FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_86.34100000009e63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_86.34100000009e63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294570
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC3260.11066978986319041TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTA300.005141780729.99920740
CTATCCC300.005141780729.9992079
GGTATCA2000.029.2840231
GTATGCC856.4410415E-926.46988942
GTATCAA6100.026.2189881
CGTATGC901.1837983E-824.9993441
TATGCCG901.1837983E-824.9993443
TCGTATG901.1837983E-824.9993440
ATGCCGT1001.3351382E-924.74934644
ACTACTT851.8842002E-723.82289927
GGTATTT500.002260990722.49940535
ACGTCCG602.4619093E-422.49940531
TAGATCG500.002260990722.4994055
GGTTCCG602.4619093E-422.49940531
TAACTGA500.002260990722.4994056
CCCACGA1056.070695E-821.42800719
TATCAAC7600.021.3152282
TAACCGG654.228681E-420.76868444
CGTCCCG654.228681E-420.76868425
GCCCACG1201.0972144E-820.62445418
Produced by FastQC (version 0.11.2)