FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_83.34100000009e20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_83.34100000009e20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768408
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC453965.907798981790924No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT128721.675151742303568No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC104501.3599546074481266No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG41740.5432010077979407No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA41430.539166692694506No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA36310.4725354238893921No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG31870.41475362047245734No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC29410.38273937803875024No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA20260.26366201288898605No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA16930.220325660326285No Hit
AAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACGAG11190.14562576131430177No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA10490.13651601753235262No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG10130.13183100644449303No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC9780.12727613455351844No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC9580.12467335061581869No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC8430.10970734297404502No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC8180.10645386305192034No Hit
AAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC7940.10333052232668061No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA7720.10046745999521087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC47500.044.14583245
ATGCCGT60200.034.9074344
TATGCCG66000.031.83980643
CGTATGC66200.031.7436141
ATCTCGT66150.031.73359337
GTATGCC66300.031.69573242
TCGTATG66300.031.69573240
TATCTCG66200.031.67563636
TCTCGTA66400.031.61411338
CTCGTAT66650.031.46177539
CGAGACA66600.031.4516123
ACGAGAC67250.031.34835422
CACGAGA67350.031.26840221
AGACAAC67500.031.03225525
CAACATA67500.030.93225928
ACATAAT67650.030.89693330
GACAACA67900.030.84944726
ATATCTC67900.030.8163135
GAGACAA68350.030.6463424
ATAATAT68250.030.55937832
Produced by FastQC (version 0.11.2)