 NaN 2108 37 NaN 2108 38 NaN 2108 39 NaN 2108 40 NaN 2108 41 NaN 2108 42 NaN 2108 43 NaN 2108 44 NaN 2108 45 NaN 2108 46 NaN 2108 47 NaN 2108 48 NaN 2108 49 NaN 2108 50 NaN 2108 51 NaN >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 1.0 27 1.0 28 0.0 29 1.0 30 4.0 31 2.0 32 7.0 33 8.0 34 6.0 35 9.0 36 9.0 37 2.0 38 22.0 39 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.35087719298245 20.175438596491226 12.280701754385964 27.192982456140353 2 20.175438596491226 21.052631578947366 35.96491228070175 22.807017543859647 3 25.438596491228072 14.912280701754385 36.84210526315789 22.807017543859647 4 14.035087719298245 17.543859649122805 42.10526315789473 26.31578947368421 5 16.666666666666664 37.719298245614034 33.33333333333333 12.280701754385964 6 23.684210526315788 44.73684210526316 14.912280701754385 16.666666666666664 7 24.561403508771928 27.192982456140353 28.07017543859649 20.175438596491226 8 23.684210526315788 30.701754385964914 26.31578947368421 19.298245614035086 9 28.947368421052634 14.035087719298245 22.807017543859647 34.21052631578947 10 14.035087719298245 27.192982456140353 34.21052631578947 24.561403508771928 11 29.82456140350877 19.298245614035086 28.947368421052634 21.929824561403507 12 19.298245614035086 21.052631578947366 27.192982456140353 32.45614035087719 13 28.947368421052634 9.649122807017543 39.473684210526315 21.929824561403507 14 21.052631578947366 19.298245614035086 29.82456140350877 29.82456140350877 15 25.438596491228072 28.07017543859649 18.421052631578945 28.07017543859649 16 28.07017543859649 33.33333333333333 29.82456140350877 8.771929824561402 17 16.666666666666664 34.21052631578947 28.947368421052634 20.175438596491226 18 28.07017543859649 22.807017543859647 28.07017543859649 21.052631578947366 19 23.684210526315788 27.192982456140353 25.438596491228072 23.684210526315788 20 20.175438596491226 23.684210526315788 29.82456140350877 26.31578947368421 21 22.807017543859647 24.561403508771928 35.08771929824561 17.543859649122805 22 28.947368421052634 21.052631578947366 28.947368421052634 21.052631578947366 23 12.280701754385964 31.57894736842105 28.947368421052634 27.192982456140353 24 21.929824561403507 26.31578947368421 29.82456140350877 21.929824561403507 25 21.929824561403507 26.31578947368421 30.701754385964914 21.052631578947366 26 14.912280701754385 28.07017543859649 39.473684210526315 17.543859649122805 27 20.175438596491226 28.947368421052634 25.438596491228072 25.438596491228072 28 21.052631578947366 29.82456140350877 29.82456140350877 19.298245614035086 29 21.929824561403507 29.82456140350877 26.31578947368421 21.929824561403507 30 28.07017543859649 33.33333333333333 23.684210526315788 14.912280701754385 31 26.31578947368421 28.07017543859649 26.31578947368421 19.298245614035086 32 14.912280701754385 26.31578947368421 41.228070175438596 17.543859649122805 33 21.052631578947366 23.684210526315788 37.719298245614034 17.543859649122805 34 