FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_84.34100000009e3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_84.34100000009e3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913146
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC13410.1468549388597223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2400.033.74901218
TATGCCG2550.031.7637843
CCCACGA2600.031.15293519
ATGCCGT2650.030.56514444
TACGCGT406.166218E-428.1241825
CGTATGC3000.026.99921241
CCGAGCC3100.026.85405314
TCGTATG3050.026.55660240
CGAGACC2750.026.18105323
TCTCGTA3250.025.61463738
TGCCGTC3350.025.52164345
CTCGTAT3200.025.31176239
ACGAGAC3300.025.22653622
GTATGCC3300.024.54473942
GAGACCC2950.024.40606524
ACCCTGG3000.023.999327
CTCCGAG3500.023.78501912
CACGAGA3600.023.12432721
GTCCTAT602.445591E-422.5289521
CAGCCCC802.974397E-622.49934428
Produced by FastQC (version 0.11.2)