FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_9.341000000098e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_9.341000000098e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496234
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG5730.11546971791533832No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC5180.10438623713812435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA453.487421E-529.99932931
GCGGGGG406.1619724E-428.1243725
TATGCCG507.15143E-526.99939543
GGGCCTC450.001225732224.99944129
CCGACTG450.001225732224.99944129
GGGCTAA500.002249882822.5221921
AGTCCGG602.465142E-422.49949610
CGCGCGA602.465142E-422.49949610
CCTGTAC500.002263104522.4994963
TAAGGTG702.6994623E-522.4994965
GCCCACG654.2341946E-420.76876618
GTATGCC654.2341946E-420.76876642
GACGCTA654.2341946E-420.76876629
GGGAACG550.00393394420.4540885
GGCGGGG550.00393394420.45408824
ACGTCGG550.00393394420.45408819
CCTGGAC550.00393394420.4540883
CGGACGC550.00393394420.45408815
ACGCTAT706.9768674E-419.28528430
GTCGCGC851.2028978E-418.5289968
Produced by FastQC (version 0.11.2)