FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_96.34100000009f05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_96.34100000009f05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335919
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC4340.1291978125679107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA458.6778164E-734.9988419
GCGCGAG554.154481E-628.63541837
CGCCACG406.15818E-428.12406720
TAGGAGT856.4483174E-926.4697134
GCCCACG608.17111E-626.2491318
CTCGTAT608.17111E-626.2491339
GAGACCC608.17111E-626.2491324
CGGCAGG551.3656954E-424.54464335
CGTATGC751.6921749E-623.99920541
TATGCCG751.6921749E-623.99920543
TCGCGCG702.696332E-522.4992569
TCGGCAG602.4630205E-422.49925434
ACTGTCC500.002261734622.4992548
GTCCCGG500.002261734622.49925426
CGTCCCG500.002261734622.49925425
TGTAGGA1156.728442E-921.5210292
GTGTAGG1301.196895E-920.7995051
GTCTTAT654.18824E-420.7995051
TTATCCT654.2305756E-420.7685454
GGCGCGA654.2305756E-420.76854536
Produced by FastQC (version 0.11.2)