FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_87.34100000009e70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_87.34100000009e70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398971
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG352.8176975E-432.1416932
TATACTG300.005144189629.9989135
CGTCCCG406.160337E-428.12398125
CGCTTCA406.160337E-428.12398115
GTATATA654.792673E-727.7364941
GCCCACG709.2849587E-725.71335418
TATGCCG709.2849587E-725.71335443
CCCACGA709.2849587E-725.71335419
TACTATA709.2849587E-725.7133542
GCCACGT450.001225409724.99909421
CGTATGC751.6935519E-623.9991341
CTCGTAT751.6935519E-623.9991339
GTATGCC751.6935519E-623.9991342
GTATCAA8050.022.9558281
TCGTATG802.9676175E-622.49918640
GTCCCGG500.002262514322.49918626
AACTGTG602.464198E-422.4991847
CTAACGT550.00393292920.4538043
CCTATCT1009.0276626E-720.24926834
GGTATCA3150.020.0319141
Produced by FastQC (version 0.11.2)