FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_92.34100000009ec0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_92.34100000009ec0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794260
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC16420.20673331151008487No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA10830.13635333517991588No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT10170.1280437136454058No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA9500.1196081887543122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2100.032.1415118
TATGCCG2100.032.1415143
GCGAATC2350.030.6370133
TCGCCCC300.00514736829.99874125
CGTATGC2250.029.99874141
CGTGACT300.00514736829.99874112
CGCGCTT453.4905002E-529.99874112
ATGCCGT2250.029.99874144
TGCCGTC2300.029.34659645
CCCACGA2350.028.72219819
GTATGCC2400.028.1238242
CGAGACC2400.028.1238223
CAAGGCG2450.027.54986229
GTCGCGC507.157705E-526.9988678
TCGCGCG507.157705E-526.9988679
GGCGAAT2600.026.82579832
TCGTATG2500.026.09890440
ACGAGAC2600.025.9604522
CCACGAG2600.025.9604520
CCGAGCC2650.025.4706314
Produced by FastQC (version 0.11.2)