FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_95.34100000009ef7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_95.34100000009ef7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372718
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC12740.34181338169876424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.13200328398413813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2250.030.99935343
ATGCCGT2250.030.99935344
CGTATGC2200.030.6811841
AGGTGTC300.005143374729.999377
TATCTCG2350.029.68023136
TGCCGTC2400.029.06189245
GTATGCC2400.028.1244142
ACGAGAC2400.028.1244122
CCCACGA2450.027.55044419
GCCCACG2550.027.3523718
CACGAGA2500.026.99943721
TCGTATG2500.026.99943740
CGAGACA2450.026.63209723
CTCGTAT2600.025.96099739
TCTCGTA2650.025.47116738
GTATTAA801.0404983E-725.3357641
CCACGAG2750.024.54494320
CCGAGCC2900.024.05122414
ATCTCGT2850.023.68371637
AGCCCAC2850.023.68371617
Produced by FastQC (version 0.11.2)