FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_91.34100000009eb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_91.34100000009eb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996483
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC22070.22147894143703406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2500.039.5985445
ATGCCGT2750.035.99867644
TATGCCG2800.035.35584343
GCCCACG2850.034.73556518
CGAGACG2900.034.13667723
CCCACGA2950.033.55808619
CCGAGCC3050.032.4578214
CTCCGAG3100.031.93430912
CGAGCCC3150.031.42741615
CGTATGC3500.030.85600741
AGACGAT3300.030.6806925
TCTCGTA3250.030.46041538
CGATAAC3250.030.46041528
TCCGAGC3300.029.99889813
CCCGTAA453.4912595E-529.99889613
CCACGAG3350.029.55115120
ACGAGAC3450.029.34674822
TCGTATG3400.029.11657540
CACGAGA3550.028.52007921
TATCTCG3500.028.28467436
Produced by FastQC (version 0.11.2)