FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_82.34100000009e13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_82.34100000009e13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218798
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC356262.9230438514011343No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA71120.583525736012038No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA64190.526666436932125No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT60840.49918033997430256No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG48960.40170725583730854No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG48630.39899967016683646No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG44920.36855984338668096No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC44650.3663445460199311No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG42940.35231432936384866No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC42460.3483760229340711No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG40910.33565857508791447No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA37360.30653151711768484No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA33670.27625578643877No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG33490.27477892152760347No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG29400.241221268823874No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA28650.2350676650273466No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA28370.2327703196099764No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA28340.23252417545811527No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA25140.20626879925959837No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG23990.19683327343825635No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC22980.18854642032559948No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG22150.18173643212410917No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC18740.15375804686256458No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA17890.14678396255983353No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA17600.14440456909184293No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTAT17530.1438302327375004No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTA16980.13931758995338028No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA16490.1352972354729824No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA16180.13275374590375105No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA15810.12971796803079755No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA15600.12799495896776988No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACA15580.12783086286652917No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACA15560.1276667667652884No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTCC14700.120610634411937No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTAT14660.12028244220945554No Hit
GTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGAG14230.11675437603277984No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC14070.11544160722285399No Hit
ATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG14000.11486727086851144No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAACTGTCTCTT13810.11330835790672449No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC13720.11256992545114121No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC13670.11215968519803939No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC13320.1092880034263266No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC13290.1090418592744655No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC13160.10797523461640074No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTA13040.10699065800895637No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC12940.10617017750275272No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC12940.10617017750275272No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC12360.10141139056677152No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT12270.10067295811118825No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTAT12200.1000986217568457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC39050.044.30748745
ATGCCGT46950.036.85212744
GTATGCC51800.033.40168842
TATGCCG51850.033.36947643
CCACGAG52150.033.3500920
CGAGACT52050.033.3277123
ATCTCGT52050.033.24125737
CTCGTAT52200.033.14573739
TCTCGTA52250.033.11401738
GACTTGC52500.033.0420426
ACGAGAC52600.033.02222
TCGTATG52550.033.01060540
TTGCTGG52600.032.9364529
GCTGGAA52650.032.90517431
CCCACGA52650.032.90517419
AGACTTG52900.032.8347325
GAGACTT52850.032.82322324
CACGAGA53300.032.6727421
AATCTCG53200.032.48040436
TGCTGGA53550.032.3941630
Produced by FastQC (version 0.11.2)