FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_89.34100000009e9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_89.34100000009e9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783163
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTAGACTCCCATGTAATAAGAGAATCTTGCATCCATCTTGGTTTTGAA10360.13228408390079716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCCC1600.028.1238027
GCCCACG1004.5474735E-1126.9988518
GCGACCT1101.5097612E-1024.54440930
GTATCAA9150.023.6508541
CGACCTA1003.6423444E-822.49904331
TGCGACC1003.6423444E-822.49904329
TATGCCG1204.5110937E-1022.4990443
TAGTACA802.9738458E-622.499044
CTTAGAC3700.021.2828773
TTAGACT3600.021.2490964
GGTATCA4250.021.2162061
CCCACGA1401.364242E-1020.89196819
CTATACT2050.020.8527724
ACTATAC1900.020.1307223
TGCCGTC1351.9626896E-919.9991545
CGTATGC1151.5944352E-719.56438641
TCCGAGC1503.43789E-1019.49917213
CGAGCCC1503.43789E-1019.49917215
ATGCCGT1302.7703209E-819.03765144
ACTGCGA1202.4952533E-718.749227
Produced by FastQC (version 0.11.2)