##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_1.34100000009f12.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28393 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.958475680625504 38.0 38.0 38.0 32.0 38.0 2 36.96340647342655 38.0 38.0 38.0 32.0 38.0 3 36.89117035889127 38.0 38.0 38.0 32.0 38.0 4 36.964040432500965 38.0 38.0 38.0 32.0 38.0 5 36.96713978797591 38.0 38.0 38.0 32.0 38.0 6 38.90279294192231 40.0 38.0 40.0 38.0 40.0 7 38.85700700876977 40.0 38.0 40.0 38.0 40.0 8 38.82414679674568 40.0 38.0 40.0 38.0 40.0 9 38.79914063325467 40.0 38.0 40.0 38.0 40.0 10 38.79357588137921 40.0 38.0 40.0 38.0 40.0 11 38.83171908569013 40.0 38.0 40.0 38.0 40.0 12 38.80540978410171 40.0 38.0 40.0 38.0 40.0 13 38.78554573310323 40.0 38.0 40.0 38.0 40.0 14 38.78399605536576 40.0 38.0 40.0 38.0 40.0 15 38.762265347092594 40.0 38.0 40.0 38.0 40.0 16 38.787236290635015 40.0 38.0 40.0 38.0 40.0 17 38.78779980981228 40.0 38.0 40.0 38.0 40.0 18 38.83876307540591 40.0 38.0 40.0 38.0 40.0 19 38.81995562286479 40.0 38.0 40.0 38.0 40.0 20 38.80882611911387 40.0 38.0 40.0 38.0 40.0 21 38.81090409608002 40.0 38.0 40.0 38.0 40.0 22 38.80220476878104 40.0 38.0 40.0 38.0 40.0 23 38.808614799422394 40.0 38.0 40.0 38.0 40.0 24 38.82654175324904 40.0 38.0 40.0 38.0 40.0 25 38.774451449300884 40.0 38.0 40.0 38.0 40.0 26 38.74271827563132 40.0 38.0 40.0 38.0 40.0 27 38.72461522206178 40.0 38.0 40.0 38.0 40.0 28 38.656077202127285 40.0 38.0 40.0 38.0 40.0 29 38.54154192934878 40.0 38.0 40.0 38.0 40.0 30 38.499630190539925 40.0 38.0 40.0 38.0 40.0 31 38.35008628887402 40.0 38.0 40.0 38.0 40.0 32 38.21286936921072 40.0 38.0 40.0 38.0 40.0 33 37.95107949142394 40.0 38.0 40.0 38.0 40.0 34 37.82404113689994 40.0 38.0 40.0 32.0 40.0 35 37.69179022998627 40.0 38.0 40.0 32.0 40.0 36 37.4433134927623 40.0 38.0 40.0 32.0 40.0 37 37.375127672313596 40.0 38.0 40.0 32.0 40.0 38 37.214031627513826 40.0 38.0 40.0 32.0 40.0 39 36.9938365089987 40.0 38.0 40.0 27.0 40.0 40 36.84263726974959 40.0 38.0 40.0 27.0 40.0 41 36.66287465220301 40.0 38.0 40.0 27.0 40.0 42 36.425386538935655 40.0 38.0 40.0 27.0 40.0 43 36.199344908956434 40.0 38.0 40.0 27.0 40.0 44 35.94040784700454 40.0 38.0 40.0 13.0 40.0 45 35.60824851195717 40.0 38.0 40.0 13.0 40.0 46 35.24453210298313 40.0 38.0 40.0 13.0 40.0 47 34.85633782974677 40.0 38.0 40.0 13.0 40.0 48 34.51428168914873 40.0 38.0 40.0 13.0 40.0 49 34.237417673370196 40.0 32.0 40.0 13.0 40.0 50 33.92279787271511 40.0 32.0 40.0 13.0 40.0 51 33.34941710985102 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.4366689346150565 2201 2 -0.6588201743670226 2201 3 0.16270652105595218 2201 4 -0.5782324260298495 2201 5 0.634996497608519 2201 6 -0.7306582308739351 2201 7 -0.2194309549012985 2201 8 0.07746299741186391 2201 9 -0.4329853349364896 2201 10 0.3083124168214795 2201 11 -0.8818979107738727 2201 12 -0.28945244221396393 2201 13 -0.0637096049887731 2201 14 0.19200864759938696 2201 15 0.05300349260762971 2201 16 0.4845235859080006 2201 17 0.2360791030427407 2201 18 0.5824436192242146 2201 19 0.7062001228079424 2201 20 0.7359903923276576 2201 21 -0.06716639938038327 2201 22 0.137933815070177 2201 23 -0.5253898468295688 2201 24 0.36488715778585856 2201 25 0.5155421983410093 2201 26 0.43077418797936673 2201 27 -0.3321527361270711 2201 28 -0.1617109002832322 2201 29 -0.20298377769307052 2201 30 0.38903394843387673 2201 31 -0.7813242501708473 2201 32 0.4525670356296132 2201 33 0.959518608174271 2201 34 1.1568132612175788 2201 35 0.6871703636580406 2201 36 0.5630998373223477 2201 37 0.10125007942205144 2201 38 -0.1020879663773826 2201 39 0.2887383409827322 2201 40 -0.8293241616638767 2201 41 0.24821409824453866 2201 42 -0.09610721663779742 2201 43 -0.5331193995206434 2201 44 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30 0.8279228373227596 1101 31 1.0575646387180413 1101 32 1.027567035629616 1101 33 -0.06825916960350753 1101 34 0.32347992788424307 1101 35 -0.06005185856417938 1101 36 -0.648011273788768 1101 37 -0.35708325391128426 1101 38 0.2673564780670574 1101 39 -0.07792832568393493 1101 40 