18.421052631578945 28.07017543859649 30.701754385964914 22.807017543859647 35 23.684210526315788 27.192982456140353 32.45614035087719 16.666666666666664 36 24.561403508771928 25.438596491228072 33.33333333333333 16.666666666666664 37 22.807017543859647 26.31578947368421 28.947368421052634 21.929824561403507 38 17.543859649122805 26.31578947368421 28.07017543859649 28.07017543859649 39 18.421052631578945 23.684210526315788 32.45614035087719 25.438596491228072 40 21.929824561403507 22.807017543859647 32.45614035087719 22.807017543859647 41 24.561403508771928 21.929824561403507 30.701754385964914 22.807017543859647 42 19.298245614035086 33.33333333333333 26.31578947368421 21.052631578947366 43 21.929824561403507 28.947368421052634 28.07017543859649 21.052631578947366 44 21.929824561403507 23.684210526315788 22.807017543859647 31.57894736842105 45 21.929824561403507 28.947368421052634 28.947368421052634 20.175438596491226 46 19.298245614035086 28.07017543859649 26.31578947368421 26.31578947368421 47 12.280701754385964 29.82456140350877 27.192982456140353 30.701754385964914 48 20.175438596491226 21.929824561403507 35.08771929824561 22.807017543859647 49 28.947368421052634 26.31578947368421 19.298245614035086 25.438596491228072 50 18.421052631578945 23.684210526315788 36.84210526315789 21.052631578947366 51 27.192982456140353 20.175438596491226 28.07017543859649 24.561403508771928 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.5 16 1.0 17 1.5 18 2.0 19 2.0 20 2.0 21 1.5 22 1.0 23 1.0 24 1.0 25 1.5 26 1.5 27 1.0 28 1.0 29 1.0 30 3.5 31 6.0 32 5.0 33 4.0 34 5.0 35 6.0 36 6.5 37 7.0 38 6.0 39 5.0 40 3.0 41 1.0 42 5.0 43 9.0 44 8.5 45 8.0 46 6.0 47 4.0 48 9.0 49 14.0 50 12.5 51 11.0 52 8.0 53 5.0 54 5.0 55 5.0 56 5.0 57 5.0 58 3.0 59 1.0 60 2.0 61 3.0 62 1.5 63 0.0 64 0.5 65 1.0 66 2.0 67 3.0 68 2.0 69 1.0 70 1.5 71 2.0 72 1.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 114.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.12280701754386 #Duplication Level Percentage of deduplicated Percentage of total 1 99.11504424778761 98.24561403508771 2 0.8849557522123894 1.7543859649122806 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 2 1.7543859649122806 No Hit CACCACCAGTACTCCTGAAGAGTCTCACCTTTCCAGGCGAAAACAGCGGCG 1 0.8771929824561403 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTGATACCCCCCCTACTCC 1 0.8771929824561403 No Hit TATAAAGGAAGGGTGATGATCATTTCTTGGGTTGGAACTTGGATTTGATGT 1 0.8771929824561403 No Hit ATGCCGAGGCACGCCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCG 1 0.8771929824561403 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 1 0.8771929824561403 No Hit ATCAAGATGGTAGCAAGAAAGAAGCTATTCCTGTCTCTTATACACATCTCC 1 0.8771929824561403 No Hit CTTATGTAGCCACCAAAGGCAATTGCAAAAATTAACCCTTGATCATCATAT 1 0.8771929824561403 No Hit CTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCT 1 0.8771929824561403 No Hit CTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGG 1 0.8771929824561403 