FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_94.34100000009eea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_94.34100000009eea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483491
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC5300.10961941380501394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.10610331939994748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1100.034.77480743
GTTAGAC453.487744E-529.998693
CTCGTAT1400.028.92730739
GTATGCC1550.027.57944142
CCCACGA1650.027.27153819
CGTATGC1500.026.99882141
AATCTCG1500.026.99882136
GCCCACG1600.026.71758518
CCGAGCC1700.026.4694314
TCGTATG1550.026.12789240
CTCCGAG1650.025.9079612
AGCCCAC1650.025.9079617
CGAGACT1800.024.99890923
ATGCCGT1650.024.54692344
TCCGAGC1900.023.68317813
TAATACG802.9699677E-622.499024
ATCTCGT1800.022.49901837
GACTTGC1800.022.49901826
CCACGAG1800.022.49901820
GAGCCCA2000.022.49901616
Produced by FastQC (version 0.11.2)