0.45678694944723475 1101 41 0.09821409824454008 1101 42 0.14278167225108973 1101 43 -0.8386749550762005 1101 44 -0.3322228986281104 1101 45 0.12121398818906215 1101 46 0.9775079851425374 1101 47 1.5176808069280128 1101 48 1.1519352895334052 1101 49 1.376751658132548 1101 50 1.6801956385462233 1101 51 2.257959795730045 2202 1 0.27731220946886026 2202 2 -0.20267982348983082 2202 3 0.2138761116992285 2202 4 -0.5197528938661051 2202 5 0.15254035725764226 2202 6 0.3380552194184645 2202 7 -1.5162145806322869 2202 8 -0.08481770434252667 2202 9 0.2804649574611702 2202 10 -0.3086466475060021 2202 11 0.4806751886413281 2202 12 0.4678574993065041 2202 13 0.4991558920872521 2202 14 0.1335291154356426 2202 15 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2202 51 2.7711176904668875 1102 1 -0.8818065175813246 1102 2 0.4264511434624296 1102 3 -0.2836759078846143 1102 4 0.2189251967091721 1102 5 -0.008414355104655158 1102 6 0.3307112781699928 1102 7 -1.3001803089064623 1102 8 0.017385478032018398 1102 9 0.21559347643302118 1102 10 0.43492740390158247 1102 11 0.2628049316033838 1102 12 0.16920388853538526 1102 13 0.24701390922311361 1102 14 -0.10514897513963462 1102 15 0.07109134790479033 1102 16 -0.2622464399317934 1102 17 0.26450287565255337 1102 18 -1.117814778708599 1102 19 0.32764715123170873 1102 20 -0.6193067652950859 1102 21 0.1272780450640596 1102 22 -0.3181385363510074 1102 23 -0.30510560910347095 1102 24 0.45403444460756504 1102 25 0.3540434903306746 1102 26 -0.6519131505219278 1102 27 -0.37672637953792076 1102 28 -0.8755351896889181 1102 29 -0.7591853280806617 1102 30 -0.7744027440725887 1102 31 -0.7877841984912664 1102 32 -0.5067352899517843 1102 33 -0.6729103323942027 1102 34 0.026968299977269794 1102 35 -0.06412162600604177 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-1.9482130505527593 1213 41 -0.35178590175546276 1213 42 -1.027218327748912 1213 43 -0.3386749550762005 1213 44 0.27777710137188905 1213 45 -1.5037860118109379 1213 46 -0.4724920148574654 1213 47 -0.7423191930719852 1213 48 -1.8230647104665962 1213 49 -1.0632483418674568 1213 50 -1.53480436145378 1213 51 -0.8120402042699553 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 3.0 17 3.0 18 2.0 19 2.0 20 3.0 21 7.0 22 12.0 23 21.0 24 42.0 25 89.0 26 136.0 27 165.0 28 262.0 29 369.0 30 516.0 31 664.0 32 775.0 33 911.0 34 1109.0 35 1257.0 36 1453.0 37 2136.0 38 3152.0 39 15303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.899537902571524 16.173409996825285 12.257927969240539 19.669124131362658 2 16.845701405275946 22.614728982495684 39.74923396611841 20.790335646109956 3 22.114605712675658 30.278589793258902 25.95710210263093 21.64970239143451 4 13.425844398267179 16.72595358010777 34.367625823266295 35.48057619835875 5 17.89173387806854 35.00862888740183 28.147782904236962 18.95185433029268 6 30.091924065790863 35.33617440918536 14.528228788785968 20.043672736237806 7 29.024759623850947 31.109780579720354 20.860775543267707 19.004684253160992 8 29.5460148628183 26.545275243898143 18.659528757088015 25.249181136195542 9 29.1233754798718 15.697531081604621 21.579262494276758 33.599830944246825 10 22.95988447856866 24.498996231465505 24.99207551156975 27.549043778396083 11 32.06424118620787 24.85471771211214 23.60793153241996 19.47310956926003 12 29.602366780544497 24.284154545134363 27.309548128059735 18.8039305462614 13 30.01444017891734 23.00919240657909 27.436339942943683 19.540027471559892 14 24.26302257598704 21.205931039340683 31.24713837917797 23.28390800549431 15 22.600641003064133 25.28087908991653 29.415701053076464 22.702778853942874 16 20.779769661536292 29.05997957242982 27.971683161342586 22.188567604691297 17 20.600147923784032 24.74201387665974 27.88011129503751 26.777726904518715 18 24.481386257176062 21.737752262881695 31.733173669566444 22.047687810375795 19 26.819990842813368 22.248441517275385 31.800091571866307 19.131476068044943 20 24.097488817666328 21.512344591976895 31.881097453597718 22.50906913675906 21 24.016482935934913 22.551333075053712 33.31454936075794 20.11763462825344 22 21.396118761666607 