No Hit CATGAGTAGCGTGTGACGGGTGGGAATCCCGTCCGCCGATTATCCAAGGGT 1 0.8771929824561403 No Hit GTGTAAGCAAGAGTGAGTTACTTGACCGTGAAGCTGAAGATCCTGCTGTAC 1 0.8771929824561403 No Hit GTATTAGTACTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCCGTCTT 1 0.8771929824561403 No Hit GCTTAAATATGTTGAAGAGGAGAATTTGGGACAGATGGTCCATTGATTCAT 1 0.8771929824561403 No Hit CGTCTTTGCCGCGTCAAGGCTCGGGAAATACGTCGGCTCCATAAATATCTG 1 0.8771929824561403 No Hit GTCTCTACTTACTTGAAGTTGACATCAATCACTCACGTCTGTTGTTGTATT 1 0.8771929824561403 No Hit GCTTAGCGAGAATAATCGCTCCTCTAATGGCCGTAGCAACTTCCTTAGAGC 1 0.8771929824561403 No Hit CGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTATACCATCTC 1 0.8771929824561403 No Hit CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGATTTTAAGAAAAAGAAAGA 1 0.8771929824561403 No Hit GTGGGATTCTCGCTGTGTTAGGCTGCGTCGATAACTCTCAAGCTAAACGTT 1 0.8771929824561403 No Hit TTTCTTAACCCAAGCAATCAGTTTCGGAACCTCAGATTCGATACTGAAGTT 1 0.8771929824561403 No Hit AACTTCGAATTGGGAGGGCGATCCTCCCGGTGAACTGACCGTACCCCAAAC 1 0.8771929824561403 No Hit AAACAACGGCGGAGATAGAATTCGGTCTGCGGAGGAGGAAGCGGAAGCGAA 1 0.8771929824561403 No Hit GGATTAACGAGATTCCCACTGTCCCTGTCTACTATCCAGCGAAACCACAGC 1 0.8771929824561403 No Hit CTGTAACCTCTGCCGATGATTCCTCTGTTGCTACCTTTGGCACCTCCTGTT 1 0.8771929824561403 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTGGTTTTTACCCCCCCCTTTA 1 0.8771929824561403 No Hit CCGCCAGCCTGATGGACAGCGAGCGCCAGGCGTTTTCGCCCATGCCTGTCT 1 0.8771929824561403 No Hit GTGGTGGCAACGTTGGTGACTCTGTCACTATTCCTCTGCCTCAGTGGGTTG 1 0.8771929824561403 No Hit CGACCAGTGAGCTATTACGCACTCTTTGAAGGGTGGCTGCTTCTAAGCCAA 1 0.8771929824561403 No Hit GCGTAACCCACGTCCACCTTGCATTCCAAAGTCAGTTGGTTTTCAGAGGTT 1 0.8771929824561403 No Hit GTTATTTGTAACGTTGAACATCATGTTGGTGATCGTGGTGTTTTCGCTAGA 1 0.8771929824561403 No Hit TAATAAAATAGGACGTGCGGTTCTATTTTGTTGGTTTTTGGAATCGCCGTA 1 0.8771929824561403 No Hit GTGTGTGTGGCTGCCGTCGCATGCTGTTGATGTTGTCGTTAGCGGAAGTCG 1 0.8771929824561403 No Hit TCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGAAG 1 0.8771929824561403 No Hit AACAATGTACCTTCTTGTCCAGTGATATCAAATTGATATGTGAAGTTGCTG 1 0.8771929824561403 No Hit GACTAAACCTGTAAAACATGCTTCTACAGTCTGCATGCCTCAGCCCTCAGG 1 0.8771929824561403 No Hit GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC 1 0.8771929824561403 No Hit GAGTGGAGTGCTGGTGTTATCCTCTACATTTTACTCAGCGGTGTTCCTCCC 1 0.8771929824561403 No Hit ATTCTACACTTAGCATCCTTGCCCCACGGTCCAAGGGCAGGAGTCACCCTG 1 0.8771929824561403 No Hit CCTTGGACGCGCCGGGCAGTTCCTTATCGACCATCGCCACCACTTCCGGCC 1 0.8771929824561403 No Hit GATCTTCAGATGGAGAGGAAAGCAATGGAGATGAAGTAGAGACTGATGATG 1 0.8771929824561403 No Hit CTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAG 1 0.8771929824561403 No Hit GGAATGCACGCATTAATAATCTATGATGATCTTAGTAAACAGGCGGTGGCA 1 0.8771929824561403 No Hit GGGTAGATCACCAGGTTTCGCGTCTACTACTACCAACTATTGTCGCCCTAT 1 0.8771929824561403 No Hit TCTTTAAACCTCCAAAGAACAAATCTTCAAGTGGTGGTTTCTCACTTTCTA 1 0.8771929824561403 No Hit GGCTTAAGCCGCTCTTCCCATTTCCTCGCTGACCCATCATGCAAAAGGTAC 