FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_93.34100000009edd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_93.34100000009edd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101314
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC13100.11894881931946748No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA12080.10968715552512726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGCC250.002107456335.9986577
CGCTATA2250.032.99876831
GACGCTA2250.032.99876829
GCCCACG2300.032.28140318
ACGACGC2450.032.1416627
CGACGCT2450.031.22332428
TATGCCG2400.030.93634443
CCGAGCC2400.030.93634414
CGAGACG2550.030.881223
TGCCGTC2450.030.3049945
CGTCGGG602.4196197E-729.99887814
CGTATGC2500.029.6988941
ATGCCGT2500.029.6988944
GCCAGGC654.8746733E-727.69127327
CGAGCCC2800.027.3204115
GTCGCGC507.1599505E-526.9989938
CGCGCGC507.1599505E-526.99899310
ACGCTAT2800.026.51686930
GGTCGCG608.193518E-626.249027
TACCCGG709.309988E-725.7133276
Produced by FastQC (version 0.11.2)