24.65748600007044 33.159581587010884 20.78681365125207 23 21.674356355439723 25.728172436868242 33.98725037861445 18.61022082907759 24 21.783538196034232 26.330433557567005 33.22649948931075 18.659528757088015 25 19.589335399570317 27.291938153770296 34.52259359701335 18.59613284964604 26 18.77927658225619 28.278096713978794 34.40988976156095 18.532736942204064 27 18.45877505018843 28.943753742119537 33.522347057373295 19.07512415031874 28 18.131229528404887 28.383756559715422 32.53266650230691 20.952347409572784 29 18.360159194167576 29.679850667418023 31.87405346388194 20.085936674532455 30 18.77927658225619 28.96136371640897 32.08537315535519 20.173986545979645 31 19.46958757440214 28.204134821963162 31.289402317472614 21.036875286162083 32 19.08216814003452 27.939985207621596 31.881097453597718 21.09674919874617 33 18.955376325150567 28.718346071214736 32.51505652801747 19.81122107561723 34 19.367449723523404 29.67632867256014 31.268270348325288 19.687951255591166 35 18.83915049484028 30.401859613284966 31.419716127214453 19.3392737646603 36 19.208959954918466 30.172929947522277 31.733173669566444 18.884936427992816 37 18.599654844503927 31.56763991124573 30.982988764836406 18.84971647941394 38 18.0995315746839 31.0111647236995 31.683865741556016 19.20543796006058 39 17.624062268869086 31.29644630718839 30.98651075969429 20.09298066424823 40 18.081921600394463 31.416194132356566 31.095692600288803 19.406191666960165 41 18.194625435846863 30.85971894481034 30.2504138343958 20.695241784946994 42 18.152361497552214 30.687141196773855 30.060226112069877 21.100271193604055 43 17.58884232029021 30.532173423026805 29.757334554291553 22.121649702391437 44 16.849223400133837 30.532173423026805 30.313809741837776 22.304793435001585 45 16.990103194449336 30.148275983517063 29.299475222766176 23.562145599267424 46 17.764942063184588 29.391047089071247 28.96840770612475 23.875603141619415 47 16.754129538970876 29.40161307364491 28.989539675272074 24.85471771211214 48 16.613249744655374 28.901489803824887 28.475328426020496 26.009932025499243 49 17.285950762511888 28.760610009509385 28.059733032789776 25.893706195188955 50 17.13098298876484 27.96463917162681 28.690170112351637 26.214207727256717 51 17.444440531116825 27.80967139787976 27.390554009791146 27.35533406121227 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 47.0 1 32.0 2 17.0 3 18.0 4 19.0 5 27.0 6 35.0 7 24.0 8 13.0 9 31.0 10 49.0 11 105.5 12 162.0 13 357.5 14 553.0 15 743.5 16 934.0 17 930.5 18 927.0 19 903.5 20 880.0 21 879.5 22 879.0 23 1004.0 24 1129.0 25 1099.0 26 848.5 27 628.0 28 532.5 29 437.0 30 448.5 31 460.0 32 585.5 33 711.0 34 721.5 35 732.0 36 644.5 37 557.0 38 512.5 39 468.0 40 507.0 41 546.0 42 659.0 43 772.0 44 915.5 45 1059.0 46 1173.5 47 1288.0 48 1480.0 49 1672.0 50 1730.0 51 1788.0 52 1754.5 53 1721.0 54 1762.0 55 1803.0 56 1667.5 57 1532.0 58 1446.5 59 1361.0 60 1189.0 61 1017.0 62 934.5 63 852.0 64 711.5 65 571.0 66 516.5 67 462.0 68 421.0 69 380.0 70 325.0 71 270.0 72 247.5 73 225.0 74 189.0 75 129.5 76 106.0 77 84.0 78 62.0 79 42.5 80 23.0 81 20.5 82 18.0 83 11.0 84 4.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1549677737470503 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 28393.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.13111103952208 #Duplication Level Percentage of deduplicated Percentage of total 1 91.88561958401779 70.8723992707016 2 5.00305891268545 7.717829850632242 3 1.2842692970668275 2.971713532701313 4 0.5814729336794734 1.793986136564325 5 0.3555078097018713 1.3710356172766163 6 0.1993868154076408 0.9227355959414062 7 0.14507902031127567 0.7833074217593858 8 0.08536536179770417 0.5267460159798157 9 0.07960682813907519 0.5526146790629244 >10 0.31207270790924757 4.907928392667002 >50 0.022853576428405336 1.314990418885913 >100 0.04570715285522023 6.264713067827464 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 289 1.0178565139294897 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 281 0.9896805550663896 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 221 0.7783608635931392 