FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_81.34100000009e06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_81.34100000009e06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259419
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA12000.4625721323418871No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA11560.44561115415601793No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG11380.4386725721708896No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC10960.42248254753892356No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG9720.37468342719692854No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC9040.34847100636422157No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC8850.3411469476021417No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC6970.26867731353524604No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT6650.25634205667279575No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG5680.21895080930849323No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG5620.21663794864678376No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT5500.21201222732336492No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT5270.20314626145347872No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA5260.20276078467652714No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT4720.18194503872114223No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA4710.18155956194419068No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC4600.17731931739772339No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA4580.17654836384382022No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA4370.1684533515278372No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA4330.16691144442003092No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT4140.15958738565795103No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT4130.15920190888099947No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA4090.15766000177319317No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA4070.15688904821929003No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT3990.15380523400367743No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA3800.14648117524159757No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCTC3780.14571022168769443No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG3730.14378283780293655No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT3600.13877163970256612No Hit
GCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA3520.13568782548695354No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG3460.1333749648252441No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCC3300.12720733639401896No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC3180.12258161507060006No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG3120.12026875440889064No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC2980.11487207953156861No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG2890.11140278853900448No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC2880.11101731176205289No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT2840.10947540465424661No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC2820.10870445110034346No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCCTGTCTCTTATACACA2800.10793349754644033No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTAT2740.10562063688473089No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC2700.10407872977692459No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC2670.10292229944606987No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG2640.10176586911521517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA403.4454752E-739.37348631
GACGCTA403.4454752E-739.37348629
CGTATGC702.0008883E-1135.3557841
CTCGTAT702.0008883E-1135.3557839
TATGCCG702.0008883E-1135.3557843
TCGTATG702.0008883E-1135.3557840
ATGCCGT702.0008883E-1135.3557844
TATATCT458.6680484E-734.9986534
GACGACG458.6680484E-734.9986526
TATCTCG653.6561687E-1034.6140536
GTATGCC801.8189894E-1233.748742
TGCCGTC754.7293724E-1132.99872645
ACGCTAT501.974402E-631.49878530
GTCGTAC651.3718818E-831.2067831
CGAGTCG602.4075962E-729.9988426
CGCGCTA300.005140968629.99884212
TTGTACT453.4814577E-529.9988424
CGGGCAA300.005140968629.99884217
TGCAGAG453.4814577E-529.9988428
ACGAGTC602.4075962E-729.9988425
Produced by FastQC (version 0.11.2)