1 0.8771929824561403 No Hit ACCCGGTGTTGCTGAAGGTGTGAAAGACGAGATTTTTCTTTTGCTCTTCAT 1 0.8771929824561403 No Hit GGGTCATTCTATGAAGAAGAAGAGGAAGAGGATGATGGTGCAAAAGGTAGT 1 0.8771929824561403 No Hit CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATG 1 0.8771929824561403 No Hit TCCCATTTCCTCGCTGACCCATCATGCAAAAGGTACGCCGTTAGAGCTGTC 1 0.8771929824561403 No Hit CTCTCTTGGAGCGAATTGGATGATCGGGCCGAGGGCAGCGTAGCGCCTCTT 1 0.8771929824561403 No Hit GGGAGCTTTCCCCGGGCGTCGAACAGTCAGCTCAGAACTGGTACGGACAAG 1 0.8771929824561403 No Hit GCTACAAATCTCTCATTTCACCATCTAAACATTTTACATTCACTTTTTCCA 1 0.8771929824561403 No Hit CCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGG 1 0.8771929824561403 No Hit TTCCGCTACTGACACTTGAAAAAGCAGCTTTCAAGGTCTCGTCGCTTCGGT 1 0.8771929824561403 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGTTCCCCCCCCG 1 0.8771929824561403 No Hit GAGTTACTTCGTACCTTGACTGAAGATTCCTAAAGGGCGCTGTGAACTGTA 1 0.8771929824561403 No Hit GTGCATTGCACGGTGGATGTGAAGAAGTAGGCCATTATCTCGGCAATAATG 1 0.8771929824561403 No Hit TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTT 1 0.8771929824561403 No Hit GGGCCAGGTTTCCCCATTCGGACATCCTCGGATCAACGCTTTATTGACAAC 1 0.8771929824561403 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTCCA 1 0.8771929824561403 No Hit CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCGGGGTTTAACCCCT 1 0.8771929824561403 No Hit ATATATGGGAATGCCAATGAGAAGTTGGAAGCTGTAAATAGATTATTCAAT 1 0.8771929824561403 No Hit TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC 1 0.8771929824561403 No Hit CCTTCGGAATTTGAAGCTAGAGGTGTCAGAAAAGTTACCACAGGGATAACT 1 0.8771929824561403 No Hit CTCCTCATCTTCCTCGGTCTTTTCTTGAAGCTCCTCTAGCAGAGTAATCTT 1 0.8771929824561403 No Hit GCTCGTAAGCCCTGGATCGCTGTTGGAATGAGGAACGTTGACTACAAGAGA 1 0.8771929824561403 No Hit ACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAG 1 0.8771929824561403 No Hit TAATAAATCCCATGTTTGTGTTACTTCCGGTTGCACCCTTTTAGTTTGGTA 1 0.8771929824561403 No Hit GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAG 1 0.8771929824561403 No Hit GGCTCTGACCGCGTGCCTGTTGAAGAATGAGCCGGCGACTCATAGGCAGTG 1 0.8771929824561403 No Hit CCTGGAGATAGCTAGTTCTCCCCGAAATGTATTGAGGCTGTCTCTTATACA 1 0.8771929824561403 No Hit GTTGTGATGTTCATAGCAAGTTTATCCATATTCTTGCGTATAAGCTCTTGA 1 0.8771929824561403 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTGAAAACCAC 1 0.8771929824561403 No Hit GGTGAAAAGAACCCTATGACGGGAGTGCAATAGAGAACCTGAGATCCGATG 1 0.8771929824561403 No Hit GCTAAATACTCCTGGGTGACCGATAGCGAAGTAGTACCGTGAGGGAAGGGT 1 0.8771929824561403 No Hit GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA 1 0.8771929824561403 No Hit AGGTTCGGGAATGAAGATCTCGATCAGGCAGTTTGGACCTGGAATGATGCA 1 0.8771929824561403 No Hit CCTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAG 1 0.8771929824561403 No Hit GCCCAATGGTGGCTTGATGATGCCGTATGATCTAAAGGAGCTTGTCAAAGG 1 0.8771929824561403 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGT 1 0.8771929824561403 No Hit CGTCCCTCTCTCTTCGATGCAGAACCCGCAAAAAACTCATCCACACTCAAA 