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 190 0.6691790229986264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 173 0.6093051104145388 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 144 0.507167259535801 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 140 0.49307928010425106 RNA PCR Primer, Index 27 (95% over 21bp) GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 131 0.4613813263832635 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 105 0.36980946007818827 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 103 0.36276547036241324 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97 0.34163350121508823 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 93 0.32754552178353824 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 71 0.25006163491001304 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 57 0.2007537068995879 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55 0.1937097171838129 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.17257774803648787 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45 0.15848976860493783 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.1549677737470503 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 44 0.1549677737470503 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 42 0.14792378403127532 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 42 0.14792378403127532 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 39 0.1373577994576128 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.1338358045997253 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT 36 0.12679181488395025 No Hit GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 34 0.11974782516817525 No Hit GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.11974782516817525 No Hit GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.11622583031028774 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33 0.11622583031028774 No Hit ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 30 0.10565984573662522 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 30 0.10565984573662522 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0035219948578875076 2 0.0 0.0 0.0035219948578875076 3 0.0 0.0 0.0035219948578875076 4 0.0 0.0 0.0035219948578875076 5 0.0 0.0 0.0035219948578875076 6 0.0 0.0 0.0035219948578875076 7 0.0 0.0 0.0035219948578875076 8 0.0 0.0 0.0035219948578875076 9 0.0 0.0 0.0035219948578875076 10 0.0 0.0 0.0035219948578875076 11 0.0 0.0 0.007043989715775015 12 0.0 0.0 0.007043989715775015 13 0.0 0.0 0.007043989715775015 14 0.0 0.0 0.007043989715775015 15 0.0 0.0 0.007043989715775015 16 0.0 0.0 0.007043989715775015 17 0.0 0.0 0.010565984573662522 18 0.0 0.0 0.010565984573662522 19 0.0 0.0 0.010565984573662522 20 0.0 0.0 0.01408797943155003 21 0.0 0.0 0.024653964005212553 22 0.0 0.0 0.038741943436762585 23 0.0 0.0 0.05987391258408763 24 0.0 0.0 0.09157186630507519 25 0.0 0.0 0.10918184059451273 26 0.0 0.0 0.1408797943155003 27 0.0 0.0 0.1937097171838129 28 0.0 0.0 0.36980946007818827 29 0.0 0.0 0.7149649561511641 30 0.0 0.0 1.165780297960765 31 0.0 0.0 1.665903567780791 32 0.0 0.0 2.1942027964639172 33 0.0 0.0 2.6837600817102807 34 0.0 0.0 3.275455217835382 35 0.0 0.0 3.775578487655408 36 0.0 0.0 4.395449582643609 37 0.0 0.0 5.156200471947311 38 0.0 0.0 6.043743176134963 39 0.0 0.0 7.265875391821928 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTGG 55 3.979909E-6 28.636364 4 ACGGGTG 60 7.829694E-6 26.249998 3 TACGGGT 80 2.808827E-6 22.5 2 CGGGCAG 55 0.0038440565 20.454546 4 ACGGGCA 55 0.0038440565 20.454546 3 GGGCAGT 60 0.006358739 18.749998 5 TACGGGC 60 0.006358739 18.749998 2 AACGCAG 440 0.0 15.852273 6 GTACGGG 430 0.0 14.651163 1 CGCAGAG 495 0.0 14.090909 8 ACGCAGA 510 0.0 13.676471 7 CAGAGTA 525 0.0 13.285715 10 AGAGTAC 525 0.0 13.285715 11 GCAGAGT 535 0.0 13.037384 9 CCCCCCC 145 4.5954614E-4 12.413794 44 AGTACGG 290 1.1299562E-8 11.637932 13 GAGTACG 295 1.4555553E-8 11.440679 12 GTATCAA 670 0.0 11.417911 1 CGGGGTG 160 0.0010509652 11.25 4 GGGGTGG 160 0.0010509652 11.25 5 >>END_MODULE