FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_80.34100000009df8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_80.34100000009df8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878254
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC23210.26427434432407937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2850.039.4718445
GCCCACG4100.027.4377418
ATGCCGT4100.027.4377444
AGGGGCG507.158788E-526.9987357
TATGCCG4400.026.07832543
CAAGGCG3950.025.63171229
CCCACGA4400.025.56698419
GTATGCC4400.025.56698442
AGCGACC450.001226659224.998838
GGGCGAA551.3682783E-424.5443069
CCGAGCC4600.024.45537814
CCGTTAC1600.023.9555931
AGCCCAC4700.023.93505117
GCGAATC4150.023.85430533
AGGCGAA4300.023.5454131
CTCGTAT4750.023.2094439
CGTATGC4950.023.18073341
GGCGAAT4400.023.01028632
CGAGCCC4900.022.95810915
CCACGAG4900.022.95810920
Produced by FastQC (version 0.11.2)