1 0.8771929824561403 No Hit GGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCTGTC 1 0.8771929824561403 No Hit GGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCAC 1 0.8771929824561403 No Hit GTATGCTCTGAGGCTAAAGATCTTGTTGAGATGGGGAAGGTCCAACTGGCT 1 0.8771929824561403 No Hit ATCTTCCTCTTCCAATCCATATCAACCATTTTTATTTTTTTAAGGTCTGTG 1 0.8771929824561403 No Hit GTGTGAATATGAATCACGTAAAGGTAGAAGAGAAGATGTGTGCAGCTGTTT 1 0.8771929824561403 No Hit CAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGTACTCTG 1 0.8771929824561403 No Hit GTGGTGATCGTCTCACTCGTGCCTCCAAGGTGTTGGAACAGCTCAGTGGTC 1 0.8771929824561403 No Hit ATCGTACGCTAAGGTCCCTAAGCAATCACTTAGTGGAAAAGGAAGTGATCG 1 0.8771929824561403 No Hit ATTCGACGAAGTTTTTTAAGAGAGAAGAAGGAATCTTCAGATGGGAATAGC 1 0.8771929824561403 No Hit ATTCTATTGAGTCTGACTCATAGTGATCATTTATCAAAGAATGACTCTGGT 1 0.8771929824561403 No Hit TATTAACAGCTACTCTAGGAGGAATGTTTTTATGTGGTGCTAACGATTTAA 1 0.8771929824561403 No Hit AAAGAGGAGCCCACCATGAAACCGACGAAACAGGTTTCTTACGGGCTGGAT 1 0.8771929824561403 No Hit GCTCGATAATGATCTTCCAAAACCATGTTAGCACCAGTCATGGTTCCAATG 1 0.8771929824561403 No Hit CCTTTCTATCACTTGAAGATTATCTACGCAATTCGTAAATGATCAAACTAG 1 0.8771929824561403 No Hit ATGGAACACTTTCTCCAATAGTCGATGACCCTGATCGCGCCATCATGTCTG 1 0.8771929824561403 No Hit CATTACATTCATTTCCGAACGTAGGAAGAAACTTGGGTTGGAGTAAATCTC 1 0.8771929824561403 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTATACC 1 0.8771929824561403 No Hit ACTTTAGTCCGGCGGTTTCTCAATCTGGTGATTTCTTAGCCGTTGCTTCTT 1 0.8771929824561403 No Hit CAAGGATACTTTAAACGTCCTGAAATCACAGAGGAGGCAATCACGAAGGAT 1 0.8771929824561403 No Hit AGAGTGGGCGATGGCGAGTTTGTTGAACCACCCAAAAGTTTTGGACAAAGT 1 0.8771929824561403 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 1 0.8771929824561403 TruSeq Adapter, Index 12 (95% over 21bp) CAAAGAGGGTATTCCTCCGGACCAGCAGAGATTGATCTTCGCCGAAAAACA 1 0.8771929824561403 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 1 0.8771929824561403 No Hit GGCATAGCGAGATCAAAGTGCTCACGAGGAGGATTACACCAACCACCATTG 1 0.8771929824561403 No Hit CGTTTGATCGTTGCTATCAGTTCTAACAATATCAAATAAAAATTTGAAAGT 1 0.8771929824561403 No Hit ACTTAATCTTGATTCGATTGAACACTTAGCGTGAATGATAAAGAGAAACTA 1 0.8771929824561403 No Hit TCTCCAGAGATCTGAATCTCTTTCCGATAATCCATCTCTCACTCTTCTACC 1 0.8771929824561403 No Hit ATCATTGACTTGGTGAGCCGTTACCTCACCTGTCTCTTATACACATCTCCC 1 0.8771929824561403 No Hit TGCCAAGGGTGCTGCTAACTTCACCTCCCAGGTCATCATCATGAACCACCC 1 0.8771929824561403 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1 0.8771929824561403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 1 0.8771929824561403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.8771929824561403 31 0.0 0.0 1.7543859649122806 32 0.0 0.0 1.7543859649122806 33 0.0 0.0 1.7543859649122806 34 0.0 0.0 1.7543859649122806 35 0.0 0.0 1.7543859649122806 36 0.0 0.0 1.7543859649122806 37 0.0 0.0 1.7543859649122806 38 0.0 0.0 2.6315789473684212 39 0.0 0.0 3.508771929824561 >>END_MODULE >>Kmer Content pass >>END_MODULE